TFG1 Tfg1p [Saccharomyces cerevisiae S288c] - Gene

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Summary

Gene symbol: TFG1
Gene description: Tfg1p
Primary source: SGD:S000003418
Locus tag: YGR186W
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Saccharomyces cerevisiae S288c (strain: S288c)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RAP74; SSU71

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Genomic context

Sequence :: Chromosome: VII; NC_001139.9 (867774..869981)

See TFG1 in MapViewer

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TFIIF stimulates formation of the first two phosphodiester bonds and dramatically stabilizes a short RNA-DNA hybrid in the RNA polymerase II (RNAPII) active center.
Title: Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization.
Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of RNA polymerase II (TFIIB) near the active center of RNAP II where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection.
Title: Evidence that the Tfg1/Tfg2 dimer interface of TFIIF lies near the active center of the RNA polymerase II initiation complex.

Submit: New GeneRIF Correction

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
NP_011702.1	NC_001147.4	ADH1	BIND	PubMed	Tfg1 interacts with ADH1
NP_011702.1	NP_014004.1	FCP1	BIND	PubMed	Tfg1 interacts with Fcp1.
NP_011702.1	NP_015411.1	SUA7	BIND	PubMed	TFIIB interacts with Tfg1 within the preinitiation complex
BioGRID:33438	BioGRID:32891	AIM4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:31358	ARP6	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:34230	BAS1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:31123	BUD27	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:35549	CAF40	BioGRID	PubMed	Positive Genetic
BioGRID:33438	BioGRID:32859	CDC28	BioGRID	PubMed	Biochemical Activity
BioGRID:33438	BioGRID:35070	COG8	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33801	CYC1	BioGRID	PubMed	Synthetic Rescue
BioGRID:33438	BioGRID:33952	DOA1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33205	DST1	BioGRID	PubMed	Affinity Capture-MS; Synthetic Lethality
BioGRID:33438	BioGRID:35161	ERG6	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33773	ESS1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:35456	FCP1	BioGRID	PubMed	Co-crystal Structure; Reconstituted Complex
BioGRID:33438	BioGRID:35635	GCR2	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:35584	GIS2	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:33438	BioGRID:32691	HIR1	BioGRID	PubMed	Positive Genetic
BioGRID:33438	BioGRID:34392	HTZ1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33086	HUR1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32940	ISW1	BioGRID	PubMed	Positive Genetic
BioGRID:33438	BioGRID:31947	IWR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:34863	MET18	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:35541	MON2	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:35402	MRE11	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33129	NAB2	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:33438	BioGRID:35703	NAF1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:31306	RAD5	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:34605	RPB10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:34399	RPB11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:34547	RPB2	BioGRID	PubMed	Affinity Capture-MS; Biochemical Activity
BioGRID:33438	BioGRID:34967	RPB3	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:33438	BioGRID:33617	RPB4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:32853	RPB5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:32464	RPB7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:34618	RPB8	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:33178	RPB9	BioGRID	PubMed	Affinity Capture-MS; Synthetic Growth Defect
BioGRID:33438	BioGRID:31152	RPN11	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:31921	RPO21	BioGRID	PubMed	Affinity Capture-MS; Biochemical Activity; Co-purification; Synthetic Growth Defect; Synthetic Rescue
BioGRID:33438	BioGRID:36359	RPO26	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:36869	RSP5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:32210	SAC3	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32420	SEM1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33591	SET2	BioGRID	PubMed	Synthetic Growth Defect
BioGRID:33438	BioGRID:33181	SGF73	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32807	SIF2	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:34400	SIN3	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32561	SMT3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:33124	SOH1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:36698	SPF1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32454	SPT3	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33308	SPT4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:35159	SPT5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:31330	SPT8	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:36756	SRB4	BioGRID	PubMed	Affinity Capture-Western; Co-purification
BioGRID:33438	BioGRID:30946	SRO9	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:36257	SUA7	BioGRID	PubMed	Synthetic Rescue
BioGRID:33438	BioGRID:33585	SWI3	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32391	SWR1	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:33524	TAF1	BioGRID	PubMed	Co-localization
BioGRID:33438	BioGRID:36052	TAF14	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification; Reconstituted Complex; Two-hybrid
BioGRID:33438	BioGRID:36002	TCO89	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:34103	TFA1	BioGRID	PubMed	Co-purification
BioGRID:33438	BioGRID:34193	TFA2	BioGRID	PubMed	Co-purification
BioGRID:33438	BioGRID:36230	TFB4	BioGRID	PubMed	Co-purification
BioGRID:33438	BioGRID:33438	TFG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:33249	TFG2	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Co-purification; Synthetic Growth Defect
BioGRID:33438	BioGRID:33595	TPK1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:35981	TPK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:36901	UBP3	BioGRID	PubMed	Co-purification; Negative Genetic
BioGRID:33438	BioGRID:31160	UBP6	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:36319	URN1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:33438	BioGRID:32251	VPS64	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:32537	VPS72	BioGRID	PubMed	Negative Genetic
BioGRID:33438	BioGRID:31529	YPT6	BioGRID	PubMed	Negative Genetic

