VMA4 Vma4p [Saccharomyces cerevisiae S288c] - Gene

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Summary

Gene symbol: VMA4
Gene description: Vma4p
Primary source: SGD:S000005859
Locus tag: YOR332W
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

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Genomic context

Sequence :: Chromosome: XV; NC_001147.6 (943656..944357)

See VMA4 in MapViewer

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

new structural details of subunit E are discussed in the light of the structural arrangements of this subunit inside the V- and VV ATPase.
Title: Structural elements of the C-terminal domain of subunit E (E₁₃₃₋₂₂₂) from the Saccharomyces cerevisiae V₁V₀ ATPase determined by solution NMR spectroscopy.
NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase.
Title: NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase.
These results suggest that subunit E, especially its amino-terminal domain, plays a pertinent role in the assembly of V-ATPase subunits in vacuolar membranes from mice and yeast.
Title: Defective assembly of a hybrid vacuolar H(+)-ATPase containing the mouse testis-specific E1 isoform and yeast subunits.
an increase in the level of the TIM23 translocase associated with the cytochrome bc1-COX supercomplex is observed in the absence of Su e and Su g
Title: The F1F0-ATP synthase complex influences the assembly state of the cytochrome bc1-cytochrome oxidase supercomplex and its association with the TIM23 machinery.
Mutational analysis of V-ATPase indicates that subunit E could communicate cellular changes to the catalytic sites upon binding to regulatory proteins which interact with subunit E as a means of regulating V-ATPase function.
Title: Mutational analysis of the stator subunit E of the yeast V-ATPase.
Protein interaction studies show that the N-terminal region of Vma4 (subunit E) is a key factor not only in the stator structure of the vacuolar ATPase rotary molecular motor, but also in mediating interactions with putative regulatory subunits.
Title: Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H+-ATPase.

Submit: New GeneRIF Correction

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
NP_014977.1	NP_012567.1	RAV1	BIND	PubMed	Rav1 interacts with Vma4.
NP_014977.1	NP_010488.1	RAV2	BIND	PubMed	Rav2 interacts with Vma4.
NP_014977.1	NP_011905.1	VMA10	BIND	PubMed	Vma10 interacts with the amino terminus of Vma4.
NP_014977.1	NP_015361.1	VMA13	BIND	PubMed	The amino terminus of Vma4 (subunit E) interacts with Vma13 (subunit H). This interaction was modeled on a demonstrated interaction between human Vma4 and human Vma13.
NP_014977.1	NP_012843.1	VMA5	BIND	PubMed	Vma5 interacts with hydrophobic residues in the amino terminus of Vma4.
BioGRID:34716	BioGRID:31107	ACT1	BioGRID	PubMed	Synthetic Haploinsufficiency
BioGRID:34716	BioGRID:36440	ARD1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:31123	BUD27	BioGRID	PubMed	PCA
BioGRID:34716	BioGRID:31166	CMK1	BioGRID	PubMed	Biochemical Activity
BioGRID:34716	BioGRID:33931	CNB1	BioGRID	PubMed	Synthetic Lethality
BioGRID:34716	BioGRID:31961	DUN1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:31122	EPL1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:34635	ESA1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:33226	GET1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:36828	GET2	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:35584	GIS2	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-RNA
BioGRID:34716	BioGRID:36877	GLC7	BioGRID	PubMed	Synthetic Lethality
BioGRID:34716	BioGRID:35980	HRR25	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:35364	HSC82	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:35923	HSP82	BioGRID	PubMed	Affinity Capture-MS; Synthetic Growth Defect; Two-hybrid
BioGRID:34716	BioGRID:34031	LAP4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:36811	MOT2	BioGRID	PubMed	Synthetic Lethality
BioGRID:34716	BioGRID:33129	NAB2	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:34716	BioGRID:32018	NAT1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:33819	NTA1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:34997	PAN1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:32817	RAD16	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:33786	RAV1	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Two-hybrid
BioGRID:34716	BioGRID:32253	RAV2	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34716	BioGRID:36277	RPC40	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:36634	RPN10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:31152	RPN11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:32035	RPN4	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:31439	SEC10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:35234	SEN15	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:34437	SHE4	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:32385	SKP1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:34716	BioGRID:31299	SNF7	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:34716	BioGRID:35230	STV1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:34716	BioGRID:33246	SWC4	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34716	BioGRID:31215	UBI4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34716	BioGRID:31859	VMA1	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-fractionation; Reconstituted Complex
BioGRID:34716	BioGRID:36471	VMA10	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-fractionation; Far Western; Reconstituted Complex; Two-hybrid
BioGRID:34716	BioGRID:36214	VMA13	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification; Synthetic Rescue; Two-hybrid
BioGRID:34716	BioGRID:32828	VMA2	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification; Reconstituted Complex
BioGRID:34716	BioGRID:34716	VMA4	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:34716	BioGRID:34052	VMA5	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34716	BioGRID:31705	VMA6	BioGRID	PubMed	Two-hybrid
BioGRID:34716	BioGRID:33262	VMA7	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34716	BioGRID:36678	VMA8	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification
BioGRID:34716	BioGRID:34659	VPH1	BioGRID	PubMed	Affinity Capture-Western; Co-fractionation
BioGRID:34716	BioGRID:34091	VPS24	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:34716	BioGRID:33858	VPS25	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:34716	BioGRID:31675	VPS36	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:34716	BioGRID:36525	YNG2	BioGRID	PubMed	Synthetic Growth Defect

