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    TRIM5 tripartite motif containing 5 [ Homo sapiens ]

    Gene ID: 85363, updated on 13-May-2012

    Summary

    Official Symbol
    TRIM5provided by HGNC
    Official Full Name
    tripartite motif containing 5provided by HGNC
    Primary source
    HGNC:16276
    See related
    Ensembl:ENSG00000132256; HPRD:07004; MIM:608487; Vega:OTTHUMG00000066893
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    RNF88; TRIM5alpha
    Summary
    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

    Genomic context

    Location :
    11p15
    Sequence :
    Chromosome: 11; NC_000011.9 (5684425..5706339, complement)
    See TRIM5 in Epigenomics, MapViewer

    Chromosome 11 - NC_000011.9Genomic Context describing neighboring genes Neighboring gene TRIM6-TRIM34 readthrough Neighboring gene tripartite motif containing 6 Neighboring gene tripartite motif containing 34 Neighboring gene tripartite motif containing 78, pseudogene Neighboring gene tripartite motif containing 22 Neighboring gene olfactory receptor, family 52, subfamily U, member 1 pseudogene

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    HIV-1 protein interactions

    Protein Gene Interaction Pubs
    capsid gag HIV-1 infection is modestly restricted by human TRIM5alpha, which acts on the incoming HIV-1 capsid PubMed
    gag HIV-1 capsid is more rapidly degraded in cells expressing monkey TRIM5alpha than in cells expressing human TRIM5alpha PubMed
    gag TRIM5alpha associates with HIV-1 capsid-nucleocapsid complexes assembled in vitro, and the TRIM5alpha interaction with these complexes is dependent on its B30.2(SPRY) domains PubMed
    gag Human TRIM5alpha modestly, but specifically, inhibits an HIV-1 strain carrying a mutation (G89V) in the cyclophilin binding loop in capsid PubMed
    gag Removal of a positively charged residue at position 332 of human TRIM5alpha increases its restricting activity by increasing the ability of TRIM5alpha to bind HIV-1 capsid complexes PubMed

    Go to the HIV-1, Human Protein Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    Q9C035 Q9H0C5 BTBD1    HPRD  PubMed  
    Q9C035 Q9BX70 BTBD2    HPRD  PubMed  
    Q9C035 Q9C035 TRIM5    HPRD  PubMed  
    Q9C035 Q9C030 TRIM6    HPRD  PubMed  
    BioGRID:124491 BioGRID:108431 EWSR1    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113852 LZTR1    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:111125 PCBD1    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113188 SUMO1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:124491 BioGRID:124491 TRIM5    BioGRID  PubMed Affinity Capture-Western; Biochemical Activity; Two-hybrid 
    BioGRID:124491 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:124491 BioGRID:113169 UBE2D1    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113170 UBE2D2    BioGRID  PubMed Biochemical Activity; Two-hybrid 
    BioGRID:124491 BioGRID:113171 UBE2D3    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:119641 UBE2D4    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113176 UBE2H    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:109340 UBE2K    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113182 UBE2N    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:127156 UBE2U    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113183 UBE2V1    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:113184 UBE2V2    BioGRID  PubMed Two-hybrid 
    BioGRID:124491 BioGRID:116303 ZWINT    BioGRID  PubMed Affinity Capture-MS 

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    ligase activity IEA
    Inferred from Electronic Annotation
    more info
     
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    interspecies interaction between organisms IEA
    Inferred from Electronic Annotation
    more info
     
    protein trimerization IDA
    Inferred from Direct Assay
    more info
    PubMed 
    response to virus IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    cytoplasmic mRNA processing body IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytoplasmic mRNA processing body IEA
    Inferred from Electronic Annotation
    more info
     
    intracellular IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    tripartite motif-containing protein 5
    Names
    tripartite motif-containing protein 5
    ring finger protein 88
    tripartite motif protein TRIM5
    tripartite motif containing 5 transcript variant iota
    tripartite motif containing 5 transcript variant kappa

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_029122.1 RefSeqGene

      Range
      5001..26915
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_033034.2NP_149023.2  tripartite motif-containing protein 5 isoform alpha

      Status: REVIEWED

      Description
      Transcript Variant: This variant (alpha) encodes the longest isoform (alpha).
      Source sequence(s)
      AC015691, AK027593, CN334837, DA475412
      Consensus CDS
      CCDS31393.1
      UniProtKB/Swiss-Prot
      Q9C035
      Related
      ENSP00000369373, OTTHUMP00000069811, ENST00000380034, OTTHUMT00000143360
      Conserved Domains (5) summary
      cd00021
      Location:93130
      Blast Score: 101
      BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
      cd00162
      Location:1459
      Blast Score: 116
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      pfam01496
      Location:178256
      Blast Score: 88
      V_ATPase_I; V-type ATPase 116kDa subunit family
      pfam13923
      Location:1159
      Blast Score: 111
      zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)
      cl02614
      Location:356491
      Blast Score: 209
      SPRY; SPRY domain
    2. NM_033092.2NP_149083.2  tripartite motif-containing protein 5 isoform gamma

