CD8B CD8b molecule [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: CD8Bprovided by HGNC
Official Full Name: CD8b moleculeprovided by HGNC
Primary source: HGNC:1707
See related: Ensembl:ENSG00000172116; HPRD:01725; MIM:186730; Vega:OTTHUMG00000130264
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: LY3; P37; LEU2; LYT3; CD8B1; MGC119115
Summary: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

Help top of page

Genomic context

Location :: 2p12

Sequence :: Chromosome: 2; NC_000002.11 (87042460..87089047, complement)

See CD8B in Epigenomics, MapViewer

Chromosome 2 - NC_000002.11 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nef-mediated down-modulation of CD8alphabeta is a fundamental property of primate lentiviruses.
Title: Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.
[review] Coreceptor CD8alphabeta contributes to the antigen-recognition process by binding to a largely invariant region of the MHCI molecule and by promoting intracellular signaling.
Title: The multiple roles of the CD8 coreceptor in T cell biology: opportunities for the selective modulation of self-reactive cytotoxic T cells.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Data show that CD8+LAP+ cells produce IFN-gamma, and these cells suppress EAE that is dependent on both TGF-beta and IFN-gamma.
Title: Novel CD8+ Treg suppress EAE by TGF-beta- and IFN-gamma-dependent mechanisms.
study demonstrated differential mRNA expression patterns of CD8beta splice variants in thymocytes and in resting, memory, and activated primary CD8(+) T cells
Title: Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination.
RT-PCR, and related methodologies are not useful substitutes for assessment of CD4 and CD8 cell numbers in HIV-infected persons.
Title: Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection.
A clustering of DNase hypersensitivity sites and matrix attachment regions capable of binding SATB1 and GATA-3, colocalized at the 3' end of the CD8B gene, suggests that this region is an epigenetic regulator of CD8 expression.
Title: Identification of a candidate regulatory region in the human CD8 gene complex by colocalization of DNase I hypersensitive sites and matrix attachment regions which bind SATB1 and GATA-3.

Submit: New GeneRIF Correction

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Identification of common variants influencing risk of the tauopathy progressive supranuclear palsy.

Help top of page

HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Nef	nef	Downregulation of CD8beta by HIV-1 Nef requires an intact AP-2 complex	PubMed
	nef	Co-localization of HIV-1 Nef and CD8beta is revealed by staining assays in 293T cells expressing both proteins	PubMed
	nef	HIV-1 Nef downregulates surface expression of the beta-chain of the CD8 receptor by accelerated endocytosis; amino acid positions 57 to 58 and 174 to 175 in Nef are involved in the downmodulation	PubMed

Go to the HIV-1, Human Protein Interaction Database

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
P10966	P04234	CD3D	HPRD	PubMed
P10966	P01732	CD8A	HPRD	PubMed
P10966	P10966	CD8B	HPRD	PubMed
P10966	Q11206	ST3GAL4	HPRD	PubMed
BioGRID:107364	BioGRID:107363	CD8A	BioGRID	PubMed	Co-localization
	NP_005347.2	LCK	BIND	PubMed	CD8-Beta interacts with Lck.

Function	Evidence Code	Pubs
MHC class I protein binding	NAS Non-traceable Author Statement more info	PubMed
coreceptor activity	NAS Non-traceable Author Statement more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
T cell activation	NAS Non-traceable Author Statement more info	PubMed
immune response	NAS Non-traceable Author Statement more info	PubMed
regulation of defense response to virus by virus	TAS Traceable Author Statement more info
regulation of immune response	TAS Traceable Author Statement more info
transmembrane receptor protein tyrosine kinase signaling pathway	NAS Non-traceable Author Statement more info	PubMed
viral reproduction	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
T cell receptor complex	NAS Non-traceable Author Statement more info	PubMed
early endosome	TAS Traceable Author Statement more info
extracellular region	IEA Inferred from Electronic Annotation more info
integral to plasma membrane	TAS Traceable Author Statement more info	PubMed
plasma membrane	TAS Traceable Author Statement more info

General protein information

Preferred Names: T-cell surface glycoprotein CD8 beta chain

Names: T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001178100.1 → NP_001171571.1 T-cell surface glycoprotein CD8 beta chain isoform 6 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (6) lacks two exons in the 3' coding region, which results in a frameshift, compared to variant 2. The resulting protein (isoform 6) is shorter and has a distinct C-terminus compared to isoform 2.

Source sequence(s)

AW296309, BC100912, DC405359, X13445

Consensus CDS

CCDS54376.1

UniProtKB/Swiss-Prot

P10966

UniProtKB/TrEMBL

Q496E2

Related

ENSP00000377358, ENST00000393761

Conserved Domains (2) summary

cd07700
Location:29 – 134
Blast Score: 461

IgV_CD8_beta; Immunoglobulin (Ig) like domain of CD8 beta chain

pfam07686
Location:20 – 134
Blast Score: 223

V-set; Immunoglobulin V-set domain
NM_004931.4 → NP_004922.1 T-cell surface glycoprotein CD8 beta chain isoform 5 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (5), also known as M-1, differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 2.

