PPP2R5A protein phosphatase 2 regulatory subunit B'alpha [Homo sapiens (human)] - Gene

Summary

Official Symbol: PPP2R5Aprovided by HGNC
Official Full Name: protein phosphatase 2 regulatory subunit B'alphaprovided by HGNC
Primary source: HGNC:HGNC:9309
See related: Ensembl:ENSG00000066027 MIM:601643; AllianceGenome:HGNC:9309
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: B56A; PR61A; B56alpha
Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Expression: Ubiquitous expression in fat (RPKM 41.9), lung (RPKM 33.2) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1q32.3

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (212285410..212361853)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (211531574..211606538)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (212458752..212535195)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A complex of BRCA2 and PP2A-B56 is required for DNA repair by homologous recombination.
Title: A complex of BRCA2 and PP2A-B56 is required for DNA repair by homologous recombination.
Selective PP2A Enhancement through Biased Heterotrimer Stabilization.
Title: Selective PP2A Enhancement through Biased Heterotrimer Stabilization.
Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.
Title: Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.
HIV-1 Vif Triggers Cell Cycle Arrest by Degrading Cellular PPP2R5 Phospho-regulators.
Title: HIV-1 Vif Triggers Cell Cycle Arrest by Degrading Cellular PPP2R5 Phospho-regulators.
PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.
Title: PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.
Studies indicate protein phosphatase 2, regulatory subunit B', alpha isoform protein (PPP2R5A) plays an important role in many cellular activities.
Title: PPP2R5A: A multirole protein phosphatase subunit in regulating cancer development.
Overexpression of miR-218 led to inhibition of PPP2R5A expression, whereas knockdown of miR-218 increased PPP2R5A levels. Introduction of PPP2R5A abrogated miR218-mediated cell survival and drug resistance.
Title: MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway.
A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A(B56), modulates APC/C(Cdc20) assembly.
Title: The PP2A(B56) phosphatase promotes the association of Cdc20 with APC/C in mitosis.
Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions.
Title: Expanding the PP2A Interactome by Defining a B56-Specific SLiM.
cyclin E is specifically dephosphorylated at S384 by the PP2A-B56 phosphatase, thereby uncoupling cyclin E degradation from cyclin E-CDK2 activity
Title: The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	HIV-1 Tat upregulates the total levels of PP2A protein and downregulates the inactive form of phosphorylated PP2A, which leads to inhibit hTERT activity directly or indirectly in CD4+ T cells	PubMed
	tat	Protein phosphatase-2A (PP2A) and protein phosphatase-1 (PP1) regulate HIV-1 Tat-activated transcription through dephosphorylation of CDK9	PubMed
	tat	An increase in the amount of PP2A core enzyme with a concomitant decrease in the amount of PP2A holoenzyme inhibits HIV-1 Tat-stimulated transcription from the HIV-1 LTR promoter, indicating a role for PP2A in the modulation of HIV-1 gene expression	PubMed
Vif	vif	HIV-1 Vif degrades PPP2R5A in CEM-T4 T cells	PubMed
	vif	HIV-1 Vif binds PPP2R5A expression in CEM-T4 T cells	PubMed
	vif	HIV-1 Vif downregulates PPP2R5A expression in a Cul5 dependent manner in CEM-T4 T cells	PubMed
Vpr	vpr	Amino-acid peptide sequence (residues 77-92) of HIV-1 Vpr binds to PP2A1. The sequence from HIV-1 89.6 strain is a cell penetrating and death domain	PubMed
	vpr	HIV-1 Vpr was found to upregulate PP2A in fission yeast	PubMed
	vpr	HIV-1 Vpr forms a complex with HIV-1 nucleocapsid that directly activates PP2A	PubMed
capsid	gag	The interaction of HIV-1 CA with human cellular protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A) is identified by yeast two-hybrid screen	PubMed
nucleocapsid	gag	HIV-1 Vpr forms a complex with HIV-1 nucleocapsid that directly activates PP2A	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC_15687-15688:1013522980:1 (e-PCR)
- UniSTS:267867

SHGC-76301 (e-PCR)
- UniSTS:24569

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein phosphatase regulator activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of lipid kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in M band	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein phosphatase type 2A complex	IBA Inferred from Biological aspect of Ancestor more info
part_of protein phosphatase type 2A complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Names: PR61alpha; protein phosphatase 2 regulatory subunit B', alpha

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199756.2 → NP_001186685.1 serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2

See identical proteins and their annotated locations for NP_001186685.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.

Source sequence(s)

AK302202, BP326450, L42373

Consensus CDS

CCDS55686.1

UniProtKB/Swiss-Prot

Q15172

Related

ENSP00000442866.1, ENST00000537030.3

Conserved Domains (1) summary

pfam01603
Location:7 → 385

B56; Protein phosphatase 2A regulatory B subunit (B56 family)
NM_006243.4 → NP_006234.1 serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1

See identical proteins and their annotated locations for NP_006234.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AL451060, L42373

Consensus CDS

CCDS1503.1

UniProtKB/Swiss-Prot

B2R6D2, B7Z7L2, D3DT99, Q15172, Q2NL72, Q5VVB2, Q8TBI9

Related

ENSP00000261461.2, ENST00000261461.7

Conserved Domains (1) summary

pfam01603
Location:64 → 442

B56; Protein phosphatase 2A regulatory B subunit (B56 family)