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TKL1 transketolase TKL1 [ Saccharomyces cerevisiae S288c ]

Gene ID: 856188, updated on 11-May-2013

Summary

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Gene symbol: TKL1
Gene description: transketolase TKL1
Primary source: SGD:S000006278
Locus tag: YPR074C
Gene type: protein coding
RNA name: transketolase TKL1
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

Genomic context

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Location :: chromosome: XVI

Sequence :: Chromosome: XVI; NC_001148.4 (692796..694838, complement)

See TKL1 in MapViewer

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

characterization of NQM1 and TKL2, together with TAL1 and TKL1, regarding their roles in xylose utilization and fermentation
Title: Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae.
Halogenated pyruvate derivatives can function as donor substrates of transketolase.
Title: Halogenated pyruvate derivatives as substrates of transketolase from Saccharomyces cerevisiae.
In the presence of Ca2+, the active sites of TK1 and TK2 exhibit negative cooperativity in binding both xylulose-5-phosphate and ribose-5-phosphate and positive cooperativity in the catalytic transformation of the substrates.
Title: Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae.
Review summarizes the results of studies on the kinetics of the interaction of thiamine diphosphate with transketolase and the generation of the catalytically active form of the coenzyme within the holoenzyme and formation of the enzyme's active center.
Title: Binding of the coenzyme and formation of the transketolase active center.
transketolase is regulated by donor substrates
Title: Donor substrate regulation of transketolase.

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Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:36247	BioGRID:31422	ACS2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:31107	ACT1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32503	ADA2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32690	ALK2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32183	ARO1	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:32771	BAP2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31825	CDC13	BioGRID	PubMed	Positive Genetic; Synthetic Rescue
BioGRID:36247	BioGRID:31579	CDC3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33419	CLC1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31654	COX8	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35835	CSE2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31754	CYS3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35014	DAL81	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31148	DEG1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31903	DHH1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:36247	BioGRID:32780	ECM33	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36556	EPT1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31331	ERG3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35161	ERG6	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33773	ESS1	BioGRID	PubMed	Protein-peptide
BioGRID:36247	BioGRID:31157	FAR7	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36801	FCY21	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31017	FEN1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33847	FIP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:31830	GDH2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32559	GIN4	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31130	GNA1	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:36616	GND1	BioGRID	PubMed	Negative Genetic; Synthetic Lethality
BioGRID:36247	BioGRID:33508	GND2	BioGRID	PubMed	Synthetic Lethality
BioGRID:36247	BioGRID:34342	GPD2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32388	GPI8	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:31873	GUD1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33166	GUP1	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:31115	HAC1	BioGRID	PubMed	Phenotypic Suppression
BioGRID:36247	BioGRID:33831	HOC1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31384	HOG1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33895	HOM6	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36830	ILV1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33772	ILV3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33607	INO1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:34321	IRA2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32067	KCS1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31528	LCB5	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35972	LEA1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33632	LSM1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:36247	BioGRID:33962	LST4	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35819	LST8	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35532	MCK1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32049	MED2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:34944	MET30	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31592	NMA1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35765	OCA2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31103	OTU1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:31052	PAT1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:36247	BioGRID:33628	PBS2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35726	PHO23	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35082	PHO84	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35945	PLC1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33238	PMA1	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:34120	PRP40	BioGRID	PubMed	Protein-peptide
BioGRID:36247	BioGRID:33816	PTK2	BioGRID	PubMed	Biochemical Activity
BioGRID:36247	BioGRID:36819	PTP3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35238	RIM9	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33635	RPE1	BioGRID	PubMed	Positive Genetic; Synthetic Lethality
BioGRID:36247	BioGRID:36458	RPN1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:36634	RPN10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:31152	RPN11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:34513	RPT5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:36869	RSP5	BioGRID	PubMed	Protein-peptide; Reconstituted Complex
BioGRID:36247	BioGRID:31451	SAM1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36388	SBP1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:36247	BioGRID:33149	SEH1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33591	SET2	BioGRID	PubMed	Protein-peptide
BioGRID:36247	BioGRID:34005	SHE2	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:36247	BioGRID:31833	SHR3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:34400	SIN3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32566	SLF1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:32779	SLM4	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31139	SMC1	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:32561	SMT3	BioGRID	PubMed	Affinity Capture-MS; PCA
BioGRID:36247	BioGRID:35270	SNO1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35501	SNO4	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33860	SOD1	BioGRID	PubMed	Dosage Rescue; Phenotypic Suppression
BioGRID:36247	BioGRID:36473	SRB2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35627	SSB2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:32345	SSD1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36987	SUS1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33585	SWI3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:33139	TAF6	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35090	TAF8	BioGRID	PubMed	Positive Genetic
BioGRID:36247	BioGRID:31616	TAL1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:36247	BioGRID:32772	TAT1	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31033	THR4	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36247	TKL1	BioGRID	PubMed	Reconstituted Complex
BioGRID:36247	BioGRID:32819	TKL2	BioGRID	PubMed	Affinity Capture-MS; Negative Genetic; Synthetic Growth Defect; Synthetic Lethality
BioGRID:36247	BioGRID:33954	TRP3	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:31215	UBI4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:35647	UBP10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:35609	URE2	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:36319	URN1	BioGRID	PubMed	Protein-peptide
BioGRID:36247	BioGRID:31137	WWM1	BioGRID	PubMed	Protein-peptide
BioGRID:36247	BioGRID:32565	YDR514C	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:34999	YIR007W	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:36409	YLF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:36247	BioGRID:35651	YNL181W	BioGRID	PubMed	Negative Genetic
BioGRID:36247	BioGRID:35598	ZWF1	BioGRID	PubMed	Synthetic Growth Defect; Synthetic Lethality

