TOP3B topoisomerase (DNA) III beta [Homo sapiens (human)] - Gene

Summary

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Official Symbol: TOP3Bprovided by HGNC
Official Full Name: topoisomerase (DNA) III betaprovided by HGNC
Primary source: HGNC:11993
See related: Ensembl:ENSG00000100038; HPRD:04662; MIM:603582; Vega:OTTHUMG00000167438
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Genomic context

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Location :: 22q11.22

Sequence :: Chromosome: 22; NC_000022.10 (22311403..22337147, complement)

See TOP3B in Epigenomics, MapViewer

Chromosome 22 - NC_000022.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Topoisomerase IIIbeta expression can be a useful marker in assessing the prognosis of patients with breast cancer and is an independent predictor of survival.
Title: Significance of topoisomerase IIIβ expression in breast ductal carcinomas: strong associations with disease-specific survival and metastasis.
the positive regulatory region including the 5'GGAACC3' core element is essential for efficient expression of the DNA topoisomerase III beta gene
Title: Identification of the functional elements in the promoter region of human DNA topoisomerase IIIbeta gene.
Two isoforms of human DNA topoisomerase IIIbeta were expressed in HeLa cells. Isoform 1 was localized to the nucleus and associated with chromosomes during metaphase and anaphase. Isoform 2 was found in the cytoplasm.
Title: M phase-specific association of human topoisomerase IIIbeta with chromosomes.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_003926.1	BAA95953.1	RECQL5	BIND	PubMed	RecQ5-beta interacts with Top3-beta.
O95985	Q07021	C1QBP	HPRD	PubMed
O95985	P49454	CENPF	HPRD	PubMed
O95985	Q9HCK8	CHD8	HPRD	PubMed
O95985	Q9UKJ5	CHIC2	HPRD	PubMed
O95985	Q9UBX5	FBLN5	HPRD	PubMed
O95985	P28799	GRN	HPRD	PubMed
O95985	Q9BQ66	KRTAP4-12	HPRD	PubMed
O95985	O94762	RECQL5	HPRD	PubMed
O95985	P14678	SNRPB	HPRD	PubMed
O95985	Q8IX07	ZFPM1	HPRD	PubMed
O95985	Q9H9D4	ZNF408	HPRD	PubMed
BioGRID:114452	BioGRID:107492	CENPF	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:121709	CHD8	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:117716	CHIC2	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:115771	FBLN5	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:109153	GRN	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:123756	KRTAP4-12	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:122103	MMS19	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114452	BioGRID:114797	RECQL5	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114452	BioGRID:112512	SNRPB	BioGRID	PubMed	Two-hybrid
BioGRID:114452	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114452	BioGRID:127806	ZFPM1	BioGRID	PubMed	Two-hybrid

Pathways from BioSystems

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Bloom's syndrome complex, organism-specific biosystem (from KEGG)
Bloom's syndrome complex, organism-specific biosystemStructural complex; Genetic information processing; Repair system
Fanconi anemia pathway, organism-specific biosystem (from KEGG)
Fanconi anemia pathway, organism-specific biosystemThe Fanconi anemia pathway is required for the efficient repair of damaged DNA, especially interstrand cross-links (ICLs). DNA ICL is directly recognized by FANCM and associated proteins, that recrui...
Fanconi anemia pathway, conserved biosystem (from KEGG)
Fanconi anemia pathway, conserved biosystemThe Fanconi anemia pathway is required for the efficient repair of damaged DNA, especially interstrand cross-links (ICLs). DNA ICL is directly recognized by FANCM and associated proteins, that recrui...
Homologous recombination, organism-specific biosystem (from KEGG)
Homologous recombination, organism-specific biosystemHomologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves...
Homologous recombination, conserved biosystem (from KEGG)
Homologous recombination, conserved biosystemHomologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves...

General gene information

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Markers

Top3b (e-PCR), detects polymorphism
- UniSTS:259698

Homology

Homologs of the TOP3B gene: The TOP3B gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, C.elegans, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
DNA binding	TAS Traceable Author Statement more info	PubMed
DNA topoisomerase activity	TAS Traceable Author Statement more info	PubMed
DNA topoisomerase type I activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
DNA topological change	IEA Inferred from Electronic Annotation more info
chromosome segregation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
condensed chromosome	IEA Inferred from Electronic Annotation more info
nucleus	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase 3-beta-1

Names: DNA topoisomerase 3-beta-1; topoisomerase III beta; DNA topoisomerase III beta-1

NP_003926.1

EC 5.99.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003935.3 → NP_003926.1 DNA topoisomerase 3-beta-1

Status: REVIEWED

Source sequence(s)

BC002432

Consensus CDS

CCDS13797.1

UniProtKB/Swiss-Prot

O95985

Related

ENSP00000381773, OTTHUMP00000198581, ENST00000398793, OTTHUMT00000320251

Conserved Domains (3) summary

cd00186
Location:172 – 593
Blast Score: 1032

TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...

cd03362
Location:3 – 167
Blast Score: 461

TOPRIM_TopoIA_TopoIII; TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving ...

PRK07219
Location:3 – 752
Blast Score: 1130

PRK07219; DNA topoisomerase I; Validated

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000022.10 Reference GRCh37.p10 Primary Assembly

Range

22311403..22337147, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000154.1 Alternate HuRef

Range

5268479..5294196, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018933.1 Alternate CHM1_1.0

Range

6257735..6283454, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01055075.1 (43294..69011)	None
genomic	AMYH01012639.1 (143833..169552)	None
genomic	CH471095.1	EAW59487.1
		EAW59488.1
		EAW59489.1
		EAW59490.1
		EAW59491.1
genomic	D87012.1	BAA20009.1
mRNA	AF017146.1	AAD01614.1
mRNA	AF053082.1	AAD15791.1
mRNA	AF070585.1	None
mRNA	AF125216.1	AAD29670.1
mRNA	AK096695.1	BAG53351.1
mRNA	AK290997.1	BAF83686.1
mRNA	AK304709.1	BAG65474.1
mRNA	AL833505.1	None
mRNA	BC002432.2	AAH02432.1
mRNA	CR456596.1	CAG30482.1
other-genetic	CU013196.1	CAK54627.1
other-genetic	CU013484.1	CAK54926.1