VMA5 Vma5p [Saccharomyces cerevisiae S288c] - Gene

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Summary

Gene symbol: VMA5
Gene description: Vma5p
Primary source: SGD:S000001563
Locus tag: YKL080W
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CSL5; VAT3

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Genomic context

Sequence :: Chromosome: XI; NC_001143.9 (285030..286208)

See VMA5 in MapViewer

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Domain characterization and interaction of the yeast vacuolar ATPase subunit C with the peripheral stator stalk subunits E and G
Title: Domain characterization and interaction of the yeast vacuolar ATPase subunit C with the peripheral stator stalk subunits E and G.
Nucleotide occupancy by subunit C, followed by conformational changes of this subunit has shed light on the mechanism of V-ATPase regulation.
Title: Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases.
Vma5 (subunit C) expressed as a glutathione-S-transferase fusion protein in Escherichia coli is able to interact strongly with Vma4 (subunit E) expressed in a cell-free system.
Title: Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H+-ATPase.
The data show that subunit C is binding at the interface of the ATPase and proton channel, opposite from another stalk density previously identified as subunit H.
Title: Localization of subunit C (Vma5p) in the yeast vacuolar ATPase by immuno electron microscopy.
VMA5p subunit C localizes to the interface of the V1 and V0 domains, which is consistent with a role for this subunit in controlling assembly of the V-ATPase complex
Title: Cysteine-mediated cross-linking indicates that subunit C of the V-ATPase is in close proximity to subunits E and G of the V1 domain and subunit a of the V0 domain.
Expression, crystallization and phasing of vacuolar H(+)-ATPase subunit C (Vma5p) of Saccharomyces cerevisiae
Title: Expression, crystallization and phasing of vacuolar H(+)-ATPase subunit C (Vma5p) of Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
NP_012843.1	NP_014977.1	VMA4	BIND	PubMed	Vma5 interacts with hydrophobic residues in the amino terminus of Vma4.
BioGRID:34052	BioGRID:31422	ACS2	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:31107	ACT1	BioGRID	PubMed	Synthetic Haploinsufficiency
BioGRID:34052	BioGRID:33761	APL1	BioGRID	PubMed	Positive Genetic
BioGRID:34052	BioGRID:31853	ARF1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:33675	ARP4	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:33427	CBP4	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:33135	CDC20	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:31724	CDC24	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:33931	CNB1	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:32462	DIT2	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:31122	EPL1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:34635	ESA1	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:31884	GCS1	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:33226	GET1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:36828	GET2	BioGRID	PubMed	Synthetic Lethality
BioGRID:34052	BioGRID:31962	GET3	BioGRID	PubMed	PCA
BioGRID:34052	BioGRID:36090	GLR1	BioGRID	PubMed	PCA
BioGRID:34052	BioGRID:33166	GUP1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:34469	GYP1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:32227	HMO1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:32883	MBA1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:34885	MOB1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:32950	MTC4	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:34942	NEO1	BioGRID	PubMed	Dosage Rescue
BioGRID:34052	BioGRID:36507	OSH3	BioGRID	PubMed	Positive Genetic
BioGRID:34052	BioGRID:32681	PEP1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:33701	PEP8	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:33087	PMR1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:31831	PRR2	BioGRID	PubMed	Biochemical Activity
BioGRID:34052	BioGRID:36623	PTH1	BioGRID	PubMed	Positive Genetic
BioGRID:34052	BioGRID:33816	PTK2	BioGRID	PubMed	Biochemical Activity
BioGRID:34052	BioGRID:33189	RAD6	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:33786	RAV1	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:34052	BioGRID:32253	RAV2	BioGRID	PubMed	PCA; Two-hybrid
BioGRID:34052	BioGRID:34844	RPL40A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:36458	RPN1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:36634	RPN10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:31152	RPN11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:32443	RPP2B	BioGRID	PubMed	Positive Genetic
BioGRID:34052	BioGRID:32629	RPS8A	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:35234	SEN15	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:32011	SIT4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:36152	SKS1	BioGRID	PubMed	Biochemical Activity
BioGRID:34052	BioGRID:31147	SPB4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:33873	STE24	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:35230	STV1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:34052	BioGRID:32182	SWF1	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:35102	TEM1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:34385	TLG2	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:34398	TOP1	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:34052	BioGRID:31750	TPD3	BioGRID	PubMed	PCA
BioGRID:34052	BioGRID:32166	TRS85	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:31215	UBI4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:31859	VMA1	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34052	BioGRID:36471	VMA10	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34052	BioGRID:36214	VMA13	BioGRID	PubMed	Synthetic Rescue
BioGRID:34052	BioGRID:32828	VMA2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:34716	VMA4	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:34052	BioGRID:31705	VMA6	BioGRID	PubMed	PCA
BioGRID:34052	BioGRID:33262	VMA7	BioGRID	PubMed	PCA
BioGRID:34052	BioGRID:36678	VMA8	BioGRID	PubMed	Affinity Capture-MS
BioGRID:34052	BioGRID:34659	VPH1	BioGRID	PubMed	Affinity Capture-Western; Co-fractionation
BioGRID:34052	BioGRID:36439	VPS29	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:33606	VPS35	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:36113	YPL066W	BioGRID	PubMed	Positive Genetic
BioGRID:34052	BioGRID:36259	YPR089W	BioGRID	PubMed	Negative Genetic
BioGRID:34052	BioGRID:36320	YPR153W	BioGRID	PubMed	Negative Genetic

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General gene information

Pathways from BioSystems

Insulin receptor recycling, organism-specific biosystem (from REACTOME)
Insulin receptor recycling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolic pathways, organism-specific biosystem (from KEGG)
Metabolic pathways, organism-specific biosystem
Metabolic pathways
Oxidative phosphorylation, organism-specific biosystem (from KEGG)
Oxidative phosphorylation, organism-specific biosystem
Oxidative phosphorylation
Oxidative phosphorylation, conserved biosystem (from KEGG)
Oxidative phosphorylation, conserved biosystem
Oxidative phosphorylation
Phagosome, organism-specific biosystem (from KEGG)
Phagosome, organism-specific biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
Phagosome, conserved biosystem (from KEGG)
Phagosome, conserved biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by Insulin receptor, organism-specific biosystem (from REACTOME)
Signaling by Insulin receptor, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
proton-transporting ATPase activity, rotational mechanism	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
ATP hydrolysis coupled proton transport	IEA Inferred from Electronic Annotation more info
ion transport	IEA Inferred from Electronic Annotation more info
proton transport	IEA Inferred from Electronic Annotation more info
transport	IEA Inferred from Electronic Annotation more info
vacuolar acidification	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
fungal-type vacuole membrane	TAS Traceable Author Statement more info	PubMed
integral to membrane	ISM Inferred from Sequence Model more info	PubMed
membrane	IEA Inferred from Electronic Annotation more info
proton-transporting V-type ATPase, V1 domain	IEA Inferred from Electronic Annotation more info
vacuolar membrane	IEA Inferred from Electronic Annotation more info
vacuolar proton-transporting V-type ATPase, V1 domain	TAS Traceable Author Statement more info	PubMed
vacuole	IEA Inferred from Electronic Annotation more info

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General protein information

Names: Vma5p

NP_012843.1

EC 3.6.3.14

Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9

Range

285030..286208

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179646.1 → NP_012843.1 Vma5p [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P31412

Conserved Domains (1) summary

cl15414
Location:6 – 392
Blast Score: 1433

V-ATPase_C; V-ATPase subunit C