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    MAD3 Mad3p [ Saccharomyces cerevisiae S288c ]

    Gene ID: 853439, updated on 21-Jan-2012
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    Summary

    Gene symbol
    MAD3
    Gene description
    Mad3p
    Primary source
    SGD:S000003550
    Locus tag
    YJL013C
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Saccharomyces cerevisiae S288c (strain: S288c)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
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    Genomic context

    Sequence :
    Chromosome: X; NC_001142.9 (409493..411040, complement)
    See MAD3 in MapViewer

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene hypothetical protein Neighboring gene Cct3p Neighboring gene Vtc4p Neighboring gene Rpc17p

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Identification of Saccharomyces cerevisiae spindle pole body remodeling factors. Greenland KB, et al. PLoS One, 2010 Nov 12. PMID 21103054.
    2. Epistatic relationships reveal the functional organization of yeast transcription factors. Zheng J, et al. Mol Syst Biol, 2010 Oct 5. PMID 20959818.
    3. The genetic landscape of a cell. Costanzo M, et al. Science, 2010 Jan 22. PMID 20093466.
    4. The synaptonemal complex protein, Zip1, promotes the segregation of nonexchange chromosomes at meiosis I. Newnham L, et al. Proc Natl Acad Sci U S A, 2010 Jan 12. PMID 20080752.
    5. Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast. Chiroli E, et al. Mol Biol Cell, 2009 May. PMID 19339280.

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation
      Title: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.
    2. Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
      Title: Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
    3. model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction
      Title: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.
    4. In vivo Mad3 phosphorylation depends on the Polo kinase Cdc5 and, to a minor extent, the Aurora B kinase Ipl1.
      Title: Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast.
    Submit: New GeneRIF Correction
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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    BioGRID:33742 BioGRID:34022 ABF1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35843 AIM38    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32891 AIM4    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35658 APC1    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:32183 ARO1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:34861 ASG1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36749 BIM1    BioGRID  PubMed Negative Genetic; Synthetic Lethality 
    BioGRID:33742 BioGRID:33440 BUB1    BioGRID  PubMed Negative Genetic; Synthetic Rescue 
    BioGRID:33742 BioGRID:35231 BUB2    BioGRID  PubMed Phenotypic Enhancement 
    BioGRID:33742 BioGRID:34429 BUB3    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:33742 BioGRID:33135 CDC20    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western; Co-fractionation; Phenotypic Enhancement; Two-hybrid 
    BioGRID:33742 BioGRID:32859 CDC28    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:32038 CDC7    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36169 CHL1    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:35376 CIK1    BioGRID  PubMed Negative Genetic; Synthetic Lethality 
    BioGRID:33742 BioGRID:34734 CIN1    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:35922 CIN2    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:36668 CIN8    BioGRID  PubMed Negative Genetic; Phenotypic Suppression; Synthetic Lethality 
    BioGRID:33742 BioGRID:31725 CLN3    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:35835 CSE2    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:35254 CTF18    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect; Synthetic Lethality 
    BioGRID:33742 BioGRID:36159 CTF19    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect; Synthetic Lethality 
    BioGRID:33742 BioGRID:36302 CTF4    BioGRID  PubMed Synthetic Growth Defect 
    BioGRID:33742 BioGRID:36625 CTF8    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:32985 CTP1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:33360 DAM1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:30968 DCC1    BioGRID  PubMed Synthetic Growth Defect 
    BioGRID:33742 BioGRID:31961 DUN1    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:33186 DUO1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32415 EAF1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31747 FUN30    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35676 GIM3    BioGRID  PubMed Negative Genetic; Synthetic Lethality 
    BioGRID:33742 BioGRID:36728 GIM4    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:35049 GIM5    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:31041 HCM1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35909 HFI1    BioGRID  PubMed Positive Genetic 
    BioGRID:33742 BioGRID:32710 HHF1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:33848 IME1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35976 IPL1    BioGRID  PubMed Biochemical Activity 
    BioGRID:33742 BioGRID:32389 IRC3    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:33875 JHD2    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36310 KAR3    BioGRID  PubMed Negative Genetic; Synthetic Lethality 
    BioGRID:33742 BioGRID:32636 KIP1    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:33729 MAD2    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:33742 BioGRID:34687 MBF1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35532 MCK1    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:32374 MCM21    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:32836 MEC1    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:34044 MIF2    BioGRID  PubMed Synthetic Growth Defect 
    BioGRID:33742 BioGRID:31584 MMS22    BioGRID  PubMed Positive Genetic 
    BioGRID:33742 BioGRID:33175 MPS2    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:35402 MRE11    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:33257 MSB2    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:31467 NOP56    BioGRID  PubMed Dosage Lethality 
    BioGRID:33742 BioGRID:35929 NSL1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:33320 PAC10    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:36737 PAC2    BioGRID  PubMed Synthetic Growth Defect 
    BioGRID:33742 BioGRID:33582 PFD1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35105 PIF1    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:35725 RAS2    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31793 RFA1    BioGRID  PubMed Phenotypic Suppression 
    BioGRID:33742 BioGRID:35329 RIM13    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:36153 RMI1    BioGRID  PubMed Positive Genetic 
    BioGRID:33742 BioGRID:35511 RPD3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32210 SAC3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:33010 SAP4    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:30987 SAT4    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:31922 SCM3    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:32634 SEF1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36133 SGF11    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31352 SIC1    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:35603 SIN4    BioGRID  PubMed Two-hybrid 
    BioGRID:33742 BioGRID:31186 SMC2    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:32412 SPC110    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:35293 SPC24    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32454 SPT3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36137 SSN3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32663 STU1    BioGRID  PubMed Phenotypic Suppression; Synthetic Lethality 
    BioGRID:33742 BioGRID:31320 STU2    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:35045 TAF13    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31981 THI3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31109 TUB2    BioGRID  PubMed Synthetic Lethality 
    BioGRID:33742 BioGRID:35081 TUB3    BioGRID  PubMed Synthetic Growth Defect; Synthetic Lethality 
    BioGRID:33742 BioGRID:31051 YCR076C    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31469 YKE2    BioGRID  PubMed Negative Genetic; Synthetic Growth Defect 
    BioGRID:33742 BioGRID:32730 YPK3    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:36138 YPL041C    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:31529 YPT6    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:33698 ZAP1    BioGRID  PubMed Negative Genetic 
    BioGRID:33742 BioGRID:32338 ZIP1    BioGRID  PubMed Phenotypic Enhancement 
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    General gene information

