PEX14 Pex14p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PEX14
Gene description: Pex14p
Primary source: SGD:S000003121
Locus tag: YGL153W
See related: AllianceGenome:SGD:S000003121
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein-macromolecule adaptor activity. Involved in protein import into peroxisome matrix, docking. Located in peroxisomal membrane. Part of peroxisomal importomer complex. Human ortholog(s) of this gene implicated in peroxisome biogenesis disorder 13A. Orthologous to human PEX14 (peroxisomal biogenesis factor 14). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PEX14 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (216273..217298)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using a combination of microscopy and biochemical approaches, we show that a subset of the PMPs, including the receptor docking protein Pex14, localizes to membrane vesicles in S. cerevisiae pex3 cells.
Title: Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins.
Both, Pex14p and Pex17p interact directly with Dyn2p, but in vivo, Pex17p turned out to be prerequisite for an association of Dyn2p with Pex14p.
Title: Pex17p-dependent assembly of Pex14p/Dyn2p-subcomplexes of the peroxisomal protein import machinery.
The membrane-associated import receptor Pex5p together with its docking partner Pex14p forms a gated ion-conducting channel which can be opened to a diameter of about 9 nm by the cytosolic receptor-cargo complex.
Title: The peroxisomal importomer constitutes a large and highly dynamic pore.
Pex14p is required in only catalytic amounts to allow initiation of the selective peroxisome degradation process.
Title: Pex14p is not required for N-starvation induced microautophagy and in catalytic amounts for macropexophagy in Hansenula polymorpha.
Pex14p contains two independent Pex5p binding sites, one in the N- and one in the C-terminus.
Title: Saccharomyces cerevisiae Pex14p contains two independent Pex5p binding sites, which are both essential for PTS1 protein import.
the C terminus of Pex14p contains distinct Pex5p and one Pex7p binding sites and the N terminus could be involved in a Pex5p-Pex14p association inside the peroxisomal membrane
Title: Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites.
Pex3p amino-terminal domain targets to a subdomain of the endoplasmic reticulum and initiates the formation of a preperoxisomal compartment for de novo peroxisome synthesis; peroxisome formation depends on the activity of the genes PEX14 and PEX19
Title: Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the endoplasmic reticulum in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in protein import into peroxisome matrix, docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into peroxisome matrix, docking	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into peroxisome matrix, docking	IEA Inferred from Electronic Annotation more info
involved_in protein import into peroxisome matrix, docking	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into peroxisome matrix, docking	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of peroxisomal importomer complex	IBA Inferred from Biological aspect of Ancestor more info
part_of peroxisomal importomer complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in peroxisomal membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in peroxisomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal membrane	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pex14p

NP_011362.3

Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

216273..217298

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181018.3 → NP_011362.3 TPA: Pex14p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011362.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTZ8, P53112

UniProtKB/TrEMBL

A0A6A5PVD9, A6ZU32, B3LHK6, C7GPQ3, C8Z898, G2WDV2, N1PA10

Conserved Domains (2) summary

TIGR01612
Location:126 → 247

235kDa-fam; reticulocyte binding/rhoptry protein

pfam04695
Location:8 → 53

Pex14_N; Peroxisomal membrane anchor protein (Pex14p) conserved region