DGKZ diacylglycerol kinase, zeta [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: DGKZprovided by HGNC
Official Full Name: diacylglycerol kinase, zetaprovided by HGNC
Primary source: HGNC:2857
See related: Ensembl:ENSG00000149091; HPRD:03260; MIM:601441; Vega:OTTHUMG00000166437
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DAGK5; DAGK6; DGK-ZETA; hDGKzeta
Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]

Help top of page

Genomic context

Location :: 11p11.2

Sequence :: Chromosome: 11; NC_000011.9 (46354455..46402104)

See DGKZ in Epigenomics, MapViewer

Chromosome 11 - NC_000011.9 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGK-zeta translocated rapidly to the plasma membrane at early stages of immunological synapse (IS) formation independent of enzyme activity; study highlights a DGKzeta-specific function for local diacylglycerol metabolism at the IS and offers new clues...
Title: Diacylglycerol kinase ζ controls diacylglycerol metabolism at the immunological synapse.
Nucleosome assembly protein (NAP) 1-like 1 (NAP1L1) and NAP1-like 4 (NAP1L4) are identified as novel DGKzeta binding partners.
Title: Interaction of nucleosome assembly proteins abolishes nuclear localization of DGKζ by attenuating its association with importins.
Data show that Diacylglycerol that 2-arachidonoyl glycerol is a very poor substrate for either the epsilon or the zeta isoforms of diacylglycerol kinases.
Title: Endocannabinoids and diacylglycerol kinase activity.
None of SNPs of diacylglycerol kinase zeta tested showed association with bipolar disorder in Sardinian sample.
Title: The diacylglycerol kinase eta gene and bipolar disorder: a replication study in a Sardinian sample.
In DGKzeta-deficient fibroblasts PAK1 phosphorylation and Rac1-RhoGDI dissociation were attenuated, leading to reduced Rac1 activation after platelet-derived growth factor stimulation.
Title: Diacylglycerol kinase zeta regulates actin cytoskeleton reorganization through dissociation of Rac1 from RhoGDI.
PKD activation is induced by DGKzeta, suggesting DGK is an upstream regulator of oxidative stress-induced activation of the PKD signaling pathway in intestinal epithelial cells.
Title: Diacylglycerol kinase regulation of protein kinase D during oxidative stress-induced intestinal cell injury.
2,3-dioleoylglycerol binds to a site on the alpha and zeta isoforms of diacylglycerol kinase that is exposed as a consequence of the substrate binding to the active site.
Title: Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase.
structural domain requirements for translocation and activity
Title: Dynamics of diacylglycerol kinase zeta translocation in living T-cells. Study of the structural domain requirements for translocation and activity.
DGKzeta-derived phosphatidic acid acts as a mediator of mTOR signaling
Title: Modulation of the mammalian target of rapamycin pathway by diacylglycerol kinase-produced phosphatidic acid.
PKC alpha phosphorylates diacylglycerol kinase zeta in cells, and this phosphorylation inhibits its kinase activity to remove cellular diacylglycerol, thereby affecting cell growth.
Title: Protein kinase C alpha phosphorylates and negatively regulates diacylglycerol kinase zeta.

Submit: New GeneRIF Correction See all (14)

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Association of IFIH1 and other autoimmunity risk alleles with selective IgA deficiency.

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
Q13574	P11532	DMD	HPRD	PubMed
Q13574	P01112	HRAS	HPRD	PubMed
Q13574	P48357	LEPR	HPRD	PubMed
Q13574	P19174	PLCG1	HPRD	PubMed
Q13574	GRINL1A downstream protein Gdown1	POLR2M	HPRD	PubMed
Q13574	P17252	PRKCA	HPRD	PubMed
Q13574	RAS guanyl nucleotide releasing protein 1	RASGRP1	HPRD	PubMed
Q13574	P06400	RB1	HPRD	PubMed
Q13574	P28749	RBL1	HPRD	PubMed
Q13574	Q08999	RBL2	HPRD	PubMed
Q13574	P21673	SAT1	HPRD	PubMed
Q13574	Q13424	SNTA1	HPRD	PubMed
Q13574	Q13884	SNTB1	HPRD	PubMed
Q13574	Q13425	SNTB2	HPRD	PubMed
Q13574	Q9NSN8	SNTG1	HPRD	PubMed
Q13574	Q96L92	SNX27	HPRD	PubMed
Q13574	P12931	SRC	HPRD	PubMed
BioGRID:114095	BioGRID:199245	Dmd	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114095	BioGRID:109501	HRAS	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114095	BioGRID:111583	MAPK6	BioGRID	PubMed	Two-hybrid
BioGRID:114095	BioGRID:119157	PARD6A	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114095	BioGRID:111308	PIK3CA	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:114095	BioGRID:111351	PLCG1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114095	BioGRID:123498	POLR2M	BioGRID	PubMed	Two-hybrid
BioGRID:114095	BioGRID:115429	RASGRP1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:114095	BioGRID:112210	SAT1	BioGRID	PubMed	Two-hybrid
BioGRID:114095	BioGRID:112523	SNTA1	BioGRID	PubMed	Reconstituted Complex
BioGRID:114095	BioGRID:112524	SNTB1	BioGRID	PubMed	Reconstituted Complex
BioGRID:114095	BioGRID:112528	SNTB2	BioGRID	PubMed	Reconstituted Complex
BioGRID:114095	BioGRID:113269	VHL	BioGRID	PubMed	Affinity Capture-Western; Co-localization

