CYB2 L-lactate dehydrogenase (cytochrome) [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CYB2
Gene description: L-lactate dehydrogenase (cytochrome)
Primary source: SGD:S000004518
Locus tag: YML054C
See related: AllianceGenome:SGD:S000004518
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: FCB2
Summary: Enables L-lactate dehydrogenase (cytochrome) activity. Involved in lactate metabolic process. Located in mitochondrial intermembrane space. Orthologous to several human genes including HAO1 (hydroxyacid oxidase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (165533..167308, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The present results support the idea that the heme domain mobility is an inherent component of the Fcb2 functioning.
Title: Structural evidence for the functional importance of the heme domain mobility in flavocytochrome b2.
Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.
Title: Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition.
separately engineered flavodehydrogenase domain produces superoxide anion in its slow reaction with oxygen. This reaction also takes place in the holoenzyme when oxygen is the sole electron acceptor, because the heme domain autoxidation is also slow.
Title: Flavocytochrome b2: reactivity of its flavin with molecular oxygen.
The combination of an altered active site and the small kinetic isotope effects is consistent with the slowest step in turnover being a conformational change involving a conformation in which lactate is bound unproductively.
Title: Mechanistic and structural studies of H373Q flavocytochrome b2: effects of mutating the active site base.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5S559 (e-PCR)
- UniSTS:148320

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables L-lactate dehydrogenase (cytochrome) activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-lactate dehydrogenase (cytochrome) activity	IDA Inferred from Direct Assay more info	PubMed
enables L-lactate dehydrogenase (cytochrome) activity	IEA Inferred from Electronic Annotation more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in lactate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in lactate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial intermembrane space	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial intermembrane space	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in respirasome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: L-lactate dehydrogenase (cytochrome)

NP_013658.1

EC 1.1.2.3

Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

165533..167308 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182412.1 → NP_013658.1 TPA: L-lactate dehydrogenase (cytochrome) [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013658.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZC0, P00175

UniProtKB/TrEMBL

A0A6A5PPC6, A6ZM12, B3LLK2, C7GME9, C8ZEF3, G2WK02, N1NZC2

Conserved Domains (2) summary

COG5274
Location:34 → 187

CYB5; Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism]

cd02922
Location:206 → 555

FCB2_FMN; Flavocytochrome b2 (FCB2) FMN-binding domain. FCB2 (AKA L-lactate:cytochrome c oxidoreductase) is a respiratory enzyme located in the intermembrane space of fungal mitochondria which catalyzes the oxidation of L-lactate to pyruvate. FCB2 also ...