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General gene information

Homology

Homologs of the VMA4 gene: The VMA4 gene is conserved in human, chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, M.grisea, N.crassa, A.thaliana, rice, and P.falciparum.

Pathways from BioSystems

Insulin receptor recycling, organism-specific biosystem (from REACTOME)
Insulin receptor recycling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolic pathways, organism-specific biosystem (from KEGG)
Metabolic pathways, organism-specific biosystem
Metabolic pathways
Oxidative phosphorylation, organism-specific biosystem (from KEGG)
Oxidative phosphorylation, organism-specific biosystem
Oxidative phosphorylation
Oxidative phosphorylation, conserved biosystem (from KEGG)
Oxidative phosphorylation, conserved biosystem
Oxidative phosphorylation
Phagosome, organism-specific biosystem (from KEGG)
Phagosome, organism-specific biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
Phagosome, conserved biosystem (from KEGG)
Phagosome, conserved biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by Insulin receptor, organism-specific biosystem (from REACTOME)
Signaling by Insulin receptor, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
proton-transporting ATPase activity, rotational mechanism	IEA Inferred from Electronic Annotation more info
proton-transporting ATPase activity, rotational mechanism	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
ATP hydrolysis coupled proton transport	IEA Inferred from Electronic Annotation more info
ion transport	IEA Inferred from Electronic Annotation more info
proton transport	IEA Inferred from Electronic Annotation more info
transport	IEA Inferred from Electronic Annotation more info
vacuolar acidification	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
fungal-type vacuole membrane	TAS Traceable Author Statement more info	PubMed
integral to membrane	ISM Inferred from Sequence Model more info	PubMed
membrane	IEA Inferred from Electronic Annotation more info
proton-transporting two-sector ATPase complex, catalytic domain	IEA Inferred from Electronic Annotation more info
vacuolar membrane	IEA Inferred from Electronic Annotation more info
vacuolar proton-transporting V-type ATPase, V1 domain	IDA Inferred from Direct Assay more info	PubMed
vacuole	IEA Inferred from Electronic Annotation more info

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General protein information

Names: Vma4p

NP_014977.1

EC 3.6.3.14

Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6

Range

943656..944357

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183752.1 → NP_014977.1 Vma4p [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P22203

Conserved Domains (2) summary

pfam01991
Location:23 – 223
Blast Score: 448

vATP-synt_E; ATP synthase (E/31 kDa) subunit

cl07975
Location:14 – 225
Blast Score: 231

YMF19; Plant ATP synthase F0