    Pathways from BioSystems

    • Cell cycle - yeast, organism-specific biosystem (from KEGG)
      Cell cycle - yeast, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
    • Cell cycle - yeast, conserved biosystem (from KEGG)
      Cell cycle - yeast, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...

    Gene Ontology Provided by GO

    Function Evidence Code Pubs
    molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    cell cycle checkpoint IEA
    Inferred from Electronic Annotation
    more info
    		  
    cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    distributive segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    mitosis IEA
    Inferred from Electronic Annotation
    more info
    		  
    mitotic cell cycle G2/M transition decatenation checkpoint IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    mitotic cell cycle spindle assembly checkpoint IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    mitotic cell cycle spindle assembly checkpoint IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    mitotic checkpoint complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    mitotic checkpoint complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    mitotic checkpoint complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Names
    Mad3p
    NP_012521.1
    • Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover
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    NCBI Reference Sequences (RefSeq)

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9

      Range
      409493..411040, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181447.1 → NP_012521.1  Mad3p [Saccharomyces cerevisiae S288c]

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P47074
      Conserved Domains (2) summary
      cl07066
      Location:58 – 189
      Blast Score: 432
      Mad3_BUB1_I; Mad3/BUB1 homology region 1
      pfam08171
      Location:330 – 400
      Blast Score: 245
      Mad3_BUB1_II; Mad3/BUB1 homology region 2
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic BK006943.2 DAA08780.1
    Protein Accession Links
    GenePept Link UniProtKB Link
    P47074.1 GenPept
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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