DCLK1 doublecortin-like kinase 1 [Homo sapiens] - Gene

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Summary

Official Symbol: DCLK1provided by HGNC
Official Full Name: doublecortin-like kinase 1provided by HGNC
Primary source: HGNC:2700
Locus tag: RP11-113P14.1
See related: Ensembl:ENSG00000133083; HPRD:09202; MIM:604742; Vega:OTTHUMG00000016729
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1; KIAA0369
Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]

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Genomic context

Location :: 13q13

Sequence :: Chromosome: 13; NC_000013.10 (36342789..36705514, complement)

See DCLK1 in Epigenomics, MapViewer

Chromosome 13 - NC_000013.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High DCAMKL1 is associated with HCV-induced hepatocarcinogenesis.
Title: Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.
Eight suggestive significant loci were detected with a series of genes expressed within the inner ear that underlie the auditory function, such as: DCLK1, PTPRD, GRM8, CMIP.
Title: Hearing function and thresholds: a genome-wide association study in European isolated populations identifies new loci and pathways.
Data show that siRNA-mediated knockdown of DCAMKL-1 in pancreatic cancer cells induced microRNA miR-200a, an EMT inhibitor, along with downregulation of EMT-associated transcription factors.
Title: DCAMKL-1 regulates epithelial-mesenchymal transition in human pancreatic cells through a miR-200a-dependent mechanism.
Together, our results suggest that silencing the DCLK splice variants DCL and DCLK-long induces apoptosis in NB cells.
Title: Silencing of the microtubule-associated proteins doublecortin-like and doublecortin-like kinase-long induces apoptosis in neuroblastoma cells.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Observational study of gene-disease association. (HuGE Navigator)
Title: Variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, are associated with memory and general cognitive abilities.
an examination of the binding sites on this protein
Title: The DCX-domain tandems of doublecortin and doublecortin-like kinase.
genomic organization underlying the splice variants of DCLK and the expression profile of two splice variants affecting the kinase domain of DCLK and CPG16 (candidate plasticity gene 16).
Title: Alternative splice variants of doublecortin-like kinase are differentially expressed and have different kinase activities.
high-quality wild-type crystals of doublecortin-like kinase were obtained and a complete native data set was collected to 1.5 A resolution
Title: Purification and crystallization of the N-terminal domain from the human doublecortin-like kinase.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

A genome-wide association study of optic disc parameters.

Genome-wide association study of electrocardiographic and heart rate variability traits: the Framingham Heart Study.

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Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
kinase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein kinase activity	TAS Traceable Author Statement more info	PubMed
protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
receptor signaling protein activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
cell differentiation	IEA Inferred from Electronic Annotation more info
central nervous system development	TAS Traceable Author Statement more info	PubMed
endosome transport	NAS Non-traceable Author Statement more info	PubMed
intracellular signal transduction	IEA Inferred from Electronic Annotation more info
multicellular organismal development	IEA Inferred from Electronic Annotation more info
nervous system development	TAS Traceable Author Statement more info	PubMed
protein phosphorylation	TAS Traceable Author Statement more info	PubMed
response to virus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
integral to plasma membrane	TAS Traceable Author Statement more info	PubMed

General protein information

Preferred Names: serine/threonine-protein kinase DCLK1

Names: serine/threonine-protein kinase DCLK1; doublecortin and CaM kinase-like 1; doublecortin-like and CAM kinase-like 1; doublecortin domain-containing protein 3A

NP_001182344.1

EC 2.7.11.1

NP_001182345.1

EC 2.7.11.1

NP_001182359.1

EC 2.7.11.1

NP_004725.1

EC 2.7.11.1

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001195415.1 → NP_001182344.1 serine/threonine-protein kinase DCLK1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as DCLK-short-B, DCK-beta1 or KIAA0369-BS) is produced from the 3' promoter. It lacks several 5' exons but has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and different N-terminus, and lacks doublecortin domains, as compared to isoform 1.

