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    EIF5B eukaryotic translation initiation factor 5B [ Homo sapiens ]

    Gene ID: 9669, updated on 12-May-2012
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    Summary

    Official Symbol
    EIF5Bprovided by HGNC
    Official Full Name
    eukaryotic translation initiation factor 5Bprovided by HGNC
    Primary source
    HGNC:30793
    See related
    Ensembl:ENSG00000158417; HPRD:07304; MIM:606086; Vega:OTTHUMG00000153242
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    IF2; FLJ10524; KIAA0741; DKFZp434I036
    Summary
    Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
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    Genomic context

    Location :
    2q11.2
    Sequence :
    Chromosome: 2; NC_000002.11 (99953834..100016728)
    See EIF5B in Epigenomics, MapViewer

    Chromosome 2 - NC_000002.11Genomic Context describing neighboring genes Neighboring gene lysozyme G-like 1 Neighboring gene thioredoxin domain containing 9 Neighboring gene REV1, polymerase (DNA directed) Neighboring gene AF4/FMR2 family, member 3

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Identification of ubiquitinated proteins from human multiple myeloma U266 cells by proteomics. Jia H, et al. Biomed Environ Sci, 2011 Aug. PMID 22108332.
    2. Interactome study suggests multiple cellular functions of hepatoma-derived growth factor (HDGF). Zhao J, et al. J Proteomics, 2011 Dec 21. PMID 21907836.
    3. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, et al. Mol Cell Proteomics, 2011 Oct. PMID 21890473.
    4. Poliovirus switches to an eIF2-independent mode of translation during infection. White JP, et al. J Virol, 2011 Sep. PMID 21697471.
    5. Transcriptional regulation of Oct4 by a long non-coding RNA antisense to Oct4-pseudogene 5. Hawkins PG, et al. Transcription, 2010 Nov. PMID 21151833.

    See all (25) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. The authors show that the cleavage of initiation factor eIF5B during enteroviral infection, along with the viral internal ribosome entry site, plays a role in mediating viral translation under conditions that are nonpermissive for host cell translation.
      Title: Poliovirus switches to an eIF2-independent mode of translation during infection.
    2. 3Cpro-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells.
      Title: Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases.
    3. binding of eIF5B might induce conformational changes in both subunits, with ribosomal segments wrapping around the factor
      Title: Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probing.
    4. Transfected nuclear factor 2 trans activates the IF2 promoter in a cell line.
      Title: Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene.
    5. determination of binding site on eukaryotic initiation factor 1A
      Title: Mapping the binding interface between human eukaryotic initiation factors 1A and 5B: a new interaction between old partners.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    HIV-1 protein interactions

    Protein Gene Interaction Pubs
    matrix gag HIV-1 Matrix inhibits hIF2 mediated translation in a reticulocyte lysate assay PubMed
    gag HIV-1 Matrix, as well as the full length HIV-1 Gag protein bind to human translation initiation factor two (hIF2) and these proteins can be immunoprecipitated as a protein complex PubMed

    Go to the HIV-1, Human Protein Interaction Database

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_056988.2 NP_579876.2 gag    BIND  PubMed hIF2 interacts with p17. 
    O60841 P22681 CBL    HPRD  PubMed  
    O60841 P47813 EIF1AX    HPRD  PubMed  
    BioGRID:115024 BioGRID:109318 HDGF    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115024 BioGRID:204968 Mapk13    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115024 BioGRID:111456 POU5F1    BioGRID  PubMed Affinity Capture-RNA 
    BioGRID:115024 BioGRID:111549 PRKAA1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115024 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115024 BioGRID:122933 ZBTB3    BioGRID  PubMed Two-hybrid 
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    General gene information

    Markers

    Genotypes

    Homology

    • Homologs of the EIF5B gene: The EIF5B gene is conserved in chimpanzee, , dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, , S.pombe, , N.crassa, A.thaliana, rice, and P.falciparum.
    • Map Viewer (Mouse, Rat)

