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YSP2 Ysp2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851926, updated on 27-Jan-2024

Summary

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Gene symbol
YSP2
Gene description
Ysp2p
Primary source
SGD:S000002734
Locus tag
YDR326C
See related
AllianceGenome:SGD:S000002734
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
LAM2; LTC4
Summary
Enables sterol binding activity and sterol transfer activity. Involved in intracellular sterol transport. Acts upstream of or within sterol transport. Located in cortical endoplasmic reticulum and mitochondrion. Orthologous to several human genes including GRAMD1A (GRAM domain containing 1A). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (1120609..1124925, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Utp4p Neighboring gene condensin subunit YCG1 Neighboring gene SCF ubiquitin ligase subunit SKP1 Neighboring gene Pex3p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Molecular basis for sterol transport by StART-like lipid transfer domains. Horenkamp FA, et al. EMBO J, 2018 Mar 15. PMID 29467216, Free PMC Article
  2. Mitochondrial matrix fragmentation as a protection mechanism of yeast Saccharomyces cerevisiae. Knorre DA, et al. Biochemistry (Mosc), 2008 Nov. PMID 19120030
  3. Ysp2 mediates death of yeast induced by amiodarone or intracellular acidification. Sokolov S, et al. Biochim Biophys Acta, 2006 Sep-Oct. PMID 16962064
  4. Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites. Tong J, et al. Proc Natl Acad Sci U S A, 2018 Jan 30. PMID 29339490, Free PMC Article
  5. Proteomic analysis reveals complex metabolic regulation in Saccharomyces cerevisiae cells against multiple inhibitors stress. Lv YJ, et al. Appl Microbiol Biotechnol, 2014 Mar. PMID 24442506

See all (53) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the beta-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
    Title: TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the β-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
  2. Ltc1 and Ltc3/4 function at the vacuole and plasma membrane, respectively, to create membrane domains that partition upstream regulators of the TORC1 and TORC2 signaling pathways to coordinate cellular stress responses with sterol homeostasis.
    Title: Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.
  3. Ysp2p function correlates with sterol binding to its StART-like domain.
    Title: A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport.
  4. Intracellular acidification results in the protonation of superoxide anion (O2-*) to form HO2, one of the most aggressive Reactive oxygen species, which in turn induces Ysp2-mediated programmed cell death.
    Title: Ysp2 mediates death of yeast induced by amiodarone or intracellular acidification.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables sterol binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sterol binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sterol transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sterol transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in apoptotic process IEA
Inferred from Electronic Annotation
more info
  
involved_in intermembrane lipid transfer IEA
Inferred from Electronic Annotation
more info
  
involved_in intracellular sterol transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular sterol transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within sterol transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cell periphery HDA PubMed 
is_active_in cortical endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cortical endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm HDA PubMed 
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum-plasma membrane contact site IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrial membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in organelle IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
Ysp2p
NP_010613.1
  • Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    1120609..1124925 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180634.1 → NP_010613.1  TPA: Ysp2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010613.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VSV9, Q06681
    UniProtKB/TrEMBL
    A0A8H8UNM6, A6ZYS9, C8Z5N9, N1P8N1
    Conserved Domains (2) summary
    cd13220
    Location:660 → 753
    PH-GRAM_GRAMDC; GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain
    pfam16016
    Location:854 → 998
    DUF4782; Domain of unknown function (DUF4782)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q06681.1

Gene LinkOut

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