CD8A CD8a molecule [Homo sapiens] - Gene

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Summary

Official Symbol: CD8Aprovided by HGNC
Official Full Name: CD8a moleculeprovided by HGNC
Primary source: HGNC:1706
See related: Ensembl:ENSG00000153563; HPRD:01737; MIM:186910; Vega:OTTHUMG00000130265
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CD8; MAL; p32; Leu2
Summary: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

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Genomic context

Location :: 2p12

Sequence :: Chromosome: 2; NC_000002.11 (87011728..87035519, complement)

See CD8A in Epigenomics, MapViewer

Chromosome 2 - NC_000002.11 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing Hsp25/Hsp27 gene expression augments proteasome activity and increases CD8+ T-cell-mediated tumor killing and memory responses
Title: Silencing Hsp25/Hsp27 gene expression augments proteasome activity and increases CD8+ T-cell-mediated tumor killing and memory responses.
LNX1 and LNX2 interact with CD8alpha and promote its ubiquitylation and endocytosis.
Title: Ligand of Numb proteins LNX1p80 and LNX2 interact with the human glycoprotein CD8α and promote its ubiquitylation and endocytosis.
Nef-mediated down-modulation of CD8alphabeta is a fundamental property of primate lentiviruses.
Title: Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.
Multiple sclerosis is characterized by a dysregulation of CD8+CD56-perforin+ T cells that may play a role in the development of disability.
Title: Circulating CD8+CD56-perforin+ T cells are increased in multiple sclerosis patients.
Lymphoid tissue (LT)-resident CD8alpha-positive dendritic cells (DCs) and non(LT)-derived CD103-positive DCs express transgenic XCR1 and are characterized by a unique transcriptional fingerprint, irrespective of their tissue of origin.
Title: Cutting edge: expression of XCR1 defines mouse lymphoid-tissue resident and migratory dendritic cells of the CD8α+ type.
Coreceptor CD8alphabeta contributes to the antigen-recognition process by binding to a largely invariant region of the MHC class I molecule and by promoting intracellular signaling.
Title: The multiple roles of the CD8 coreceptor in T cell biology: opportunities for the selective modulation of self-reactive cytotoxic T cells.
These results suggest that the elevated frequency of PD-1(+) CD8(+) T cells could be associated with leukemic relapse in pediatric umbilical cord blood transplantation recipients.
Title: CD8(+) T-cell reconstitution in recipients of umbilical cord blood transplantation and characteristics associated with leukemic relapse.
The aim was to determine if SPARC, FOXP3, CD8 and CD45RO expression levels are associated with colorectal cancer (CRC) stage, disease outcome and long-term cancer-specific survival
Title: SPARC, FOXP3, CD8 and CD45 correlation with disease recurrence and long-term disease-free survival in colorectal cancer.
we present the complex structures of human CD8alphaalpha bound to HLA-A*2402
Title: Plasticity of human CD8αα binding to peptide-HLA-A*2402.
the presence of IL-1 and/or IL-6 during activation of human CD8 T cells attenuates Fas-mediated AICD, whereas IL-12 increases the susceptibility of activated CD8 T cells to this form of cell death
Title: Inflammatory cytokines determine the susceptibility of human CD8 T cells to Fas-mediated activation-induced cell death through modulation of FasL and c-FLIP(S) expression.