    Pathways from BioSystems

    • Cap-dependent Translation Initiation, organism-specific biosystem (from REACTOME)
      Cap-dependent Translation Initiation, organism-specific biosystemTranslation initiation is a complex process in which the Met-tRNAi initiator, 40S, and 60S ribosomal subunits are assembled by eukaryotic initiation factors (eIFs) into an 80S ribosome at the start c...
    • Eukaryotic Translation Initiation, organism-specific biosystem (from REACTOME)
      Eukaryotic Translation Initiation, organism-specific biosystemInitiation of translation in the majority of eukaryotic cellular mRNAs depends on the 5'-cap (m7GpppN) and involves ribosomal scanning of the 5' untranslated region (5'-UTR) for an initiating AUG sta...
    • GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem (from REACTOME)
      GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystemHydrolysis of eIF2-GTP occurs after the Met-tRNAi has recognized the AUG. This reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow ...
    • Gene Expression, organism-specific biosystem (from REACTOME)
      Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
    • Metabolism of proteins, organism-specific biosystem (from REACTOME)
      Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
    • RNA transport, organism-specific biosystem (from KEGG)
      RNA transport, organism-specific biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
    • RNA transport, conserved biosystem (from KEGG)
      RNA transport, conserved biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
    • Translation, organism-specific biosystem (from REACTOME)
      Translation, organism-specific biosystemProtein synthesis is accomplished through the process of translation of an mRNA sequence into a polypeptide chain. This process can be divided into three distinct stages: initiation, elongation and ...
    • Translation Factors, organism-specific biosystem (from WikiPathways)
      Translation Factors, organism-specific biosystemProtein synthesis is the ultimate step of gene expression and a key control point for regulation. In particular, it enables cells to rapidly manipulate protein production without new mRNA synthesis, ...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    translation initiation factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    translation initiation factor activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    cellular protein metabolic process TAS
    Traceable Author Statement
    more info
    		  
    gene expression TAS
    Traceable Author Statement
    more info
    		  
    regulation of translational initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    regulation of translational initiation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    translation TAS
    Traceable Author Statement
    more info
    		  
    translational initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    translational initiation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    translational initiation TAS
    Traceable Author Statement
    more info
    		  
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    cytosol TAS
    Traceable Author Statement
    more info
    		  
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    General protein information

    Preferred Names
    eukaryotic translation initiation factor 5B
    Names
    eukaryotic translation initiation factor 5B
    eIF-5B
    translation initiation factor IF2
    translation initiation factor IF-2
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_015904.3 → NP_056988.3  eukaryotic translation initiation factor 5B

      Status: REVIEWED

      Source sequence(s)
      AB018284, AC018690, BC032639, BX485839
      Consensus CDS
      CCDS42721.1
      UniProtKB/Swiss-Prot
      O60841
      UniProtKB/TrEMBL
      Q8N5A0
      Related
      ENSP00000289371, OTTHUMP00000203504, ENST00000289371, OTTHUMT00000330364
      Conserved Domains (4) summary
      cd03703
      Location:856 – 965
      Blast Score: 393
      aeIF5B_II; aeIF5B_II: This family represents the domain II of archeal and eukaryotic aeIF5B. aeIF5B is a homologue of prokaryotic Initiation Factor 2 (IF2). Disruption of the eIF5B gene (FUN12) in yeast causes a severe slow-growth phenotype, associated with a ...
      PRK04004
      Location:627 – 1202
      Blast Score: 1432
      PRK04004; translation initiation factor IF-2; Validated
      pfam11987
      Location:964 – 1067
      Blast Score: 194
      IF-2; Translation-initiation factor 2
      cd01887
      Location:632 – 845
      Blast Score: 633
      IF2_eIF5B; Initiation Factor 2 (IF2)/ eukaryotic Initiation Factor 5B (eIF5B) family

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000002.11 Reference GRCh37.p5 Primary Assembly

      Range
      99953834..100016728
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000134.1 Alternate HuRef

      Range
      93719194..93782572
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC018690.5 AAY24313.1
    genomic AC079447.4 AAX93258.1
    genomic CH471127.2 EAX01864.1
      EAX01865.1
      EAX01866.1
      EAX01867.1
    mRNA AB018284.1 BAA34461.2
    mRNA AF078035.1 AAD16006.1
    mRNA AJ006412.1 CAA07018.1
    mRNA AJ006776.1 CAB44357.1
    mRNA AK001386.1 BAG50898.1
    mRNA AK308175.1 None
    mRNA AK308176.1 None
    mRNA AL133563.1 CAB63717.1
    mRNA BC006970.1 AAH06970.1
    mRNA BC022260.1 AAH22260.1
    mRNA BC032639.1 AAH32639.1
    mRNA BX485839.1 None
    Protein Accession Links
    GenPept Link UniProtKB Link
    O60841.4 GenPept UniProtKB/Swiss-Prot:O60841
    Q05CQ1 GenPept UniProtKB/TrEMBL:Q05CQ1
    Q8N5A0 GenPept UniProtKB/TrEMBL:Q8N5A0
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    Additional Links

    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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