Pathways from BioSystems

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Biosynthesis of amino acids, organism-specific biosystem (from KEGG)
Biosynthesis of amino acids, organism-specific biosystem
Biosynthesis of amino acids
Biosynthesis of amino acids, conserved biosystem (from KEGG)
Biosynthesis of amino acids, conserved biosystem
Biosynthesis of amino acids
Biosynthesis of secondary metabolites, organism-specific biosystem (from KEGG)
Biosynthesis of secondary metabolites, organism-specific biosystem
Biosynthesis of secondary metabolites
Deoxyribose phosphate metabolism, organism-specific biosystem (from WikiPathways)
Deoxyribose phosphate metabolism, organism-specific biosystem
Deoxyribose phosphate metabolism
Non-Oxidative Branch of the Pentose Pathway, organism-specific biosystem (from WikiPathways)
Non-Oxidative Branch of the Pentose Pathway, organism-specific biosystem
Non-Oxidative Branch of the Pentose Pathway
Pentose Phosphate Pathway, organism-specific biosystem (from WikiPathways)
Pentose Phosphate Pathway, organism-specific biosystemThe pentose phosphate pathway is important for generating NADPH, which is a source of reducing energy, as well as a variety of sugar molecules that are required for the biosynthesis of nucleic acid...
Pentose Phosphate Pathway 2, organism-specific biosystem (from WikiPathways)
Pentose Phosphate Pathway 2, organism-specific biosystemPentose Phosphate Pathway in Saccharomyces cerevisiae created for the course Biochemie (BT-VT03)
Pentose phosphate pathway, organism-specific biosystem (from KEGG)
Pentose phosphate pathway, organism-specific biosystemThe pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathwa...
Pentose phosphate pathway, conserved biosystem (from KEGG)
Pentose phosphate pathway, conserved biosystemThe pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathwa...
Pentose phosphate pathway (Pentose phosphate cycle), organism-specific biosystem (from KEGG)
Pentose phosphate pathway (Pentose phosphate cycle), organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Pentose phosphate pathway (Pentose phosphate cycle), conserved biosystem (from KEGG)
Pentose phosphate pathway (Pentose phosphate cycle), conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P, organism-specific biosystem (from KEGG)
Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P, conserved biosystem (from KEGG)
Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
Principle Pathways of Carbon Metabolism, organism-specific biosystem (from WikiPathways)
Principle Pathways of Carbon Metabolism, organism-specific biosystem
Principle Pathways of Carbon Metabolism
Ribose and Deoxyribose Phosphate Metabolism, organism-specific biosystem (from WikiPathways)
Ribose and Deoxyribose Phosphate Metabolism, organism-specific biosystem
Ribose and Deoxyribose Phosphate Metabolism
Xylulose-monophosphate cycle, organism-specific biosystem (from WikiPathways)
Xylulose-monophosphate cycle, organism-specific biosystem
Xylulose-monophosphate cycle
pentose phosphate pathway, organism-specific biosystem (from BIOCYC)
pentose phosphate pathway, organism-specific biosystem
pentose phosphate pathway
pentose phosphate pathway (non-oxidative branch), organism-specific biosystem (from BIOCYC)
pentose phosphate pathway (non-oxidative branch), organism-specific biosystem
pentose phosphate pathway (non-oxidative branch)

General gene information

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Homology

Homologs of the TKL1 gene: The TKL1 gene is conserved in S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
catalytic activity	IEA Inferred from Electronic Annotation more info
metal ion binding	IEA Inferred from Electronic Annotation more info
transferase activity	IEA Inferred from Electronic Annotation more info
transketolase activity	IEA Inferred from Electronic Annotation more info
transketolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
transketolase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
metabolic process	IEA Inferred from Electronic Annotation more info
pentose-phosphate shunt	IMP Inferred from Mutant Phenotype more info	PubMed
pentose-phosphate shunt	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Names: transketolase TKL1

NP_015399.1

EC 2.2.1.1

Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4

Range

692796..694838, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184171.1 → NP_015399.1 transketolase TKL1 [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P23254

Conserved Domains (4) summary

cd02012
Location:14 – 276
Blast Score: 926

TPP_TK; Thiamine pyrophosphate (TPP) family, Transketolase (TK) subfamily, TPP-binding module; TK catalyzes the transfer of a two-carbon unit from ketose phosphates to aldose phosphates. In heterotrophic organisms, TK provides a link between glycolysis and the ...

cd07033
Location:360 – 528
Blast Score: 484

TPP_PYR_DXS_TK_like; Pyrimidine (PYR) binding domain of 1-deoxy-D-xylulose-5-phosphate synthase (DXS), transketolase (TK), and related proteins.

TIGR00232
Location:9 – 664
Blast Score: 2845

tktlase_bact; transketolase, bacterial and yeast

pfam02780
Location:546 – 656
Blast Score: 216

Transketolase_C; Transketolase, C-terminal domain