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enzyme inhibitor activity	IEA Inferred from Electronic Annotation more info
lipid kinase activity	IDA Inferred from Direct Assay more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein C-terminus binding	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway	IEA Inferred from Electronic Annotation more info
blood coagulation	TAS Traceable Author Statement more info
cell migration	NAS Non-traceable Author Statement more info	PubMed
intracellular signal transduction	IEA Inferred from Electronic Annotation more info
lipid phosphorylation	IDA Inferred from Direct Assay more info	PubMed
mitotic cell cycle G1/S transition DNA damage checkpoint	IGI Inferred from Genetic Interaction more info	PubMed
negative regulation of Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
negative regulation of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
platelet activation	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
lamellipodium	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
plasma membrane	TAS Traceable Author Statement more info

General protein information

Preferred Names: diacylglycerol kinase zeta

Names: diacylglycerol kinase zeta; DAG kinase zeta; diglyceride kinase zeta; diacylglycerol kinase, zeta 104kDa

NP_001099010.1

EC 2.7.1.107

NP_001186195.1

EC 2.7.1.107

NP_001186196.1

EC 2.7.1.107

NP_001186197.1

EC 2.7.1.107

NP_003637.2

EC 2.7.1.107

NP_963290.1

EC 2.7.1.107

NP_963291.2

EC 2.7.1.107

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105540.1 → NP_001099010.1 diacylglycerol kinase zeta isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) encodes the longest isoform (4).

Source sequence(s)

AC116021, U94905

Consensus CDS

CCDS41640.1

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000412178, OTTHUMP00000232891, ENST00000454345, OTTHUMT00000389772

Conserved Domains (6) summary

cd00204
Location:983 – 1099
Blast Score: 217

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:482 – 805
Blast Score: 194

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:361 – 420
Blast Score: 109

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:983 – 1077
Blast Score: 180

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:486 – 607
Blast Score: 450

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:635 – 792
Blast Score: 628

DAGK_acc; Diacylglycerol kinase accessory domain
NM_001199266.1 → NP_001186195.1 diacylglycerol kinase zeta isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) has an alternate 5' sequence including the coding region and extra nts CAG and an extra segment at alternate splice sites, respectively, in the CDS, compared to variant 4. The resulting isoform (5) has a shorter and distinct N-terminus, and an internal additional aa and an additional segment, compared to isoform 4.

Source sequence(s)

AK225774, CB959911, U51477

Consensus CDS

CCDS55757.1

UniProtKB/TrEMBL

E9PPW4

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000436291, OTTHUMP00000233029, ENST00000527911, OTTHUMT00000389999

Conserved Domains (6) summary

cd00204
Location:795 – 916
Blast Score: 201

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:294 – 617
Blast Score: 194

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:173 – 232
Blast Score: 107

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:795 – 894
Blast Score: 166

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:298 – 419
Blast Score: 452

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:447 – 604
Blast Score: 626

DAGK_acc; Diacylglycerol kinase accessory domain
NM_001199267.1 → NP_001186196.1 diacylglycerol kinase zeta isoform 6

Status: REVIEWED

Description

Transcript Variant: This variant (6) has an alternate 5' sequence including the coding region compared to variant 4. The resulting isoform (6) has a shorter and distinct N-terminus, compared to isoform 4.

Source sequence(s)

CB959911, U51477

Consensus CDS

CCDS55759.1

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000395684, OTTHUMP00000238021, ENST00000456247, OTTHUMT00000398328

Conserved Domains (6) summary

cd00204
Location:794 – 910
Blast Score: 215

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:293 – 616
Blast Score: 193

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:172 – 231
Blast Score: 108

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:794 – 888
Blast Score: 179

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:297 – 418
Blast Score: 452

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:446 – 603
Blast Score: 625

DAGK_acc; Diacylglycerol kinase accessory domain
NM_001199268.1 → NP_001186197.1 diacylglycerol kinase zeta isoform 7

Status: VALIDATED

Description

Transcript Variant: This variant (7) has an alternate 5' sequence including the coding region and extra nts CAG at an alternate splice site in the CDS, and lacks an in-frame coding exon, compared to variant 4. The resulting isoform (7) has a shorter and distinct N-terminus and an internal additional aa, and lacks an internal segment, compared to isoform 4.

