unc-13 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: unc-13provided by FlyBase
Primary source: FLYBASE:FBgn0025726
Locus tag: Dmel_CG2999
See related: AllianceGenome:FB:FBgn0025726
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG2999; Dmel\CG2999; drunc13; dunc-13; dUnc-13; dUNC-13; Dunc-13; DUNC-13; Dunc13; l(4)ry16; Munc-13; Munc-13/UNC-13; unc; Unc-13; UNC-13; UNC-13-B; Unc13; unc13A; Unc13A; UNC13A; Unc13B; UNC13B
Summary: Predicted to enable calmodulin binding activity and syntaxin-1 binding activity. Involved in synaptic vesicle tethering involved in synaptic vesicle exocytosis. Located in postsynaptic membrane; presynaptic active zone; and terminal bouton. Is expressed in adult head; embryonic/larval central nervous system; neuromuscular junction; presumptive embryonic/larval nervous system; and spermatozoon. Used to study alcohol use disorder. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis. Orthologous to several human genes including UNC13A (unc-13 homolog A) and UNC13B (unc-13 homolog B). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 102E5-102F1; 4-0 cM

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	4	NC_004353.4 (867824..912331, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	4	NC_004353.3 (888450..932957, complement)

Chromosome 4 - NC_004353.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Unc13A isoform is important for phasic release and olfactory memory formation at mushroom body synapses.
Title: The Unc13A isoform is important for phasic release and olfactory memory formation at mushroom body synapses.
Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila.
Title: Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila.
RIM-binding protein couples synaptic vesicle recruitment to release sites.
Title: RIM-binding protein couples synaptic vesicle recruitment to release sites.
Study in Drosophila identify Unc13A as a direct molecular target for presynaptic homeostatic potentiation (PHP) in experiments where Unc13A was delocalized from the presynaptic active zone (AZ) scaffolds. This mutant displayed sizable synaptic transmission but completely lacked PHP and AZ-remodeling.
Title: Rapid active zone remodeling consolidates presynaptic potentiation.
Study shows that ethanol regulates presynaptic activity and sedation through presynaptic unc13 proteins in drosophila. Study proposes that reducing Dunc13 activity, genetically or pharmacologically by ethanol binding to the C1 domain of Munc13-1/Dunc13, promotes a homeostatic response that leads to ethanol tolerance.
Title: Ethanol Regulates Presynaptic Activity and Sedation through Presynaptic Unc13 Proteins in Drosophila.
Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission
Title: Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission.
identified isoform-specific Unc13-Active Zone scaffold interactions regulating Synaptic Vesicle-Ca2+-channel topology whose developmental tightening optimizes synaptic transmission.
Title: Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca(2+) channel-vesicle coupling.
Munc13-1 C1 has binding site(s) for alcohols and Munc13-1 activity is sufficient to restore normal self-administration to Drosophila mutants
Title: The pre-synaptic Munc13-1 binds alcohol and modulates alcohol self-administration in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

mobile_element

Loc: c870392-870072 mobile_element_type = transposon:INE-1{}2716
Loc: c872325-872278 mobile_element_type = transposon:INE-1{}2718
Loc: c885104-885010 mobile_element_type = transposon:INE-1{}2740
Loc: c886527-886194 mobile_element_type = transposon:INE-1{}2741
Loc: c886671-886620 mobile_element_type = transposon:INE-1{}2742
Loc: c895820-894624 mobile_element_type = transposon:Bari2{}1537
Loc: c896196-896116 mobile_element_type = transposon:INE-1{}2743
Loc: c897876-897739 mobile_element_type = transposon:INE-1{}2745
Loc: c907100-906135 mobile_element_type = transposon:transib3{}1538
Loc: c909422-909276 mobile_element_type = transposon:INE-1{}2747
Loc: c910390-909969 mobile_element_type = transposon:invader1{}1540

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	TAS Traceable Author Statement more info	PubMed
enables diacylglycerol binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables syntaxin-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-1 binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dense core granule priming	IBA Inferred from Biological aspect of Ancestor more info
involved_in neurotransmitter secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in presynaptic dense core vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle priming	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle tethering involved in synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in neuromuscular junction	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone cytoplasmic component	IBA Inferred from Biological aspect of Ancestor more info
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in terminal bouton	IBA Inferred from Biological aspect of Ancestor more info
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: unc-13

Names: CG2999-PA; CG2999-PB; CG2999-PC; CG2999-PD; CG2999-PE; CG2999-PF; CG2999-PG; lethal (4) ry16; unc-13-PA; unc-13-PB; unc-13-PC; unc-13-PD; unc-13-PE; unc-13-PF; unc-13-PG; uncoordinated-13

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004353.4 Reference assembly

Range

867824..912331 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001258497.2 → NP_001245426.1 unc-13, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245426.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8IM87

Conserved Domains (5) summary

cd04027
Location:1845 → 1971

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:2717 → 2837

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:1723 → 1772

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2162 → 2269

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2517 → 2677

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain
NM_001297788.1 → NP_001284717.1 unc-13, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001284717.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J9E1

Conserved Domains (5) summary

cd04027
Location:1845 → 1971

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:2717 → 2837

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:1723 → 1772

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2162 → 2269

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2517 → 2677

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain
NM_001272146.1 → NP_001259075.1 unc-13, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259075.1

Status: REVIEWED

UniProtKB/TrEMBL

L0MPR2

Conserved Domains (5) summary

cd08395
Location:2557 → 2677

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:1646 → 1695

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2002 → 2109

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2357 → 2517

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain

cl14603
Location:1708 → 1811

C2; C2 domain
NM_143692.4 → NP_651949.2 unc-13, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_651949.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8IM87

Related

FBpp0088305

Conserved Domains (5) summary

cd04027
Location:1845 → 1971

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:2717 → 2837

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:1723 → 1772

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2162 → 2269

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2517 → 2677

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain
NM_166793.4 → NP_726614.2 unc-13, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726614.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9V483

Conserved Domains (5) summary

cd04027
Location:1848 → 1974

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:2720 → 2840

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:1726 → 1775

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2165 → 2272

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2520 → 2680

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain
NM_166794.3 → NP_726615.1 unc-13, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726615.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8IM86

Related

FBpp0088307

Conserved Domains (5) summary

cd04027
Location:2157 → 2283

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:3029 → 3149

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:2035 → 2084

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2474 → 2581

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2829 → 2989

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain
NM_001258498.2 → NP_001245427.1 unc-13, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245427.1

Status: REVIEWED

UniProtKB/TrEMBL

H9XVP2

Conserved Domains (5) summary

cd04027
Location:2160 → 2286

C2B_Munc13; C2 domain second repeat in Munc13 (mammalian uncoordinated) proteins

cd08395
Location:3032 → 3152

C2C_Munc13; C2 domain third repeat in Munc13 (mammalian uncoordinated) proteins

cd00029
Location:2038 → 2087

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam06292
Location:2477 → 2584

DUF1041; Domain of Unknown Function (DUF1041)

pfam10540
Location:2832 → 2992

Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
H9XVP2		H9XVP2
L0MPR2		L0MPR2
O96959		O96959
O96960		O96960
Q8IM86		Q8IM86
Q8IM87		Q8IM87
Q8SX82		Q8SX82
Q8T049		Q8T049
Q9U4K9		Q9U4K9
Q9V483		Q9V483
X2J9E1		X2J9E1