PPM1F protein phosphatase, Mg2+/Mn2+ dependent, 1F [Homo sapiens] - Gene

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Summary

Official Symbol: PPM1Fprovided by HGNC
Official Full Name: protein phosphatase, Mg2+/Mn2+ dependent, 1Fprovided by HGNC
Primary source: HGNC:19388
See related: Ensembl:ENSG00000100034; HPRD:10163; Vega:OTTHUMG00000150835
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase; KIAA0015
Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 22q11.22

Sequence :: Chromosome: 22; NC_000022.10 (22273792..22307250, complement)

See PPM1F in Epigenomics, MapViewer

Chromosome 22 - NC_000022.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples.
Title: MicroRNA-200c represses migration and invasion of breast cancer cells by targeting actin-regulatory proteins FHOD1 and PPM1F.
Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility.
Title: Investigation of POPX2 phosphatase functions by comparative phosphoproteomic analysis.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
The POPX2 phosphatase regulates cancer cell motility and invasiveness.
Title: The POPX2 phosphatase regulates cancer cell motility and invasiveness.
PPM1F plays a key role in the regulation of CaMKII intracellularly
Title: Regulation of the multifunctional Ca2+/calmodulin-dependent protein kinase II by the PP2C phosphatase PPM1F in fibroblasts.
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2)
Title: The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Molecular genetics of adult ADHD: converging evidence from genome-wide association and extended pedigree linkage studies.

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HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Microarray analysis indicates HIV-1 Tat downregulates the expression of many proteins, including protein phosphatase 1F (KIAA0015), in immature dendritic cells, an effect that likely facilitates the expansion of HIV-1 infection	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
P49593	Q14155	ARHGEF7	HPRD	PubMed
P49593	Q9UQM7	CAMK2A	HPRD	PubMed
P49593	FEM1A	FEM1A	HPRD	PubMed
P49593	P56192	MARS	HPRD	PubMed
P49593	Q13153	PAK1	HPRD	PubMed
P49593	P49593	PPM1F	HPRD	PubMed
BioGRID:115005	BioGRID:114393	ARHGEF7	BioGRID	PubMed	Reconstituted Complex
BioGRID:115005	BioGRID:123909	FAM161A	BioGRID	PubMed	Two-hybrid
BioGRID:115005	BioGRID:111657	PSMA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:115005	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
calmodulin-dependent protein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
hydrolase activity	IEA Inferred from Electronic Annotation more info
metal ion binding	IEA Inferred from Electronic Annotation more info
phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein serine/threonine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
protein serine/threonine phosphatase activity	IMP Inferred from Mutant Phenotype more info

Process	Evidence Code	Pubs
cellular response to drug	IDA Inferred from Direct Assay more info
intrinsic apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
negative regulation of peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
negative regulation of protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
negative regulation of protein kinase activity by regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
negative regulation of transcription, DNA-dependent	IMP Inferred from Mutant Phenotype more info
peptidyl-threonine dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
positive regulation of cell-substrate adhesion	IMP Inferred from Mutant Phenotype more info
positive regulation of chemotaxis	IMP Inferred from Mutant Phenotype more info
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	IDA Inferred from Direct Assay more info	PubMed
positive regulation of epithelial cell migration	IMP Inferred from Mutant Phenotype more info
positive regulation of focal adhesion assembly	IMP Inferred from Mutant Phenotype more info
positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info
positive regulation of growth	IMP Inferred from Mutant Phenotype more info
positive regulation of stress fiber assembly	IDA Inferred from Direct Assay more info	PubMed
positive regulation of stress fiber assembly	IMP Inferred from Mutant Phenotype more info

Component	Evidence Code	Pubs
cytosol	IDA Inferred from Direct Assay more info	PubMed
protein complex	IDA Inferred from Direct Assay more info	PubMed
protein serine/threonine phosphatase complex	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: protein phosphatase 1F

Names: protein phosphatase 1F; PP2C phosphatase; partner of PIX 2; protein fem-2 homolog; CaM-kinase phosphatase; protein phosphatase 1F (PP2C domain containing); Ca(2+)/calmodulin-dependent protein kinase phosphatase

NP_055449.1

EC 3.1.3.16

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_014634.3 → NP_055449.1 protein phosphatase 1F

Status: REVIEWED

Source sequence(s)

AK291628, AP000555, DA311634, N34773

Consensus CDS

CCDS13796.1

UniProtKB/Swiss-Prot

P49593

Related

ENSP00000263212, OTTHUMP00000198593, ENST00000263212, OTTHUMT00000320267

Conserved Domains (2) summary

cd00143
Location:155 – 413
Blast Score: 552

PP2Cc; Serine/threonine phosphatases, family 2C, catalytic domain; The protein architecture and deduced catalytic mechanism of PP2C phosphatases are similar to the PP1, PP2A, PP2B family of protein Ser/Thr phosphatases, with which PP2C shares no sequence ...

pfam00481
Location:155 – 406
Blast Score: 621

PP2C; Protein phosphatase 2C

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000022.10 Reference GRCh37.p5 Primary Assembly

Range

22273792..22307250, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000154.1 Alternate HuRef

Range

5230870..5264327, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AP000555.1 (157944..168812)	None
genomic	CH471095.1	EAW59483.1
		EAW59484.1
		EAW59485.1
genomic	D86995.1	BAA19990.1
mRNA	AF305840.1	AAL15579.1
mRNA	AF520615.1	AAM76059.1
mRNA	AK126377.1	BAG54318.1
mRNA	AK291628.1	BAF84317.1
mRNA	AK294557.1	BAH11809.1
mRNA	AU127806.1	None
mRNA	BC071989.1	AAH71989.1
mRNA	BC105599.1	AAI05600.1
mRNA	CA425300.1	None
mRNA	D13640.1	BAA02803.2
mRNA	DA311634.1	None
mRNA	N34773.1	None
other-genetic	EU831437.1	ACE87124.1
other-genetic	EU831529.1	ACE86448.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P49593.3	GenPept	UniProtKB/Swiss-Prot:P49593
Q0VGL7	GenPept	UniProtKB/TrEMBL:Q0VGL7
Q6IPC0	GenPept	UniProtKB/TrEMBL:Q6IPC0