TIF1 Tif1p [ Saccharomyces cerevisiae S288c ]

Gene ID: 853933, updated on 31-Jan-2012

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Summary

Gene symbol: TIF1
Gene description: Tif1p
Primary source: SGD:S000001767
Locus tag: YKR059W
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

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Genomic context

Sequence :: Chromosome: XI; NC_001143.9 (554987..556174)

See TIF1 in MapViewer

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glucose depletion inhibits translation initiation via eukaryotic initiation factor 4a loss and subsequent 48S preinitiation complex accumulation
Title: Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated.
structure of the complex formed by yeast eIF4G's middle domain and full-length eIF4A at 2.6-A resolution
Title: Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions.

Submit: New GeneRIF Correction

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:34190	BioGRID:32931	ABD1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32268	AHA1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:34913	AIR1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:36693	ANP1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33145	ARC1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31358	ARP6	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32159	ARX1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32897	BEM1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36930	BMH1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32157	BMH2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:35876	BRE5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:34325	BRX1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34429	BUB3	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:31123	BUD27	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34190	BioGRID:36910	BUR6	BioGRID	PubMed	Dosage Rescue
BioGRID:34190	BioGRID:32859	CDC28	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:31579	CDC3	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:34278	CDC33	BioGRID	PubMed	Affinity Capture-MS; Synthetic Lethality
BioGRID:34190	BioGRID:32038	CDC7	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34190	BioGRID:31676	CDC73	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:33217	CGR1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36668	CIN8	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34190	BioGRID:34817	COX3	BioGRID	PubMed	Dosage Rescue
BioGRID:34190	BioGRID:33472	CRM1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32106	DBF4	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34190	BioGRID:34447	DBP5	BioGRID	PubMed	Dosage Rescue
BioGRID:34190	BioGRID:35708	DCP2	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:34599	DED1	BioGRID	PubMed	Negative Genetic; Synthetic Lethality
BioGRID:34190	BioGRID:36448	DED81	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31148	DEG1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31903	DHH1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:36712	EAF5	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:35690	EAF7	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33919	EAP1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36392	ECM29	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:34529	EFT1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32446	EFT2	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32805	FES1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33503	GCN5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:34740	GDS1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:35584	GIS2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32388	GPI8	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33063	HOS2	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34391	HRD1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33845	JSN1	BioGRID	PubMed	Protein-RNA
BioGRID:34190	BioGRID:36056	KAP120	BioGRID	PubMed	Positive Genetic; Two-hybrid
BioGRID:34190	BioGRID:36855	KAP123	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:36620	KOG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:35695	KRE33	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:30927	KRR1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:36871	LCP5	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34518	LEO1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:36590	LIN1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32495	LRS4	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33632	LSM1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34241	MED7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33076	MPT5	BioGRID	PubMed	Protein-RNA
BioGRID:34190	BioGRID:35256	NAM7	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36511	NMD2	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32040	NOP1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:35715	NOP15	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31467	NOP56	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34065	NUP100	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32624	NUP170	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:35040	NUP188	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36918	PAB1	BioGRID	PubMed	Synthetic Rescue
BioGRID:34190	BioGRID:32647	PIN4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32598	PKC1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32867	POP7	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32680	POP8	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31217	PRP19	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:36745	PRP22	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:34746	PRT1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:34357	PSK2	BioGRID	PubMed	Biochemical Activity
BioGRID:34190	BioGRID:36219	PUF2	BioGRID	PubMed	Protein-RNA
BioGRID:34190	BioGRID:31238	PUF3	BioGRID	PubMed	Protein-RNA
BioGRID:34190	BioGRID:33232	PUF4	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32148	RLI1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:34212	RPF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:31553	RPS30A	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:34674	RRP36	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:34404	RRP6	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:35441	SAP30	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32420	SEM1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32807	SIF2	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32385	SKP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:31408	SLS1	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32561	SMT3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:36586	SPO12	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32783	SPT7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32344	SRP101	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:31882	SSB1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33763	SUI2	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32225	SUP35	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:32457	SXM1	BioGRID	PubMed	Positive Genetic
BioGRID:34190	BioGRID:36168	TAE2	BioGRID	PubMed	Dosage Rescue; Synthetic Rescue
BioGRID:34190	BioGRID:32199	TAF12	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32895	TAF5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33139	TAF6	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:117003	TARDBP	BioGRID	PubMed	Synthetic Rescue
BioGRID:34190	BioGRID:33619	TIF2	BioGRID	PubMed	Affinity Capture-MS; Negative Genetic
BioGRID:34190	BioGRID:36336	TIF3	BioGRID	PubMed	Dosage Rescue; Synthetic Lethality
BioGRID:34190	BioGRID:33414	TIF4631	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Negative Genetic; Reconstituted Complex; Synthetic Lethality
BioGRID:34190	BioGRID:33199	TIF4632	BioGRID	PubMed	Dosage Rescue; Reconstituted Complex
BioGRID:34190	BioGRID:36218	TIF5	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:32827	TPS1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:31693	TSR2	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:31215	UBI4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:36901	UBP3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:35401	UBP8	BioGRID	PubMed	Affinity Capture-MS; Negative Genetic
BioGRID:34190	BioGRID:34122	UFD4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:33315	UPF3	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33626	URA2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32658	URA7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:32076	YDR026C	BioGRID	PubMed	Negative Genetic
BioGRID:34190	BioGRID:33299	YGR054W	BioGRID	PubMed	Affinity Capture-MS; Negative Genetic
BioGRID:34190	BioGRID:36409	YLF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34190	BioGRID:35452	ZDS1	BioGRID	PubMed	Dosage Rescue