Submit: New GeneRIF Correction See all (44)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Nef	nef	HIV-inoculated and HIV-1 Nef-treated human peripheral blood lymphocytes downregulate cell surface CD8, and this down-modulation could inhibit anti-HIV cytotoxicity associated with cytotoxic T-cell function	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
P01732	P61769	B2M	HPRD	PubMed
P01732	P04234	CD3D	HPRD	PubMed
P01732	P01732	CD8A	HPRD	PubMed
P01732	P10966	CD8B	HPRD	PubMed
P01732	P01891	HLA-A	HPRD	PubMed
P01732	P01889	HLA-B	HPRD	PubMed
P01732	Q95604	HLA-C	HPRD	PubMed
P01732	P13747	HLA-E	HPRD	PubMed
P01732	P17693	HLA-G	HPRD	PubMed
P01732	O43561	LAT	HPRD	PubMed
P01732	P06239	LCK	HPRD	PubMed
P01732	P09382	LGALS1	HPRD	PubMed
P01732	P08575	PTPRC	HPRD	PubMed
P01732	Q8WXA9	SREK1	HPRD	PubMed
P01732	Q07955	SRSF1	HPRD	PubMed
BioGRID:107363	BioGRID:107353	CD3D	BioGRID	PubMed	Affinity Capture-Western; FRET
BioGRID:107363	BioGRID:107404	CD59	BioGRID	PubMed	Reconstituted Complex
BioGRID:107363	BioGRID:107363	CD8A	BioGRID	PubMed	Co-crystal Structure; Co-localization
BioGRID:107363	BioGRID:107364	CD8B	BioGRID	PubMed	Co-localization
BioGRID:107363	BioGRID:109350	HLA-A	BioGRID	PubMed	Co-crystal Structure; Reconstituted Complex
BioGRID:107363	BioGRID:109351	HLA-B	BioGRID	PubMed	Reconstituted Complex
BioGRID:107363	BioGRID:109352	HLA-C	BioGRID	PubMed	Reconstituted Complex
BioGRID:107363	BioGRID:109380	HLA-G	BioGRID	PubMed	Reconstituted Complex
BioGRID:107363	BioGRID:124219	LNX1	BioGRID	PubMed	Affinity Capture-Western; Co-localization; Reconstituted Complex
BioGRID:107363	BioGRID:128798	LNX2	BioGRID	PubMed	Affinity Capture-Western; Co-localization; Reconstituted Complex; Two-hybrid
BioGRID:107363	BioGRID:111752	PTPRC	BioGRID	PubMed	FRET
BioGRID:107363	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western

Function	Evidence Code	Pubs
MHC class I protein binding	NAS Non-traceable Author Statement more info	PubMed
coreceptor activity	NAS Non-traceable Author Statement more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein homodimerization activity	IEA Inferred from Electronic Annotation more info
protein kinase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
T cell activation	NAS Non-traceable Author Statement more info	PubMed
T cell mediated immunity	IEA Inferred from Electronic Annotation more info
antigen processing and presentation	NAS Non-traceable Author Statement more info	PubMed
cell surface receptor signaling pathway	IEA Inferred from Electronic Annotation more info
cytotoxic T cell differentiation	IEA Inferred from Electronic Annotation more info
defense response to virus	IEA Inferred from Electronic Annotation more info
immune response	NAS Non-traceable Author Statement more info	PubMed
positive regulation of calcium-mediated signaling	IEA Inferred from Electronic Annotation more info
regulation of immune response	TAS Traceable Author Statement more info
transmembrane receptor protein tyrosine kinase signaling pathway	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
T cell receptor complex	NAS Non-traceable Author Statement more info	PubMed
external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
extracellular region	IEA Inferred from Electronic Annotation more info
integral to plasma membrane	NAS Non-traceable Author Statement more info	PubMed
plasma membrane	TAS Traceable Author Statement more info

General protein information

Preferred Names: T-cell surface glycoprotein CD8 alpha chain

Names: T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_011608.2 RefSeqGene

Range

5001..28792

Download

GenBank, FASTA, Sequence Viewer (Graphics), LRG_44

mRNA and Protein(s)

NM_001145873.1 → NP_001139345.1 T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (3) represents use of an alternate promoter and 5' UTR, compared to variant 1. Both variants 1 and 3 encode the longer, membrane associated isoform (1).

