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    PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens ]

    Gene ID: 9088, updated on 11-May-2012
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    Summary

    Official Symbol
    PKMYT1provided by HGNC
    Official Full Name
    protein kinase, membrane associated tyrosine/threonine 1provided by HGNC
    Primary source
    HGNC:29650
    See related
    Ensembl:ENSG00000127564; HPRD:03920; MIM:602474; Vega:OTTHUMG00000128975
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MYT1; FLJ20093; DKFZp547K1610
    Summary
    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
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    Genomic context

    Location :
    16p13.3
    Sequence :
    Chromosome: 16; NC_000016.9 (3022792..3030540, complement)
    See PKMYT1 in Epigenomics, MapViewer

    Chromosome 16 - NC_000016.9Genomic Context describing neighboring genes Neighboring gene FLYWCH-type zinc finger 1 Neighboring gene 60S ribosomal protein L23a-like Neighboring gene kringle containing transmembrane protein 2 Neighboring gene progestin and adipoQ receptor family member IV Neighboring gene long intergenic non-protein coding RNA 514 Neighboring gene uncharacterized LOC100507378

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. In vitro and in silico studies on substrate recognition and acceptance of human PKMYT1, a Cdk1 inhibitory kinase. Rohe A, et al. Bioorg Med Chem Lett, 2012 Jan 15. PMID 22189141.
    2. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
    3. A genome-wide association study of the metabolic syndrome in Indian Asian men. Zabaneh D, et al. PLoS One, 2010 Aug 4. PMID 20694148.
    4. Systematic analysis of human protein complexes identifies chromosome segregation proteins. Hutchins JR, et al. Science, 2010 Apr 30. PMID 20360068.
    5. Docking motif-guided mapping of the interactome of protein phosphatase-1. Hendrickx A, et al. Chem Biol, 2009 Apr 24. PMID 19389623.

    See all (24) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. The results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis in Hela cells.
      Title: Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit.
    2. MYT1 binds to TSAP6 in tumor cells and has a role in cell cycle regulation
      Title: The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
    3. the induction of Cdc2 phosphorylation due to the increase of Wee1 and Myt1 as well as the reduction of Cdc2 and cyclin B1 are involved in 1,25[OH]2VD3-induced G2/M arrest of keratinocytes.
      Title: Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
    4. overexpressed during the S phase of the cell cycle compared with the G0/1 phase
      Title: Identifying cellular genes crucial for the reactivation of Kaposi's sarcoma-associated herpesvirus latency.
    5. Myt1 is phosphorylated by polo-like kinase 1
      Title: Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

    A genome-wide association study of the metabolic syndrome in Indian Asian men.

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_004194.3 NP_001777.1 CDK1    BIND  PubMed Myt1 interacts with and phosphorylates Cdc2. This interaction was modelled on a demonstrated interaction between human Myt1 and Cdc2 from an unspecified species. 
    NP_004194.3 NP_006212.1 PIN1    BIND  PubMed Pin1 interacts with phosphorylated Myt1. 
    Q99640 P10415 BCL2    HPRD  PubMed  
    Q99640 P14635 CCNB1    HPRD  PubMed  
    Q99640 P06493 CDK1    HPRD  PubMed  
    Q99640 Q13526 PIN1    HPRD  PubMed  
    Q99640 P53350 PLK1    HPRD  PubMed  
    Q99640 Dudulin 2 STEAP3    HPRD  PubMed  
    BioGRID:114544 BioGRID:198624 Cdk1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:114544 BioGRID:111317 PIN1    BioGRID  PubMed Affinity Capture-Western; Far Western 
    BioGRID:114544 BioGRID:120533 STEAP3    BioGRID  PubMed Affinity Capture-Western; Two-hybrid 
    BioGRID:114544 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
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    General gene information

