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    SPB4 putative ATP-dependent RNA helicase SPB4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850549, updated on 13-Apr-2024

    Summary

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    Gene symbol
    SPB4
    Gene description
    putative ATP-dependent RNA helicase SPB4
    Primary source
    SGD:S000001894
    Locus tag
    YFL002C
    See related
    AllianceGenome:SGD:S000001894
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Predicted to enable RNA helicase activity. Involved in assembly of large subunit precursor of preribosome; maturation of LSU-rRNA; and ribosomal large subunit assembly. Located in nucleolus and nucleoplasm. Part of 90S preribosome and preribosome, large subunit precursor. Orthologous to human DDX55 (DEAD-box helicase 55). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See SPB4 in Genome Data Viewer
    Location:
    chromosome: VI
    Exon count:
    1
    Sequence:
    Chromosome: VI; NC_001138.5 (145114..146934, complement)

    Chromosome VI - NC_001138.5Genomic Context describing neighboring genes Neighboring gene tRNA-Asn Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene pseudouridine synthase DEG1 Neighboring gene Loc1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001138.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dynamics of the Spb4 interactome monitored by affinity purification. García-Gómez JJ, et al. Methods Mol Biol, 2015. PMID 25579579
    2. Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. de la Cruz J, et al. RNA, 1998 Oct. PMID 9769101, Free PMC Article
    3. Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI. Naitou M, et al. Yeast, 1996 Jan. PMID 8789262
    4. 18S rRNA processing requires the RNA helicase-like protein Rrp3. O'Day CL, et al. Nucleic Acids Res, 1996 Aug 15. PMID 8774901, Free PMC Article
    5. Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46. Sachs AB, et al. Science, 1990 Mar 2. PMID 2408148

    See all (60) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in assembly of large subunit precursor of preribosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maturation of LSU-rRNA IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribonucleoprotein complex biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribosomal large subunit assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of 90S preribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of 90S preribosome IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in nucleolus HDA PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    putative ATP-dependent RNA helicase SPB4
    NP_116654.1
    • Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001138.5 Reference assembly

      Range
      145114..146934 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179964.1 → NP_116654.1  TPA: putative ATP-dependent RNA helicase SPB4 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_116654.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A7A237, D6VTM9, P25808
      UniProtKB/TrEMBL
      A0A8H8UL81, G2WDD8, N1P6R3
      Conserved Domains (2) summary
      COG0513
      Location:1 → 383
      SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
      pfam13959
      Location:409 → 466
      DUF4217; Domain of unknown function (DUF4217)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25808.1

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