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    tolC outer membrane channel TolC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947521, updated on 4-Apr-2024

    Summary

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    Gene symbol
    tolC
    Gene description
    outer membrane channel TolC
    Primary source
    ASAP:ABE-0009965
    Locus tag
    b3035
    See related
    ECOCYC:EG11009
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    colE1-i; ECK3026; mtcB; mukA; refI; toc; weeA
    Summary
    TolC, MdtQ, MdtP and CusC are paralogs. [More information is available at EcoGene: EG11009]. TolC is an outer membrane porin involved in the efflux of several hydrophobic and amphipathic molecules. [More information is available at EcoCyc: EG11009].
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    Genomic context

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    Sequence:
    NC_000913.3 (3178115..3179596)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1249 domain-containing protein YqiB Neighboring gene ADP-sugar pyrophosphatase Neighboring gene DUF1190 domain-containing protein YgiB Neighboring gene putative acid--amine ligase YgiC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection. Tamer YT, et al. Mol Biol Evol, 2021 Sep 27. PMID 34175926, Free PMC Article
    2. Biophysical characterization of E. coli TolC interaction with the known blocker hexaamminecobalt. Gilardi A, et al. Biochim Biophys Acta Gen Subj, 2017 Nov. PMID 28746830
    3. Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells. Pu Y, et al. Mol Cell, 2016 Apr 21. PMID 27105118, Free PMC Article
    4. Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli. Wang C, et al. Sci Rep, 2015 Nov 13. PMID 26563610, Free PMC Article
    5. Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC. Agarwal R, et al. FEBS Lett, 2014 Nov 3. PMID 25218435, Free PMC Article

    See all (220) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.
      Title: The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.
    2. In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
      Title: In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
    3. Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
      Title: Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
    4. Extreme Acid Modulates Fitness Trade-Offs of Multidrug Efflux Pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12.
      Title: Extreme Acid Modulates Fitness Trade-Offs of Multidrug Efflux Pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12.
    5. Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
      Title: Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
    6. Structural characterization of the EmrAB-TolC efflux complex from E. coli.
      Title: Structural characterization of the EmrAB-TolC efflux complex from E. coli.
    7. EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058.
      Title: EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058.
    8. Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
      Title: Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
    9. AcrA, the membrane fusion protein, connects the inner-membrane-bound AcrB to the outer-membrane-bound TolC to form the multidrug efflux pump.
      Title: Conformational Dynamics of AcrA Govern Multidrug Efflux Pump Assembly.
    10. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell.
      Title: Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer.
    Submit: New GeneRIF Correction See all GeneRIFs (52)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables bile acid transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables efflux transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables efflux transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables efflux transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables monoatomic ion channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables porin activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables porin activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within bile acid and bile salt transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within enterobactin transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in monoatomic ion transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to organic cyclic compound IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to toxic substance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to xenobiotic stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of MacAB-TolC complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of MacAB-TolC complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of efflux pump complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of efflux pump complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of efflux pump complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of efflux pump complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic side of plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    outer membrane channel TolC

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3178115..3179596
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417507.2 outer membrane channel TolC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417507.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AA86ZGL3
      Conserved Domains (1) summary
      PRK09465
      Location:1 → 451
      tolC; outer membrane channel protein; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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