UBE2M ubiquitin-conjugating enzyme E2M [Homo sapiens] - Gene

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Summary

Official Symbol: UBE2Mprovided by HGNC
Official Full Name: ubiquitin-conjugating enzyme E2Mprovided by HGNC
Primary source: HGNC:12491
See related: Ensembl:ENSG00000130725; HPRD:04414; MIM:603173
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: UBC12; hUbc12; UBC-RS2
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 19q13.43

Sequence :: Chromosome: 19; NC_000019.9 (59067079..59070343, complement)

See UBE2M in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results demonstrate a role for UBE2M in mediating cytotoxicity of gemcitabine in human urothelial carcinoma cells.
Title: UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.
SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation
Title: SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.
crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12)
Title: Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
P61081	Q99829	CPNE1	HPRD	PubMed
P61081	Q96FN4	CPNE2	HPRD	PubMed
P61081	Q96A23	CPNE4	HPRD	PubMed
P61081	Q15843	NEDD8	HPRD	PubMed
P61081	Q8TBC4	UBA3	HPRD	PubMed
BioGRID:114504	BioGRID:107315	CBL	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114504	BioGRID:107452	CDK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:115159	CUL7	BioGRID	PubMed	Biochemical Activity
BioGRID:114504	BioGRID:119920	DCUN1D1	BioGRID	PubMed	Reconstituted Complex
BioGRID:114504	BioGRID:116759	DCUN1D4	BioGRID	PubMed	Co-crystal Structure
BioGRID:114504	BioGRID:122284	FAM113A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:121122	MEPCE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:114402	NAE1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:110811	NEDD4	BioGRID	PubMed	Reconstituted Complex
BioGRID:114504	BioGRID:110815	NEDD8	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:114504	BioGRID:115885	NPRL2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:247522	Nedd4	BioGRID	PubMed	Reconstituted Complex
BioGRID:114504	BioGRID:111559	PRKAR1A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:120146	RNF111	BioGRID	PubMed	Two-hybrid
BioGRID:114504	BioGRID:114503	UBA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114504	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
NEDD8 ligase activity	IDA Inferred from Direct Assay more info	PubMed
acid-amino acid ligase activity	IEA Inferred from Electronic Annotation more info
ligase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
ribosomal S6-glutamic acid ligase activity	IDA Inferred from Direct Assay more info	PubMed
ubiquitin-protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
ubiquitin-protein ligase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
positive regulation of neuron apoptosis	IEA Inferred from Electronic Annotation more info
protein modification process	IMP Inferred from Mutant Phenotype more info	PubMed
protein modification process	TAS Traceable Author Statement more info	PubMed
protein neddylation	IDA Inferred from Direct Assay more info	PubMed
protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
protein ubiquitination	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cellular_component	ND No biological Data available more info

General protein information

Preferred Names: NEDD8-conjugating enzyme Ubc12

Names: NEDD8-conjugating enzyme Ubc12; yeast UBC12 homolog; NEDD8 protein ligase; NEDD8 carrier protein; ubiquitin-protein ligase M; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)

NP_003960.1

EC 6.3.2.19

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003969.3 → NP_003960.1 NEDD8-conjugating enzyme Ubc12

Status: REVIEWED

Source sequence(s)

AC016630, BC058924, BM798750

Consensus CDS

CCDS12987.1

UniProtKB/Swiss-Prot

P61081

Related

ENSP00000253023, ENST00000253023

Conserved Domains (1) summary

cd00195
Location:33 – 166
Blast Score: 305

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p5 Primary Assembly

Range

59067079..59070343, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

55377382..55380551, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC016630.6 (6020..9276)	None
genomic	CH471135.1	EAW72612.1
		EAW72613.1
mRNA	AB012191.1	BAA33145.1
mRNA	AF075599.1	AAC26141.1
mRNA	AL569047.3	None
mRNA	BC058924.1	AAH58924.1
mRNA	BM559596.1	None
mRNA	BM798750.1	None
mRNA	BT006754.1	AAP35400.1
mRNA	BU508830.1	None
mRNA	BX342697.2	None