RPB8 Rpb8p [Saccharomyces cerevisiae S288c] - Gene

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Summary

Gene symbol: RPB8
Gene description: Rpb8p
Primary source: SGD:S000005750
Locus tag: YOR224C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

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Genomic context

Location :: chromosome: XV

Sequence :: Chromosome: XV; NC_001147.6 (760825..761265, complement)

See RPB8 in MapViewer

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters.
Title: Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.

Submit: New GeneRIF Correction

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:34618	BioGRID:36261	ASR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35957	CET1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:34618	BioGRID:35002	DSN1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34353	GAL11	BioGRID	PubMed	PCA
BioGRID:34618	BioGRID:32710	HHF1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35797	HHF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:32276	HTB1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:32700	HTB2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:31947	IWR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:31369	KIN2	BioGRID	PubMed	Reconstituted Complex
BioGRID:34618	BioGRID:36517	KSP1	BioGRID	PubMed	Biochemical Activity
BioGRID:34618	BioGRID:35532	MCK1	BioGRID	PubMed	Biochemical Activity
BioGRID:34618	BioGRID:33129	NAB2	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:34618	BioGRID:33331	NNF2	BioGRID	PubMed	Two-hybrid
BioGRID:34618	BioGRID:33693	NUP82	BioGRID	PubMed	Two-hybrid
BioGRID:34618	BioGRID:35101	ORC1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34120	PRP40	BioGRID	PubMed	Protein-peptide
BioGRID:34618	BioGRID:34602	RET1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33820	RPA12	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:36188	RPA135	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34724	RPA190	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34618	BioGRID:34723	RPA43	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34618	BioGRID:35591	RPA49	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34605	RPB10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34399	RPB11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34547	RPB2	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34618	BioGRID:34967	RPB3	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification
BioGRID:34618	BioGRID:33617	RPB4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:32853	RPB5	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34618	BioGRID:32464	RPB7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33178	RPB9	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification
BioGRID:34618	BioGRID:36577	RPC10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:32100	RPC11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33744	RPC17	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35712	RPC19	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33992	RPC25	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35678	RPC31	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35829	RPC34	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34157	RPC37	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:36277	RPC40	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:31911	RPC53	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:36362	RPC82	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:34225	RPL40B	BioGRID	PubMed	Dosage Rescue
BioGRID:34618	BioGRID:31921	RPO21	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Co-purification; Two-hybrid
BioGRID:34618	BioGRID:36359	RPO26	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Dosage Rescue
BioGRID:34618	BioGRID:34512	RPO31	BioGRID	PubMed	Affinity Capture-MS; Two-hybrid
BioGRID:34618	BioGRID:36869	RSP5	BioGRID	PubMed	Affinity Capture-MS; Protein-peptide
BioGRID:34618	BioGRID:36888	RTR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:30987	SAT4	BioGRID	PubMed	Biochemical Activity
BioGRID:34618	BioGRID:33591	SET2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:32872	SMY2	BioGRID	PubMed	Dosage Rescue
BioGRID:34618	BioGRID:31147	SPB4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35159	SPT5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:36756	SRB4	BioGRID	PubMed	Co-purification
BioGRID:34618	BioGRID:33347	SRB5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:30946	SRO9	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:34618	BioGRID:32345	SSD1	BioGRID	PubMed	Dosage Rescue
BioGRID:34618	BioGRID:36052	TAF14	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33438	TFG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:33249	TFG2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35981	TPK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:31215	UBI4	BioGRID	PubMed	Dosage Rescue
BioGRID:34618	BioGRID:31907	YDL156W	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34618	BioGRID:35960	YPL225W	BioGRID	PubMed	Affinity Capture-MS

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General gene information

Homology

Homologs of the RPB8 gene: The RPB8 gene is conserved in human, chimpanzee, , dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, , , N.crassa, A.thaliana, and rice.