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General gene information

Homology

Homologs of the TFG1 gene: The TFG1 gene is conserved in S.cerevisiae, K.lactis, and E.gossypii.

Pathways from BioSystems

Basal transcription factors, organism-specific biosystem (from KEGG)
Basal transcription factors, organism-specific biosystem
Basal transcription factors
Basal transcription factors, conserved biosystem (from KEGG)
Basal transcription factors, conserved biosystem
Basal transcription factors
Formation of the Early Elongation Complex, organism-specific biosystem (from REACTOME)
Formation of the Early Elongation Complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Gene Expression, organism-specific biosystem (from REACTOME)
Gene Expression, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem (from REACTOME)
Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Pol II CTD phosphorylation and interaction with CE, organism-specific biosystem (from REACTOME)
RNA Pol II CTD phosphorylation and interaction with CE, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Pre-transcription Events, organism-specific biosystem (from REACTOME)
RNA Polymerase II Pre-transcription Events, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Promoter Escape, organism-specific biosystem (from REACTOME)
RNA Polymerase II Promoter Escape, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Elongation, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystemcomputationally inferred pathway (not manually curated)
mRNA Capping, organism-specific biosystem (from REACTOME)
mRNA Capping, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
catalytic activity	IEA Inferred from Electronic Annotation more info
core RNA polymerase II binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to core RNA polymerase II recruiting transcription factor activity	IC Inferred by Curator more info	PubMed
protein phosphatase activator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
RNA polymerase II transcriptional preinitiation complex assembly	IC Inferred by Curator more info	PubMed
dephosphorylation of RNA polymerase II C-terminal domain	IDA Inferred from Direct Assay more info	PubMed
dephosphorylation of RNA polymerase II C-terminal domain	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
promoter clearance from RNA polymerase II promoter	IC Inferred by Curator more info	PubMed
regulation of protein serine/threonine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
transcription elongation from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
transcription elongation from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
transcription initiation from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
transcription initiation from RNA polymerase II promoter	IEA Inferred from Electronic Annotation more info
transcription initiation from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
transcriptional start site selection at RNA polymerase II promoter	IGI Inferred from Genetic Interaction more info	PubMed
transcriptional start site selection at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
nucleus	IEA Inferred from Electronic Annotation more info
transcription factor TFIIF complex	IDA Inferred from Direct Assay more info	PubMed

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General protein information

Names: Tfg1p

NP_011702.1

EC 2.7.11.1

TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9

Range

867774..869981

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181315.1 → NP_011702.1 Tfg1p [Saccharomyces cerevisiae S288c]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P41895

Conserved Domains (1) summary

cl15395
Location:295 – 416
Blast Score: 99

TFIIFa; Transcription initiation factor IIF, alpha subunit, N-terminal region of RAP74. Subunit of transcription initiation complex involved in initiation, elongation and promoter escape.Tetramer of 2 alpha and 2 beta TFIIF subunits interacts directly with RNA ...