    Markers

    Genotypes

    Homology

    Pathways from BioSystems

    • Cell Cycle, organism-specific biosystem (from REACTOME)
      Cell Cycle, organism-specific biosystem
      Cell Cycle
    • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
      Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
    • Cell cycle, organism-specific biosystem (from WikiPathways)
      Cell cycle, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
    • Cell cycle, organism-specific biosystem (from KEGG)
      Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
    • Cell cycle, conserved biosystem (from KEGG)
      Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
    • Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem (from REACTOME)
      Cyclin A/B1 associated events during G2/M transition, organism-specific biosystemCell cycle progression is regulated by cyclin-dependent protein kinases at both the G1/S and the G2/M transitions by cyclin-dependent protein kinases. The G2/M transition is regulated through the pho...
    • Cyclin E associated events during G1/S transition, organism-specific biosystem (from REACTOME)
      Cyclin E associated events during G1/S transition, organism-specific biosystemThe transition from the G1 to S phase is controlled by the Cyclin E:Cdk2 complexes. As the Cyclin E:Cdk2 complexes are formed, the Cdk2 is phosphorylated by the Wee1 and Myt1 kinases. This phosphoryl...
    • G1/S Transition, organism-specific biosystem (from REACTOME)
      G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
    • G2/M Transition, organism-specific biosystem (from REACTOME)
      G2/M Transition, organism-specific biosystemCyclin A can also form complexes with Cdc2 (Cdk1). Together with three B-type cyclins, Cdc2 (Cdk1) regulates the transition from G2 into mitosis. These complexes are activated by dephosphorylation of...
    • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
      Mitotic G1-G1/S phases, organism-specific biosystem
      Mitotic G1-G1/S phases
    • Mitotic G2-G2/M phases, organism-specific biosystem (from REACTOME)
      Mitotic G2-G2/M phases, organism-specific biosystem
      Mitotic G2-G2/M phases
    • Oocyte meiosis, organism-specific biosystem (from KEGG)
      Oocyte meiosis, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, homologous chromosomes recombine and then segrega...
    • Oocyte meiosis, conserved biosystem (from KEGG)
      Oocyte meiosis, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, homologous chromosomes recombine and then segrega...
    • Polo-like kinase mediated events, organism-specific biosystem (from REACTOME)
      Polo-like kinase mediated events, organism-specific biosystemAt mitotic entry, Plk1 phosphorylates and activates Cdc25C phosphatase, whereas it phosphorylates and down-regulates Wee1A. Plk1 also phosphorylates and inhibits Myt1 activity. Cyclin B1-bound Cdc2...
    • Progesterone-mediated oocyte maturation, organism-specific biosystem (from KEGG)
      Progesterone-mediated oocyte maturation, organism-specific biosystemXenopus oocytes are naturally arrested at G2 of meiosis I. Exposure to either insulin/IGF-1 or the steroid hormone progesterone breaks this arrest and induces resumption of the two meiotic division c...
    • Progesterone-mediated oocyte maturation, conserved biosystem (from KEGG)
      Progesterone-mediated oocyte maturation, conserved biosystemXenopus oocytes are naturally arrested at G2 of meiosis I. Exposure to either insulin/IGF-1 or the steroid hormone progesterone breaks this arrest and induces resumption of the two meiotic division c...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    protein kinase activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    G1/S transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    G2/M transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    mitosis TAS
    Traceable Author Statement
    more info
    		 PubMed 
    mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    negative regulation of phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    protein phosphorylation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    regulation of cell cycle TAS
    Traceable Author Statement
    more info
    		  
    regulation of cyclin-dependent protein kinase activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    regulation of mitosis TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    membrane fraction TAS
    Traceable Author Statement
    more info
    		 PubMed 
    nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Preferred Names
    membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
    Names
    membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
    myt1 kinase
    NP_004194.3
    NP_872629.1
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001258450.1 → NP_001245379.1  membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
      Source sequence(s)
      AK301926, BQ017689, DB111506

    2. NM_001258451.1 → NP_001245380.1  membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 4

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1.
      Source sequence(s)
      AK097642, AK301926, BQ017689, DB111506

    3. NM_004203.4 → NP_004194.3  membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      BC121161, BQ017689, DB009060, DB111506
      Consensus CDS
      CCDS10486.1
      UniProtKB/TrEMBL
      Q0IJ49
      UniProtKB/Swiss-Prot
      Q99640
      Related
      ENSP00000262300, OTTHUMP00000159225, ENST00000262300, OTTHUMT00000250963
      Conserved Domains (2) summary
      smart00220
      Location:110 – 357
      Blast Score: 514
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cl09925
      Location:110 – 359
      Blast Score: 475
      PKc_like; Protein Kinases, catalytic domain

    4. NM_182687.2 → NP_872629.1  membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AK098452, BQ017689, DB009060, DB111506
      Consensus CDS
      CCDS45391.1
      UniProtKB/TrEMBL
      F8W164
      Related
      ENSP00000397739, ENST00000440027
      Conserved Domains (2) summary
      smart00220
      Location:110 – 357
      Blast Score: 510
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cl09925
      Location:110 – 359
      Blast Score: 474
      PKc_like; Protein Kinases, catalytic domain

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000016.9 Reference GRCh37.p5 Primary Assembly

      Range
      3022792..3030540, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000148.1 Alternate HuRef

      Range
      2995283..3002445, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC004233.1 AAC04478.1
    genomic AC004235.1 AAC04477.1
    genomic AF549406.1 AAN40703.1
    genomic CH471112.2 EAW85439.1
      EAW85440.1
    mRNA AK097642.1 BAG53500.1
    mRNA AK098452.1 None
    mRNA AK294379.1 BAG57637.1
    mRNA AK301926.1 BAG63346.1
    mRNA AK303005.1 BAG64138.1
    mRNA AK309964.1 None
    mRNA BC121161.1 AAI21162.1
    mRNA BC121162.1 AAI21163.1
    mRNA BC130011.1 AAI30012.1
    mRNA BC153823.1 None
    mRNA BG530406.1 None
    mRNA BQ017689.1 None
    mRNA DB009060.1 None
    mRNA DB111506.1 None
    mRNA U56816.1 AAC50949.1
    other-genetic BC160025.1 AAI60025.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q0IJ49 GenPept UniProtKB/TrEMBL:Q0IJ49
    Q99640.1 GenPept UniProtKB/Swiss-Prot:Q99640
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Medical
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous

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