Source sequence(s)

AC111200, BC100912, BP392114, DB223179, DC405359, X13444

Consensus CDS

CCDS1997.1

UniProtKB/Swiss-Prot

P10966

UniProtKB/TrEMBL

Q496E2

Related

ENSP00000375070, OTTHUMP00000203521, ENST00000390655, OTTHUMT00000330402

Conserved Domains (2) summary

cd07700
Location:29 – 134
Blast Score: 460

IgV_CD8_beta; Immunoglobulin (Ig) like domain of CD8 beta chain

pfam07686
Location:20 – 134
Blast Score: 223

V-set; Immunoglobulin V-set domain
NM_172101.3 → NP_742099.1 T-cell surface glycoprotein CD8 beta chain isoform 3 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (3), also known as M-3, includes an alternate exon in the 3' coding region, which results in a frameshift compared to variant 2. The resulting membrane associated protein (isoform 3) is shorter and has a distinct C-terminus, compared to isoform 2.

Source sequence(s)

AW296309, BC100912, DC405359, X13445

Consensus CDS

CCDS42708.1

UniProtKB/Swiss-Prot

P10966

UniProtKB/TrEMBL

Q496E2

Related

ENSP00000377356, OTTHUMP00000160763, ENST00000393759, OTTHUMT00000252595

Conserved Domains (2) summary

cd07700
Location:29 – 134
Blast Score: 461

IgV_CD8_beta; Immunoglobulin (Ig) like domain of CD8 beta chain

pfam07686
Location:20 – 134
Blast Score: 225

V-set; Immunoglobulin V-set domain
NM_172102.3 → NP_742100.1 T-cell surface glycoprotein CD8 beta chain isoform 4 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (4), also known as S-1, lacks an in-frame exon in the coding region, compared to variant 2. The resulting secreted protein (isoform 4) is shorter than isoform 2.

Source sequence(s)

AW296309, BC100912, DC405359, X13446

Consensus CDS

CCDS1994.1

UniProtKB/Swiss-Prot

P10966

UniProtKB/TrEMBL

Q496E2

Related

ENSP00000340592, OTTHUMP00000160762, ENST00000349455, OTTHUMT00000252594

Conserved Domains (2) summary

cd07700
Location:29 – 134
Blast Score: 456

IgV_CD8_beta; Immunoglobulin (Ig) like domain of CD8 beta chain

pfam07686
Location:20 – 134
Blast Score: 221

V-set; Immunoglobulin V-set domain
NM_172213.3 → NP_757362.1 T-cell surface glycoprotein CD8 beta chain isoform 2 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) encodes the longest isoform (2).

Source sequence(s)

AW296309, BC100912, BC100913, DC405359

Consensus CDS

CCDS1995.1

UniProtKB/Swiss-Prot

P10966

UniProtKB/TrEMBL

Q496E2

Related

ENSP00000331172, OTTHUMP00000198673, ENST00000331469, OTTHUMT00000320462

Conserved Domains (2) summary

cd07700
Location:29 – 134
Blast Score: 456

IgV_CD8_beta; Immunoglobulin (Ig) like domain of CD8 beta chain

pfam07686
Location:20 – 134
Blast Score: 218

V-set; Immunoglobulin V-set domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000002.11 Reference GRCh37.p5 Primary Assembly

Range

87042460..87089047, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000134.1 Alternate HuRef

Range

100787546..100791415

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_172099.2: Suppressed sequence

Description

NM_172099.2: This RefSeq was temporarily suppressed because currently there is not sufficient data to support this transcript.

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC111200.7	AAY24019.1
genomic	AC112696.4 (8994..27544)	None
genomic	AY032722.1	AAK91556.1
		AAK91557.1
genomic	JA738613.1	CCF76959.1
genomic	JA738615.1	CCF76960.1
genomic	JA738617.1	CCF76961.1
genomic	JA738619.1	CCF76962.1
genomic	M17514.1	AAA52283.1
genomic	S55731.1	AAD13877.1
genomic	S87075.1	AAB21668.2
genomic	S87083.1	AAB21671.2
		AAB21672.2
genomic	S87087.1	AAB21670.2
genomic	S87090.1	AAB21669.2
mRNA	AK303360.1	BAG64420.1
mRNA	AK303595.1	BAG64610.1
mRNA	AW296309.1	None
mRNA	AW611750.1	None
mRNA	BC100911.1	AAI00912.1
mRNA	BC100912.2	AAI00913.1
mRNA	BC100913.1	AAI00914.1
mRNA	BC100914.1	AAI00915.1
mRNA	BP392114.1	None
mRNA	BQ004743.1	None
mRNA	DB223179.1	None
mRNA	DC405359.1	None
mRNA	M36712.1	AAA35664.1
mRNA	X13444.1	CAA31795.1
mRNA	X13445.1	CAA31796.1
mRNA	X13446.1	CAA31797.1
mRNA	X13452.1	CAA31803.1
mRNA	Y00805.1	CAA68750.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P10966.1	GenPept	UniProtKB/Swiss-Prot:P10966
Q496E2	GenPept	UniProtKB/TrEMBL:Q496E2
Q53QL8	GenPept	UniProtKB/TrEMBL:Q53QL8
Q8TD28	GenPept	UniProtKB/TrEMBL:Q8TD28
Q8TD29	GenPept	UniProtKB/TrEMBL:Q8TD29
Q9UQ56	GenPept	UniProtKB/TrEMBL:Q9UQ56