CRB3 crumbs homolog 3 (Drosophila) [Homo sapiens (human)] - Gene

Summary

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Official Symbol: CRB3provided by HGNC
Official Full Name: crumbs homolog 3 (Drosophila)provided by HGNC
Primary source: HGNC:20237
Locus tag: UNQ588/PRO1158
See related: Ensembl:ENSG00000130545; HPRD:16749; MIM:609737; Vega:OTTHUMG00000181828
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary: This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Genomic context

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Location :: 19p13.3

Sequence :: Chromosome: 19; NC_000019.9 (6464260..6467232)

See CRB3 in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

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Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Morg1 facilitates Par6-aPKC binding to Crb3 for definition of apical identity of epithelial cells.
Title: The WD40 protein Morg1 facilitates Par6-aPKC binding to Crb3 for apical identity in epithelial cells.
overexpression of human CRB1 and related isoforms, CRB2 and CRB3, had no effect on the levels of presenilin complex components, on NCT maturation or on PS endoproteolysis
Title: Overexpression of human CRB1 or related isoforms, CRB2 and CRB3, does not regulate the human presenilin complex in culture cells.
Data show that a novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta1 interactions.
Title: A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions.
CRB3, through its cytoplasmic domain and its interactors, plays a role in apical membrane morphogenesis and tight junction regulation.
Title: CRB3 binds directly to Par6 and regulates the morphogenesis of the tight junctions in mammalian epithelial cells.
The crumbs3 protein plays an important role in epithelial tight junction formation, and also provide the first known functional role for the mammalian Crumbs FERM binding domain.
Title: Multiple regions of Crumbs3 are required for tight junction formation in MCF10A cells.
the Crumbs3-Pals1 complex has a role in development of polarity in mammalian epithelial cells
Title: The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Interactions

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Products	Interactant	Other Gene	Source	Pubs
Q9BUF7	Q8NI35	INADL	HPRD	PubMed
Q9BUF7	Q8N3R9	MPP5	HPRD	PubMed
Q9BUF7	Q9NPB6	PARD6A	HPRD	PubMed

Pathways from BioSystems

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Cell junction organization, organism-specific biosystem (from REACTOME)
Cell junction organization, organism-specific biosystem
Cell junction organization
Cell-Cell communication, organism-specific biosystem (from REACTOME)
Cell-Cell communication, organism-specific biosystemCell-to-Cell communication is crucial for multicellular organisms because it allows organisms to coordinate the activity of their cells. Some cell-to-cell communication requires direct cell-cell cont...
Cell-cell junction organization, organism-specific biosystem (from REACTOME)
Cell-cell junction organization, organism-specific biosystemEpithelial cell-cell contacts consist of three major adhesion systems: adherens junctions (AJs), tight junctions (TJs), and desmosomes. These adhesion systems differ in their function and compositio...
Tight junction, organism-specific biosystem (from KEGG)
Tight junction, organism-specific biosystemEpithelial tight junctions (TJs) are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of ma...
Tight junction, conserved biosystem (from KEGG)
Tight junction, conserved biosystemEpithelial tight junctions (TJs) are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of ma...
Tight junction interactions, organism-specific biosystem (from REACTOME)
Tight junction interactions, organism-specific biosystemTight junctions (TJs) are the most apical component of the epithelial junctional complex forming a belt-like structure at the cellular junction. When visualized by freeze-fracture electron microscopy...

General gene information

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Homology

Homologs of the CRB3 gene: The CRB3 gene is conserved in Rhesus monkey, mouse, and rat.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
SH3 domain binding	IPI Inferred from Physical Interaction more info	PubMed
protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
cell junction assembly	TAS Traceable Author Statement more info
cell-cell junction organization	TAS Traceable Author Statement more info
protein localization to plasma membrane	IDA Inferred from Direct Assay more info	PubMed
tight junction assembly	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
apical plasma membrane	IEA Inferred from Electronic Annotation more info
integral to membrane	IEA Inferred from Electronic Annotation more info
plasma membrane	TAS Traceable Author Statement more info
tight junction	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein crumbs homolog 3

Names: protein crumbs homolog 3; crumbs protein homolog 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029879.1 RefSeqGene

Range

5001..7973

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_139161.3 → NP_631900.1 protein crumbs homolog 3 isoform a precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) represents the longer transcript but encodes the shorter isoform (a).

Source sequence(s)

AF503290, BC002652, CN403242

Consensus CDS

CCDS12167.1

UniProtKB/Swiss-Prot

Q9BUF7

Related

ENSP00000472010, OTTHUMP00000267595, ENST00000600229, OTTHUMT00000457838
NM_174881.2 → NP_777377.1 protein crumbs homolog 3 isoform b precursor

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks a segment of the coding region, compared to variant 1, causing a frameshift. The resulting isoform (b) contains a longer and distinct C-terminus compared to isoform a.

Source sequence(s)

AY358684, BC002652, CN403242

Consensus CDS

CCDS12166.1

UniProtKB/Swiss-Prot

Q9BUF7

Related

ENSP00000349204, OTTHUMP00000267596, ENST00000356762, OTTHUMT00000457839

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p10 Primary Assembly

Range

6464260..6467232

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GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

6227050..6230022

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GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018930.1 Alternate CHM1_1.0

Range

6389183..6392155

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GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01051456.1 (2709..5681)	None
genomic	AC010503.8 (27466..30910)	None
genomic	AMYH01009373.1 (3005..5977)	None
genomic	CH471139.2	EAW69083.1
		EAW69084.1
		EAW69085.1
		EAW69086.1
mRNA	AF503290.1	AAM23013.1
mRNA	AK000653.1	None
mRNA	AK292956.1	BAF85645.1
mRNA	AY103469.1	AAM44074.1
mRNA	AY358684.1	AAQ89047.1
mRNA	BC002652.2	AAH02652.2
mRNA	BC018409.1	AAH18409.1
mRNA	BM129809.1	None
mRNA	CN403242.1	None