      Status: REVIEWED

      Description
      Transcript Variant: This variant (gamma) includes an alternate segment in the 3' coding region, compared to variant alpha, which results in a frameshift. The resulting protein (isoform gamma) has a shorter and distinct C-terminus, compared to isoform alpha.
      Source sequence(s)
      AC015691, AF220027, AK074363, BC021258, CN334837, DA475412
      Consensus CDS
      CCDS31394.1
      UniProtKB/Swiss-Prot
      Q9C035
      Related
      ENSP00000380058, OTTHUMP00000069813, ENST00000396847, OTTHUMT00000143363
      Conserved Domains (4) summary
      cd00021
      Location:93130
      Blast Score: 97
      BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
      cd00162
      Location:1459
      Blast Score: 116
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      pfam01496
      Location:178256
      Blast Score: 89
      V_ATPase_I; V-type ATPase 116kDa subunit family
      pfam13923
      Location:1159
      Blast Score: 112
      zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)
    3. NM_033093.2NP_149084.2  tripartite motif-containing protein 5 isoform delta

      Status: REVIEWED

      Description
      Transcript Variant: This variant (delta) differs in the 5' UTR and uses an alternate splice site in the 3' terminal exon, which results in a frameshift, compared to variant alpha. The encoded isoform (delta) has a shorter and distinct C-terminus, compared to isoform alpha.
      Source sequence(s)
      AC015691, AC109341, DA475412
      Consensus CDS
      CCDS31392.1
      UniProtKB/Swiss-Prot
      Q9C035
      Related
      ENSP00000380064, ENST00000396855
      Conserved Domains (4) summary
      cd00021
      Location:93130
      Blast Score: 101
      BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
      cd00162
      Location:1459
      Blast Score: 122
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      pfam01496
      Location:178256
      Blast Score: 88
      V_ATPase_I; V-type ATPase 116kDa subunit family
      pfam13923
      Location:1159
      Blast Score: 118
      zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000011.9 Reference GRCh37.p5 Primary Assembly

      Range
      5684425..5706339, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000143.1 Alternate HuRef

      Range
      5344050..5365972, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC015691.6 None
    genomic AC109341.7 (111046..130159) None
    genomic CH471064.2 EAW68771.1
      EAW68772.1
      EAW68773.1
      EAW68774.1
      EAW68775.1
      EAW68776.1
      EAW68777.1
    genomic DQ301444.1 ABC00997.1
    genomic DQ301445.1 ABC00998.1
    genomic DQ301446.1 ABC00999.1
    genomic DQ301447.1 ABC01000.1
    genomic DQ301448.1 ABC01001.1
    genomic DQ301449.1 ABC01002.1
    genomic DQ301450.1 ABC01003.1
    genomic DQ301451.1 ABC01004.1
    genomic DQ301452.1 ABC01005.1
    genomic DQ301453.1 ABC01006.1
    genomic DQ301454.1 ABC01007.1
    genomic DQ301455.1 ABC01008.1
    genomic DQ301456.1 ABC01009.1
    genomic DQ301457.1 ABC01010.1
    genomic DQ301458.1 ABC01011.1
    genomic DQ301459.1 ABC01012.1
    genomic DQ301460.1 ABC01013.1
    genomic DQ301461.1 ABC01014.1
    genomic DQ301462.1 ABC01015.1
    genomic DQ301463.1 ABC01016.1
    genomic DQ301464.1 ABC01017.1
    genomic DQ301465.1 ABC01018.1
    genomic DQ301466.1 ABC01019.1
    genomic DQ301467.1 ABC01020.1
    genomic DQ301468.1 ABC01021.1
    genomic DQ301469.1 ABC01022.1
    genomic DQ301470.1 ABC01023.1
    genomic DQ301471.1 ABC01024.1
    genomic DQ301472.1 ABC01025.1
    genomic DQ301473.1 ABC01026.1
    genomic DQ301474.1 ABC01027.1
    genomic DQ301475.1 ABC01028.1
    genomic DQ301476.1 ABC01029.1
    genomic DQ301477.1 ABC01030.1
    genomic DQ301478.1 ABC01031.1
    genomic DQ301479.1 ABC01032.1
    genomic DQ301480.1 ABC01033.1
    genomic EU260465.1 ABW96352.1
    mRNA AB362928.1 BAG16811.1
    mRNA AF220025.1 AAG53479.1
    mRNA AF220026.1 AAG53480.1
    mRNA AF220027.1 AAG53481.1
    mRNA AF220028.1 AAG53482.1
    mRNA AF220029.1 AAG53483.1
    mRNA AK027593.1 BAB55218.1
    mRNA AK074363.1 None
    mRNA AY625000.1 AAT48101.1
    mRNA BC020770.1 AAH20770.1
    mRNA BC021258.2 AAH21258.1
    mRNA BX647152.1 None
    mRNA CN334837.1 None
    mRNA DA475412.1 None
    mRNA DQ288685.1 ABB90543.1
    mRNA JF928461.1 AEN14475.1
    mRNA JF928462.1 AEN14476.1
    other-genetic HQ447250.1 ADQ31737.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q05CU3 GenPept UniProtKB/TrEMBL:Q05CU3
    Q9C035.1 GenPept UniProtKB/Swiss-Prot:Q9C035

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