CDC34 cell division cycle 34 homolog (S. cerevisiae) [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: CDC34provided by HGNC
Official Full Name: cell division cycle 34 homolog (S. cerevisiae)provided by HGNC
Primary source: HGNC:1734
See related: Ensembl:ENSG00000099804; HPRD:00306; MIM:116948
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: UBC3; UBCH3; UBE2R1; E2-CDC34
Summary: The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]

Help top of page

Genomic context

Location :: 19p13.3

Sequence :: Chromosome: 19; NC_000019.9 (531733..542087)

See CDC34 in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc34 phosphorylation by PKA and Sch9 provides a direct tether between G1 cell division events and cell growth
Title: Nutrient sensing kinases PKA and Sch9 phosphorylate the catalytic domain of the ubiquitin-conjugating enzyme Cdc34.
Data show that anaphase-promoting complex or cyclosome (APC/C)-mediated multiple monoubiquitylation targeting cyclin B1 for degradation.
Title: APC/C-mediated multiple monoubiquitylation provides an alternative degradation signal for cyclin B1.
work provides the first structural details that show how the C-terminus of CDC34 might direct a thiolester-bound Ub to control polyubiquitin chain formation
Title: Association of the disordered C-terminus of CDC34 with a catalytically bound ubiquitin.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
the human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination
Title: The human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination.
COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation
Title: The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
determined that a ubiquitin (Ub) fused at I kappaB alpha K21 acts as a receptor, directing Cdc34 for rapid and efficient K48-linked Ub chain synthesis that depends on SCF(beta TrCP2) and the substrate's N terminus.
Title: Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate.
The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4.
Title: The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4.
Study shows that chain assembly by ubiquitin ligase SCF and ubiquitin-conjugating enzyme Cdc34 is facilitated by the unusual nature of Cdc34-SCF transactions: Cdc34 binds SCF with nanomolar affinity, nevertheless the complex is extremely dynamic.
Title: Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates.
Cdc34 is a functional target of let-7 and that let-7 induces down-regulation of Cdc34, stabilization of the Wee1 kinase, and an increased fraction of cells in G(2)/M in primary fibroblasts.
Title: let-7 Overexpression leads to an increased fraction of cells in G2/M, direct down-regulation of Cdc34, and stabilization of Wee1 kinase in primary fibroblasts.

Submit: New GeneRIF Correction See all (19)

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
P49427	Q9Y2D1	ATF5	HPRD	PubMed
P49427	Q9Y297	BTRC	HPRD	PubMed
P49427	P49427	CDC34	HPRD	PubMed
P49427	P50750	CDK9	HPRD	PubMed
P49427	Q03060	CREM	HPRD	PubMed
P49427	P67870	CSNK2B	HPRD	PubMed
P49427	Q13616	CUL1	HPRD	PubMed
P49427	P10244	MYBL2	HPRD	PubMed
P49427	P15172	MYOD1	HPRD	PubMed
P49427	Q15843	NEDD8	HPRD	PubMed
P49427	P25963	NFKBIA	HPRD	PubMed
P49427	Q13464	ROCK1	HPRD	PubMed
P49427	Q13309	SKP2	HPRD	PubMed
P49427	Ubiquitin B	UBB	HPRD	PubMed
P49427	Q6FIF0	ZFAND6	HPRD	PubMed
BioGRID:107432	BioGRID:116487	ATF5	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107432	BioGRID:114457	BTRC	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:107432	BioGRID:107459	CDK9	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:107432	BioGRID:107461	CDKN1B	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:122728	CORO7	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107432	BioGRID:107843	CSNK2B	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:107432	BioGRID:114032	CUL1	BioGRID	PubMed	Reconstituted Complex
BioGRID:107432	BioGRID:117615	FBXL3	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:117656	FBXO4	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:117326	FBXO7	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:117603	FBXW2	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:110859	NFKBIA	BioGRID	PubMed	Biochemical Activity
BioGRID:107432	BioGRID:111310	PIM1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107432	BioGRID:111822	RAD21	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:107432	BioGRID:115301	RBX1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:107432	BioGRID:971426	RL2	BioGRID	PubMed	Biochemical Activity; Reconstituted Complex
BioGRID:107432	BioGRID:112393	SKP2	BioGRID	PubMed	Reconstituted Complex
BioGRID:107432	BioGRID:112497	SUMO2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107432	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Reconstituted Complex

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
acid-amino acid ligase activity	IEA Inferred from Electronic Annotation more info
ligase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
ubiquitin-protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
ubiquitin-protein ligase activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
DNA-dependent DNA replication initiation	NAS Non-traceable Author Statement more info	PubMed
G1/S transition of mitotic cell cycle	NAS Non-traceable Author Statement more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
negative regulation of cAMP-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info
protein modification process	NAS Non-traceable Author Statement more info	PubMed
protein polyubiquitination	IDA Inferred from Direct Assay more info
protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
protein ubiquitination	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
NOT nucleolus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

Preferred Names: ubiquitin-conjugating enzyme E2 R1

Names: ubiquitin-conjugating enzyme E2 R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing

NP_004350.1

EC 6.3.2.19

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_004359.1 → NP_004350.1 ubiquitin-conjugating enzyme E2 R1

Status: REVIEWED

Source sequence(s)

BC009850

Consensus CDS

CCDS12030.1

UniProtKB/Swiss-Prot

P49427

Related

ENSP00000215574, ENST00000215574

Conserved Domains (1) summary

cd00195
Location:11 – 162
Blast Score: 423

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p5 Primary Assembly

Range

531733..542087

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

302243..312073

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC011531.7 (21220..29376)	None
genomic	AC011556.9 (8370..10567)	None
genomic	AY650399.1	AAT46688.1
genomic	CH471242.1	EAW61190.1
		EAW61191.1
mRNA	AK291554.1	BAF84243.1
mRNA	BC002332.2	None
mRNA	BC009850.2	AAH09850.1
mRNA	BC018143.2	AAH18143.1
mRNA	BC023979.1	AAH23979.1
mRNA	BT006659.1	AAP35305.1
mRNA	L22005.1	AAC37534.1
other-genetic	DQ890738.2	ABM81664.1
other-genetic	DQ893910.2	ABM84836.1
other-genetic	DQ894652.2	ABM85578.1