Source sequence(s)

AK295777, AK311195, AL139083, AL157760

UniProtKB/Swiss-Prot

O15075

Related

ENSP00000382257, ENST00000399319

Conserved Domains (2) summary

smart00220
Location:83 – 340
Blast Score: 823

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:83 – 339
Blast Score: 565

PKc_like; Protein Kinases, catalytic domain
NM_001195416.1 → NP_001182345.1 serine/threonine-protein kinase DCLK1 isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant [3, also known as DCLK-short-A, DCK-beta2, KIAA0369-BL or cpg16 (candidate plasticity gene 16)] is produced from the 3' promoter. It lacks several 5' exons but has an alternate 5' exon, and lacks a coding exon in the 3' region that results in a frame-shift, as compared to variant 1. The resulting isoform (3) has a shorter and different N-terminus and a longer and different C-terminus, and lacks doublecortin domains, as compared to isoform 1.

Source sequence(s)

AK299059, AK311195, AL139083, AL157760, BC152456

Consensus CDS

CCDS55895.1

UniProtKB/TrEMBL

B7Z5K4

UniProtKB/Swiss-Prot

O15075

Related

ENSP00000369223, OTTHUMP00000018252, ENST00000379893, OTTHUMT00000044488

Conserved Domains (2) summary

smart00220
Location:83 – 340
Blast Score: 832

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:83 – 339
Blast Score: 570

PKc_like; Protein Kinases, catalytic domain
NM_001195430.1 → NP_001182359.1 serine/threonine-protein kinase DCLK1 isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) is produced from the 3' promoter. It shares only one exon with variant 1, and encodes a 56 aa long isoform [4, also known as ania-4, or CARP (CaMK-related peptide)], which mainly consists of the serine/proline-rich domain.

Source sequence(s)

AK311195, AL157760, BM682165, CR627093
NM_004734.4 → NP_004725.1 serine/threonine-protein kinase DCLK1 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as DCLK-long-B, DCK-alpha1 or KIAA0369AS) is produced from the 5' promoter. It encodes the longest isoform (1), which includes two doublecortin domains, a serine/proline-rich domain and a protein kinase domain.

Source sequence(s)

AK295777, AL139083, AL157760, AL160392, BC152456

Consensus CDS

CCDS9354.1

UniProtKB/Swiss-Prot

O15075

Related

ENSP00000255448, OTTHUMP00000018250, ENST00000255448, OTTHUMT00000044483

Conserved Domains (3) summary

cd01617
Location:57 – 139
Blast Score: 292

DCX; Ubiquitin-like domain of DCX

smart00220
Location:390 – 647
Blast Score: 829

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:390 – 646
Blast Score: 564

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000013.10 Reference GRCh37.p5 Primary Assembly

Range

36342789..36705514, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000145.1 Alternate HuRef

Range

17156100..17334637, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AL139083.15 (159892..162080)	None
genomic	AL157694.7	CAH70656.1
		CAH70657.1
		CAH70658.1
genomic	AL157760.9	CAH70167.1
		CAH70168.1
		CAH70169.1
		CAH70170.1
genomic	AL157760.9	CAH70167.1
		CAH70168.1
		CAH70169.1
		CAH70170.1
genomic	AL160392.12	CAH70261.1
		CAH70262.1
		CAH70263.1
genomic	AL390029.35	CAI15720.1
		CAI15721.1
		CAI15722.1
genomic	CH471075.1	EAX08550.1
		EAX08551.1
genomic	HI584573.1	CBX55086.1
mRNA	AB002367.1	BAA20824.2
mRNA	AF052152.1	None
mRNA	AF070534.1	None
mRNA	AK295102.1	BAH11973.1
mRNA	AK295777.1	BAH12185.1
mRNA	AK299059.1	BAH12940.1
mRNA	AK299829.1	BAH13141.1
mRNA	AK308925.1	None
mRNA	AK311183.1	None
mRNA	AK311195.1	None
mRNA	AK311434.1	None
mRNA	AK311438.1	None
mRNA	AK311459.1	None
mRNA	BC152456.1	AAI52457.1
mRNA	BE672499.1	None
mRNA	BI824620.1	None
mRNA	BM682165.1	None
mRNA	BQ711413.1	None
mRNA	CD619255.1	None
mRNA	CR627093.1	None
other-genetic	BC160043.1	AAI60043.1