CCNT2 cyclin T2 [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: CCNT2provided by HGNC
Official Full Name: cyclin T2provided by HGNC
Primary source: HGNC:1600
See related: Ensembl:ENSG00000082258; HPRD:04839; MIM:603862; Vega:OTTHUMG00000131712
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CYCT2; FLJ13583; FLJ90560; MGC134840
Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. A pseudogene of this gene is found on chromosome 1. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]

Help top of page

Genomic context

Location :: 2q21.3

Sequence :: Chromosome: 2; NC_000002.11 (135676392..135716915)

See CCNT2 in Epigenomics, MapViewer

Chromosome 2 - NC_000002.11 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
The tripartite protein-RNA complex formation between Hexim, Cyclin T and 7SK snRNA, was analyzed.
Title: Specificity of Hexim1 and Hexim2 complex formation with cyclin T1/T2, importin alpha and 7SK snRNA.
TAF7 interacts with the transcription factors, TFIIH and P-TEFb, resulting in the inhibition of their Pol II CTD kinase activities
Title: TFIID component TAF7 functionally interacts with both TFIIH and P-TEFb.
These results suggest that acetylation of CDK9 is an important posttranslational modification that is involved in regulating P-TEFb transcriptional elongation function.
Title: Regulation of P-TEFb elongation complex activity by CDK9 acetylation.
Data strengthen the hypothesis that Cyclin T2a plays a role in muscle differentiation, and propose PKNalpha as a novel partner of Cyclin T2a in this process.
Title: Pkn is a novel partner of cyclin T2a in muscle differentiation.
interaction with pRb
Title: Physical interaction between pRb and cdk9/cyclinT2 complex.
CycT2 not only contains two domains that target rna polymerase II but this substrate recognition is necessary for its transcriptional activity via DNA
Title: Transcriptional activity and substrate recognition of cyclin T2 from P-TEFb.
The results establish that cdk9/cyclin T2a-mediated coactivation of MyoD depends on serine 37 phosphorylation.
Title: Abrogation of signal-dependent activation of the cdk9/cyclin T2a complex in human RD rhabdomyosarcoma cells.

Submit: New GeneRIF Correction

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Help top of page

HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Cyclin T2 competes with cyclin T1 for binding to CDK9 and can thereby inhibit HIV-1 Tat activity	PubMed
	tat	Amino acids 260-263 of cyclin T1 are critical for HIV-1 Tat-mediated transcriptional activation, and site-directed mutations in this region of cyclin T2 (asparagine to cysteine at residue 260) allow it to bind Tat and stimulate transcription	PubMed

Go to the HIV-1, Human Protein Interaction Database

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
NP_001232.1	NP_001252.1	CDK9	BIND	PubMed	CyclinT2a interacts with Cdk9.
NP_490595.1	NP_001252.1	CDK9	BIND	PubMed	CyclinT2b interacts with Cdk9.
O60583	P50750	CDK9	HPRD	PubMed
O60583	Cardiac lineage protein 1	HEXIM1	HPRD	PubMed
O60583	Q16512	PKN1	HPRD	PubMed
O60583	P06400	RB1	HPRD	PubMed
BioGRID:107344	BioGRID:117036	BRD4	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107344	BioGRID:107459	CDK9	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Reconstituted Complex
BioGRID:107344	BioGRID:115860	HEXIM1	BioGRID	PubMed	Two-hybrid
BioGRID:107344	BioGRID:121122	MEPCE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107344	BioGRID:110446	MLLT3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107344	BioGRID:111860	RB1	BioGRID	PubMed	Reconstituted Complex
BioGRID:107344	BioGRID:112873	TERF2	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
protein kinase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cell cycle	IEA Inferred from Electronic Annotation more info
cell division	IEA Inferred from Electronic Annotation more info
gene expression	TAS Traceable Author Statement more info
interspecies interaction between organisms	IEA Inferred from Electronic Annotation more info
regulation of cyclin-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
transcription elongation from RNA polymerase II promoter	TAS Traceable Author Statement more info
transcription from RNA polymerase II promoter	TAS Traceable Author Statement more info
viral reproduction	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
nucleoplasm	TAS Traceable Author Statement more info
nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: cyclin-T2

Names: cyclin-T2; cyclin T2a; cyclin T2b; SDS-stable vimentin-bound DNA fragment HEF42VIM22; subunit of positive elongation transcription factor b

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029189.1 RefSeqGene

Range

5001..45524

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001241.3 → NP_001232.1 cyclin-T2 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (a) encodes isoform a.

Source sequence(s)

AC016725, AK294889, AU157699, BC114366

Consensus CDS

CCDS2175.1

UniProtKB/TrEMBL

B4DH21

UniProtKB/Swiss-Prot

O60583

Related

ENSP00000295238, OTTHUMP00000162503, ENST00000295238, OTTHUMT00000254630

Conserved Domains (3) summary

cd00043
Location:37 – 108
Blast Score: 158

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...

COG5333
Location:8 – 216
Blast Score: 217

CCL1; Cdk activating kinase (CAK)/RNA polymerase II transcription initiation/nucleotide excision repair factor TFIIH/TFIIK, cyclin H subunit [Cell division and chromosome partitioning / Transcription / DNA replication, recombination, and repair]

COG5422
Location:296 – 427
Blast Score: 90

ROM1; RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms]
NM_058241.2 → NP_490595.1 cyclin-T2 isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (b) contains an additional internal fragment in the 3' coding region, which results in a frameshift, compared to variant a. The resulting isoform (b) is longer and has a distinct C-terminus, compared to isoform a.

Source sequence(s)

AC016725, AK292913, AK294889

Consensus CDS

CCDS2174.1

UniProtKB/TrEMBL

B4DH21

UniProtKB/Swiss-Prot

O60583

Related

ENSP00000264157, OTTHUMP00000162502, ENST00000264157, OTTHUMT00000254629

Conserved Domains (3) summary

cd00043
Location:37 – 108
Blast Score: 158

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...

COG5333
Location:8 – 216
Blast Score: 215

CCL1; Cdk activating kinase (CAK)/RNA polymerase II transcription initiation/nucleotide excision repair factor TFIIH/TFIIK, cyclin H subunit [Cell division and chromosome partitioning / Transcription / DNA replication, recombination, and repair]

COG5422
Location:296 – 427
Blast Score: 90

ROM1; RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms]

RNA

NR_037649.1 RNA Sequence

Description

Transcript Variant: This variant (c) has an addition exon, compared to variant a. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant a, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC016725, AK294889

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000002.11 Reference GRCh37.p5 Primary Assembly

Range

135676392..135716915

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000134.1 Alternate HuRef

Range

127668086..127708608

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC016725.5	AAY14998.1
genomic	AJ297062.1	None
genomic	AY865621.1	AAW56073.1
genomic	CH471058.2	EAX11644.1
		EAX11645.1
		EAX11646.1
		EAX11647.1
mRNA	AF048731.1	AAC39665.1
mRNA	AF048732.1	AAC39666.1
mRNA	AK023645.1	None
mRNA	AK075041.1	None
mRNA	AK292913.1	BAF85602.1
mRNA	AK294889.1	BAG57982.1
mRNA	AU157699.1	None
mRNA	BC114366.2	AAI14367.1
mRNA	BX648174.1	None