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General gene information

Pathways from BioSystems

Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem (from REACTOME)
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cap-dependent Translation Initiation, organism-specific biosystem (from REACTOME)
Cap-dependent Translation Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Eukaryotic Translation Initiation, organism-specific biosystem (from REACTOME)
Eukaryotic Translation Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Gene Expression, organism-specific biosystem (from REACTOME)
Gene Expression, organism-specific biosystemcomputationally inferred pathway (not manually curated)
L13a-mediated translational silencing of Ceruloplasmin expression, organism-specific biosystem (from REACTOME)
L13a-mediated translational silencing of Ceruloplasmin expression, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of proteins, organism-specific biosystem (from REACTOME)
Metabolism of proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA transport, organism-specific biosystem (from KEGG)
RNA transport, organism-specific biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
RNA transport, conserved biosystem (from KEGG)
RNA transport, conserved biosystemRNA transport from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes (NPCs) ...
Ribosomal scanning and start codon recognition, organism-specific biosystem (from REACTOME)
Ribosomal scanning and start codon recognition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translation, organism-specific biosystem (from REACTOME)
Translation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
eIF4F complex, organism-specific biosystem (from KEGG)
eIF4F complex, organism-specific biosystemStructural complex; Genetic information processing; RNA processing

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
ATP-dependent RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
ATP-dependent RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
ATP-dependent helicase activity	IEA Inferred from Electronic Annotation more info
RNA binding	IEA Inferred from Electronic Annotation more info
helicase activity	IEA Inferred from Electronic Annotation more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
nucleic acid binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
translation initiation factor activity	IEA Inferred from Electronic Annotation more info
translation initiation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
translation	IEA Inferred from Electronic Annotation more info
translational initiation	IDA Inferred from Direct Assay more info	PubMed
translational initiation	IEA Inferred from Electronic Annotation more info
translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
eukaryotic translation initiation factor 4F complex	IGI Inferred from Genetic Interaction more info	PubMed
eukaryotic translation initiation factor 4F complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
ribosome	TAS Traceable Author Statement more info	PubMed

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General protein information

Names: Tif1p

NP_012985.1

EC 3.6.1.-

Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9

Range

554987..556174

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179849.1 → NP_012985.1 Tif1p [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P10081

Conserved Domains (3) summary

cd00268
Location:24 – 223
Blast Score: 646

DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...

cd00079
Location:234 – 346
Blast Score: 258

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

PTZ00424
Location:10 – 394
Blast Score: 1364

PTZ00424; helicase 45; Provisional