DGKE diacylglycerol kinase, epsilon 64kDa [Homo sapiens] - Gene

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Summary

Official Symbol: DGKEprovided by HGNC
Official Full Name: diacylglycerol kinase, epsilon 64kDaprovided by HGNC
Primary source: HGNC:2852
See related: Ensembl:ENSG00000153933; HPRD:03259; MIM:601440
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DGK; DAGK5; DAGK6
Summary: Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 17q22

Sequence :: Chromosome: 17; NC_000017.10 (54911460..54946036)

See DGKE in Epigenomics, MapViewer

Chromosome 17 - NC_000017.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

diacylglycerol kinase-epsilon (DGKepsilon) has less preference for the acyl chain at the sn-1 position of diacylglycerol (DAG) than the one at the sn-2 position
Title: Substrate specificity of diacylglycerol kinase-epsilon and the phosphatidylinositol cycle.
DGK activity is reduced by oxidative stress in human mesangial cells cultured under high glucose conditions.
Title: Reversal of redox-dependent inhibition of diacylglycerol kinase by antioxidants in mesangial cells exposed to high glucose.
Data show that Diacylglycerol that 2-arachidonoyl glycerol is a very poor substrate for either the epsilon or the zeta isoforms of diacylglycerol kinases.
Title: Endocannabinoids and diacylglycerol kinase activity.
Substrate specificity of diacylglycerol kinase epsilon is determined by selectivity of the sn-1 and sn-2 acyl chains phosphatidic acid or diacylglycerol.
Title: Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol.
Observational study of gene-disease association. (HuGE Navigator)
Title: The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature.
The alpha and zeta isoforms of diacylglycerol kinase are inhibited by 2,3-dioleoylglycerol, but not the more substrate-selective epsilon isoform
Title: Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase.
The hydrophobic domain of diacylglycerol kinase epsilon does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane.
Title: Role of the hydrophobic segment of diacylglycerol kinase epsilon.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Many sequence variants affecting diversity of adult human height.

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Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:114096	BioGRID:115950	NUDC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114096	BioGRID:123673	NUF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114096	BioGRID:115852	PAICS	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114096	BioGRID:111186	PDHA1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114096	BioGRID:112316	SET	BioGRID	PubMed	Affinity Capture-MS
BioGRID:114096	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway	IEA Inferred from Electronic Annotation more info
blood coagulation	TAS Traceable Author Statement more info
intracellular signal transduction	IEA Inferred from Electronic Annotation more info
phospholipid biosynthetic process	TAS Traceable Author Statement more info	PubMed
platelet activation	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
integral to membrane	IEA Inferred from Electronic Annotation more info
plasma membrane	TAS Traceable Author Statement more info

General protein information

Preferred Names: diacylglycerol kinase epsilon

Names: diacylglycerol kinase epsilon; DGK-epsilon; DAG kinase epsilon; diglyceride kinase epsilon

NP_003638.1

EC 2.7.1.107

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003647.2 → NP_003638.1 diacylglycerol kinase epsilon

Status: REVIEWED

Source sequence(s)

AC015912, AL555194, BC130629, BM682264, DB221284

Consensus CDS

CCDS11590.1

UniProtKB/TrEMBL

A1L4Q0

UniProtKB/Swiss-Prot

P52429

Related

ENSP00000284061, ENST00000284061

Conserved Domains (4) summary

cd00029
Location:125 – 174
Blast Score: 113

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl00040
Location:60 – 109
Blast Score: 116

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl01255
Location:221 – 349
Blast Score: 389

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:369 – 524
Blast Score: 610

DAGK_acc; Diacylglycerol kinase accessory domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000017.10 Reference GRCh37.p5 Primary Assembly

Range

54911460..54946036

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000149.1 Alternate HuRef

Range

50271649..50306289

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC015912.16 (26157..55574)	None
genomic	AC106858.12	None
genomic	AF136745.1	AAD45666.1
genomic	CH471109.2	EAW94526.1
mRNA	AB209365.1	BAD92602.1
mRNA	AK057591.1	None
mRNA	AK129803.1	None
mRNA	AK293039.1	BAF85728.1
mRNA	AK310668.1	None
mRNA	AL555194.3	None
mRNA	BC022297.1	AAH22297.1
mRNA	BC130629.1	AAI30630.1
mRNA	BC130631.1	AAI30632.1
mRNA	BM682264.1	None
mRNA	DB221284.1	None
mRNA	U49379.1	AAC50497.1
other-genetic	HQ258315.1	ADR83069.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P52429.1	GenPept	UniProtKB/Swiss-Prot:P52429
Q59FU4	GenPept	UniProtKB/TrEMBL:Q59FU4
Q8TBM4	GenPept	UniProtKB/TrEMBL:Q8TBM4