Source sequence(s)

AK294888, CB959911, U51477

Consensus CDS

CCDS55758.1

UniProtKB/TrEMBL

B7Z2M9

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000320340, ENST00000318201

Conserved Domains (5) summary

cd00204
Location:772 – 888
Blast Score: 216

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:271 – 594
Blast Score: 192

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam12796
Location:772 – 866
Blast Score: 179

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:275 – 396
Blast Score: 452

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:424 – 581
Blast Score: 627

DAGK_acc; Diacylglycerol kinase accessory domain
NM_003646.3 → NP_003637.2 diacylglycerol kinase zeta isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an alternate 5' sequence including the coding region and extra nts CAG at an alternate splice site in the CDS, compared to variant 4. The resulting isoform (2) has a shorter and distinct N-terminus and an internal additional aa, compared to isoform 4.

Source sequence(s)

BU849628, CB959911, U94905

Consensus CDS

CCDS44580.1

UniProtKB/TrEMBL

G3V0F6

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000391021, OTTHUMP00000238022, ENST00000421244, OTTHUMT00000398329

Conserved Domains (6) summary

cd00204
Location:795 – 911
Blast Score: 215

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:294 – 617
Blast Score: 194

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:173 – 232
Blast Score: 108

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:795 – 889
Blast Score: 179

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:298 – 419
Blast Score: 452

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:447 – 604
Blast Score: 625

DAGK_acc; Diacylglycerol kinase accessory domain
NM_201532.2 → NP_963290.1 diacylglycerol kinase zeta isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) has an alternate 5' sequence including the coding region, compared to variant 4. The resulting isoform (1) has a shorter and distinct N-terminus, compared to isoform 4.

Source sequence(s)

AK124594, U51477

Consensus CDS

CCDS7918.1

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000343065, OTTHUMP00000232889, ENST00000343674, OTTHUMT00000389770

Conserved Domains (6) summary

cd00204
Location:811 – 927
Blast Score: 216

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:310 – 633
Blast Score: 195

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:189 – 248
Blast Score: 108

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:811 – 905
Blast Score: 179

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:314 – 435
Blast Score: 453

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:463 – 620
Blast Score: 626

DAGK_acc; Diacylglycerol kinase accessory domain
NM_201533.3 → NP_963291.2 diacylglycerol kinase zeta isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) has an alternate 5' sequence including the coding region, lacks nts GAG at an alternate splice site and has extra nts CAG at another alternate splice sites in the CDS, compared to variant 4. The resulting isoform (3) has a shorter and distinct N-terminus, lacks an internal aa in one region and has an additional aa in another region, compared to isoform 4.

Source sequence(s)

AK300577, DC416889, U51477

Consensus CDS

CCDS44579.1

UniProtKB/TrEMBL

B7Z6M3

UniProtKB/Swiss-Prot

Q13574

Related

ENSP00000436273, OTTHUMP00000233028, ENST00000532868, OTTHUMT00000389998

Conserved Domains (6) summary

cd00204
Location:799 – 915
Blast Score: 216

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

COG1597
Location:298 – 621
Blast Score: 196

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

cl00040
Location:177 – 236
Blast Score: 108

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam12796
Location:799 – 893
Blast Score: 180

Ank_2; Ankyrin repeats (3 copies)

cl01255
Location:302 – 423
Blast Score: 453

DAGK_cat; Diacylglycerol kinase catalytic domain

cl02440
Location:451 – 608
Blast Score: 626

DAGK_acc; Diacylglycerol kinase accessory domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000011.9 Reference GRCh37.p5 Primary Assembly

Range

46354455..46402104

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000143.1 Alternate HuRef

Range

46061412..46109062

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC116021.5 (99698..147063)	None
genomic	CH471064.2	EAW68006.1
		EAW68007.1
		EAW68008.1
		EAW68009.1
mRNA	AB209635.1	BAD92872.1
mRNA	AK097960.1	None
mRNA	AK123378.1	BAC85599.1
mRNA	AK124594.1	BAC85894.1
mRNA	AK124693.1	None
mRNA	AK126970.1	BAC86770.1
mRNA	AK225774.1	None
mRNA	AK293615.1	BAH11548.1
mRNA	AK294888.1	BAH11915.1
mRNA	AK300577.1	BAH13309.1
mRNA	AK303322.1	BAH13942.1
mRNA	AK312398.1	None
mRNA	BC008018.1	AAH08018.1
mRNA	BC041770.1	AAH41770.1
mRNA	BU849628.1	None
mRNA	BX370257.2	None
mRNA	BX648433.1	None
mRNA	BX649190.1	CAE46208.1
mRNA	CB959911.1	None
mRNA	DC416889.1	None
mRNA	U51477.1	AAC50478.1
mRNA	U94905.1	AAB60859.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q13574.3	GenPept	UniProtKB/Swiss-Prot:Q13574
Q59F26	GenPept	UniProtKB/TrEMBL:Q59F26
Q6MZE6	GenPept	UniProtKB/TrEMBL:Q6MZE6
Q6ZT25	GenPept	UniProtKB/TrEMBL:Q6ZT25
Q6ZWA5	GenPept	UniProtKB/TrEMBL:Q6ZWA5
Q7Z5X8	GenPept	UniProtKB/TrEMBL:Q7Z5X8
Q8IVW9	GenPept	UniProtKB/TrEMBL:Q8IVW9