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    LDHB lactate dehydrogenase B [ Homo sapiens (human) ]

    Gene ID: 3945, updated on 22-Sep-2016
    Official Symbol
    LDHBprovided by HGNC
    Official Full Name
    lactate dehydrogenase Bprovided by HGNC
    Primary source
    HGNC:HGNC:6541
    See related
    Ensembl:ENSG00000111716 HPRD:01026; MIM:150100; Vega:OTTHUMG00000133760
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
    Summary
    This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
    Orthologs
    Location:
    12p12.2-p12.1
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    108 current GRCh38.p7 (GCF_000001405.33) 12 NC_000012.12 (21635342..21657971, complement)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 12 NC_000012.11 (21788275..21810789, complement)

    Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene spexin hormone Neighboring gene glycogen synthase 2 Neighboring gene uncharacterized LOC105369689 Neighboring gene uncharacterized LOC102724261 Neighboring gene potassium voltage-gated channel subfamily J member 8

    GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify lactate dehydrogenase B (LDHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    env HIV-1 gp120/41 (SFVenvBX08)-expressing microglia exhibit a 170% increase in lactate dehydrogenase (LDH) release PubMed
    Envelope transmembrane glycoprotein gp41 env A lentivirus lytic peptide 1 (LLP-1) corresponding to the carboxyl terminus of HIV-1 gp41 induces a significant lactate dehydrogenase (LDH, a marker of cell death) release from human neuronal and glial cell lines PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify lactate dehydrogenase B (LDHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify lactate dehydrogenase B (LDHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify lactate dehydrogenase B (LDHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Tat tat Expression of HIV-1 Tat upregulates the abundance of lactate dehydrogenase B (LDHB) in the nucleoli of Jurkat T-cells PubMed
    tat Upregulation of actin, heat shock protein 90 and mitochondrial single-stranded DNA binding protein, and downregulation of lactate dehydrogenase are identified in human astrocytes expressing Tat PubMed
    capsid gag The interaction of HIV-1 CA with human cellular lactate dehydrogenase B protein (LDHB) is identified by yeast two-hybrid screen PubMed
    gag HIV-1 CA is strongly associated with LDHB in HIV-1-infected lymphocytic cells PubMed

    Go to the HIV-1, Human Interaction Database

    Products Interactant Other Gene Complex Source Pubs Description

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    L-lactate dehydrogenase activity EXP
    Inferred from Experiment
    more info
    PubMed 
    NAD binding IEA
    Inferred from Electronic Annotation
    more info
     
    identical protein binding IEA
    Inferred from Electronic Annotation
    more info
     
    kinase binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    NAD metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    lactate metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    oxidation-reduction process IEA
    Inferred from Electronic Annotation
    more info
     
    pyruvate metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IDA
    Inferred from Direct Assay
    more info
     
    cytoplasm TAS
    Traceable Author Statement
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    extracellular exosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    membrane raft IDA
    Inferred from Direct Assay
    more info
    PubMed 
    mitochondrion IEA
    Inferred from Electronic Annotation
    more info
     
    myelin sheath IEA
    Inferred from Electronic Annotation
    more info
     
    Preferred Names
    L-lactate dehydrogenase B chain
    Names
    LDH heart subunit
    epididymis secretory protein Li 281
    lactate dehydrogenase H chain
    renal carcinoma antigen NY-REN-46
    testicular secretory protein Li 25
    NP_001167568.1
    NP_001302466.1
    NP_002291.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_017038.1 RefSeqGene

      Range
      5001..27514
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001174097.2NP_001167568.1  L-lactate dehydrogenase B chain isoform LDHB

      See identical proteins and their annotated locations for NP_001167568.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (LDHB).
      Source sequence(s)
      AL516910, BC002362, CX867875
      Consensus CDS
      CCDS8691.1
      UniProtKB/Swiss-Prot
      P07195
      UniProtKB/TrEMBL
      Q5U077
      Related
      ENSP00000379386, OTTHUMP00000165229, ENST00000396076, OTTHUMT00000258220
      Conserved Domains (2) summary
      cd05293
      Location:20330
      LDH_1; A subgroup of L-lactate dehydrogenases
      PLN02602
      Location:4332
      PLN02602; lactate dehydrogenase
    2. NM_001315537.1NP_001302466.1  L-lactate dehydrogenase B chain isoform LDHBx

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon (nt 1114-1116), while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon (nt 1135-1137). This RefSeq represents the longer, C-terminally extended isoform, which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
      Source sequence(s)
      BC002362, BU540074
      UniProtKB/Swiss-Prot
      P07195
      Conserved Domains (2) summary
      cd05293
      Location:20330
      LDH_1; A subgroup of L-lactate dehydrogenases
      TIGR01771
      Location:26326
      L-LDH-NAD; L-lactate dehydrogenase
    3. NM_002300.7NP_002291.1  L-lactate dehydrogenase B chain isoform LDHB

      See identical proteins and their annotated locations for NP_002291.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon (nt 1114-1116), while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon (nt 1135-1137). This RefSeq represents the shorter isoform, which is localized in the cytosol. Variants 1 and 2 encode the same isoform.
      Source sequence(s)
      BC002362, BU540074
      Consensus CDS
      CCDS8691.1
      UniProtKB/Swiss-Prot
      P07195
      UniProtKB/TrEMBL
      Q5U077
      Related
      ENSP00000229319, OTTHUMP00000165228, ENST00000350669, OTTHUMT00000258219
      Conserved Domains (2) summary
      cd05293
      Location:20330
      LDH_1; A subgroup of L-lactate dehydrogenases
      PLN02602
      Location:4332
      PLN02602; lactate dehydrogenase

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 108 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p7 Primary Assembly

    Genomic

    1. NC_000012.12 Reference GRCh38.p7 Primary Assembly

      Range
      21635342..21657971 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006719074.2XP_006719137.1  

      Conserved Domains (2) summary
      cd05293
      Location:20279
      LDH_1; A subgroup of L-lactate dehydrogenases
      TIGR01771
      Location:26279
      L-LDH-NAD; L-lactate dehydrogenase

    Alternate CHM1_1.1

    Genomic

    1. NC_018923.2 Alternate CHM1_1.1

      Range
      21753524..21776056 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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