Pathways from BioSystems

DNA Repair, organism-specific biosystem (from REACTOME)
DNA Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Dual incision reaction in TC-NER, organism-specific biosystem (from REACTOME)
Dual incision reaction in TC-NER, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Eukaryotic Transcription Initiation, organism-specific biosystem (from WikiPathways)
Eukaryotic Transcription Initiation, organism-specific biosystemIn eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the pre...
Formation of the Early Elongation Complex, organism-specific biosystem (from REACTOME)
Formation of the Early Elongation Complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Formation of transcription-coupled NER (TC-NER) repair complex, organism-specific biosystem (from REACTOME)
Formation of transcription-coupled NER (TC-NER) repair complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Gene Expression, organism-specific biosystem (from REACTOME)
Gene Expression, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolic pathways, organism-specific biosystem (from KEGG)
Metabolic pathways, organism-specific biosystem
Metabolic pathways
MicroRNA (miRNA) Biogenesis, organism-specific biosystem (from REACTOME)
MicroRNA (miRNA) Biogenesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Nucleotide Excision Repair, organism-specific biosystem (from REACTOME)
Nucleotide Excision Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem (from REACTOME)
Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Purine metabolism, organism-specific biosystem (from KEGG)
Purine metabolism, organism-specific biosystem
Purine metabolism
Purine metabolism, conserved biosystem (from KEGG)
Purine metabolism, conserved biosystem
Purine metabolism
Pyrimidine metabolism, organism-specific biosystem (from KEGG)
Pyrimidine metabolism, organism-specific biosystem
Pyrimidine metabolism
Pyrimidine metabolism, conserved biosystem (from KEGG)
Pyrimidine metabolism, conserved biosystem
Pyrimidine metabolism
RNA Pol II CTD phosphorylation and interaction with CE, organism-specific biosystem (from REACTOME)
RNA Pol II CTD phosphorylation and interaction with CE, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase I Promoter Clearance, organism-specific biosystem (from REACTOME)
RNA Polymerase I Promoter Clearance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase I Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase I Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase I Transcription Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase I Transcription Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Pre-transcription Events, organism-specific biosystem (from REACTOME)
RNA Polymerase II Pre-transcription Events, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Promoter Escape, organism-specific biosystem (from REACTOME)
RNA Polymerase II Promoter Escape, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Elongation, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Abortive And Retractive Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase III Abortive And Retractive Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Chain Elongation, organism-specific biosystem (from REACTOME)
RNA Polymerase III Chain Elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription Initiation From Type 1 Promoter, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription Initiation From Type 1 Promoter, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription Initiation From Type 2 Promoter, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription Initiation From Type 2 Promoter, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription Initiation From Type 3 Promoter, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription Initiation From Type 3 Promoter, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase III Transcription Termination, organism-specific biosystem (from REACTOME)
RNA Polymerase III Transcription Termination, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA polymerase, organism-specific biosystem (from KEGG)
RNA polymerase, organism-specific biosystem
RNA polymerase
RNA polymerase, conserved biosystem (from KEGG)
RNA polymerase, conserved biosystem
RNA polymerase
RNA polymerase I, eukaryotes, organism-specific biosystem (from KEGG)
RNA polymerase I, eukaryotes, organism-specific biosystemStructural complex; Genetic information processing; RNA polymerase
RNA polymerase II, eukaryotes, organism-specific biosystem (from KEGG)
RNA polymerase II, eukaryotes, organism-specific biosystemStructural complex; Genetic information processing; RNA polymerase
RNA polymerase III, eukaryotes, organism-specific biosystem (from KEGG)
RNA polymerase III, eukaryotes, organism-specific biosystemStructural complex; Genetic information processing; RNA polymerase
Regulatory RNA pathways, organism-specific biosystem (from REACTOME)
Regulatory RNA pathways, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Transcription-coupled NER (TC-NER), organism-specific biosystem (from REACTOME)
Transcription-coupled NER (TC-NER), organism-specific biosystemcomputationally inferred pathway (not manually curated)
mRNA Capping, organism-specific biosystem (from REACTOME)
mRNA Capping, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
contributes_to DNA-directed RNA polymerase activity	IC Inferred by Curator more info	PubMed
contributes_to DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
DNA-directed RNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
tRNA transcription from RNA polymerase III promoter	IDA Inferred from Direct Assay more info	PubMed
transcription from RNA polymerase I promoter	IPI Inferred from Physical Interaction more info	PubMed
transcription from RNA polymerase II promoter	IPI Inferred from Physical Interaction more info	PubMed
transcription from RNA polymerase III promoter	IGI Inferred from Genetic Interaction more info	PubMed
transcription from RNA polymerase III promoter	IPI Inferred from Physical Interaction more info	PubMed
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
DNA-directed RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
DNA-directed RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
DNA-directed RNA polymerase III complex	IDA Inferred from Direct Assay more info	PubMed
DNA-directed RNA polymerase III complex	TAS Traceable Author Statement more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info

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General protein information

Names: Rpb8p

NP_014867.1

RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6

Range

760825..761265, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183643.1 → NP_014867.1 Rpb8p [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P20436

Conserved Domains (1) summary

cl02716
Location:2 – 146
Blast Score: 453

RNA_pol_Rpb8; RNA polymerase Rpb8