Source sequence(s)

AA668685, AK124156, BC025715, DA075688

Consensus CDS

CCDS1992.1

UniProtKB/Swiss-Prot

P01732

UniProtKB/TrEMBL

Q6ZVS2

UniProtKB/TrEMBL

Q8TAW8

Related

ENSP00000386559, OTTHUMP00000203721, ENST00000409511, OTTHUMT00000330784

Conserved Domains (2) summary

cd05720
Location:31 – 133
Blast Score: 406

Ig_CD8_alpha; Immunoglobulin (Ig) like domain of CD8 alpha chain

pfam07686
Location:21 – 127
Blast Score: 153

V-set; Immunoglobulin V-set domain
NM_001768.6 → NP_001759.3 T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer, membrane associated isoform (1). Both variants 1 and 3 encode the same isoform (1).

Source sequence(s)

AC064848, AK124156, BC025715, DB129686

Consensus CDS

CCDS1992.1

UniProtKB/Swiss-Prot

P01732

UniProtKB/TrEMBL

Q6ZVS2

UniProtKB/TrEMBL

Q8TAW8

Related

ENSP00000283635, OTTHUMP00000160764, ENST00000283635, OTTHUMT00000252596

Conserved Domains (2) summary

cd05720
Location:31 – 133
Blast Score: 406

Ig_CD8_alpha; Immunoglobulin (Ig) like domain of CD8 alpha chain

pfam07686
Location:21 – 127
Blast Score: 153

V-set; Immunoglobulin V-set domain
NM_171827.3 → NP_741969.1 T-cell surface glycoprotein CD8 alpha chain isoform 2 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) lacks an internal segment including the transmembrane domain compared to isoform 1, resulting in a secreted protein.

Source sequence(s)

AC064848, BC025715, DB129686

Consensus CDS

CCDS1993.1

UniProtKB/Swiss-Prot

P01732

UniProtKB/TrEMBL

Q8TAW8

Related

ENSP00000321631, OTTHUMP00000160760, ENST00000352580, OTTHUMT00000252592

Conserved Domains (2) summary

cd05720
Location:31 – 133
Blast Score: 388

Ig_CD8_alpha; Immunoglobulin (Ig) like domain of CD8 alpha chain

pfam07686
Location:21 – 127
Blast Score: 150

V-set; Immunoglobulin V-set domain

RNA

NR_027353.1 RNA Sequence

Description

Transcript Variant: This variant (4) uses an alternate 5' splice pattern, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is predicted to interfere with translation of the longest ORF; translation of the upstream ORF renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC064848, AK300089, BC025715, DB129686

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000002.11 Reference GRCh37.p5 Primary Assembly

Range

87011728..87035519, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000134.1 Alternate HuRef

Range

86898000..86921798, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC064848.5	AAX88864.1
genomic	AC112696.4	None
genomic	CH471053.2	EAW99438.1
		EAW99439.1
		EAW99440.1
		EAW99441.1
genomic	M26313.1	AAA79218.1
genomic	M26314.1	AAA79218.1
genomic	M26315.1	AAA79217.1
		AAA79218.1
genomic	M27161.1	AAA59674.1
mRNA	AA668685.1	None
mRNA	AK097942.1	BAG53555.1
mRNA	AK124156.1	BAC85789.1
mRNA	AK300089.1	BAG61892.1
mRNA	AY039664.1	AAK72403.1
mRNA	BC025715.1	AAH25715.1
mRNA	DA075688.1	None
mRNA	DB129686.1	None
mRNA	M12824.1	AAA61133.1
mRNA	M12828.1	AAB04637.1
other-genetic	DQ893323.2	ABM84249.1
other-genetic	DQ896638.2	ABM87637.1
other-genetic	DQ896639.2	ABM87638.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P01732.1	GenPept	UniProtKB/Swiss-Prot:P01732
Q6ZVS2	GenPept	UniProtKB/TrEMBL:Q6ZVS2
Q8TAW8	GenPept	UniProtKB/TrEMBL:Q8TAW8
Q96QR6	GenPept	UniProtKB/TrEMBL:Q96QR6