AJUBA ajuba LIM protein [Homo sapiens (human)] - Gene

Summary

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Official Symbol: AJUBAprovided by HGNC
Official Full Name: ajuba LIM proteinprovided by HGNC
Primary source: HGNC:20250
See related: Ensembl:ENSG00000129474; HPRD:16426; MIM:609066; Vega:OTTHUMG00000028711
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: JUB

Genomic context

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Location :: 14q11.2

Sequence :: Chromosome: 14; NC_000014.8 (23440409..23451851, complement)

See AJUBA in Epigenomics, MapViewer

Chromosome 14 - NC_000014.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

A Rac-PAK1-Ajuba feedback loop integrates spatiotemporal signaling with actin remodeling at cell-cell contacts and stabilizes preassembled cadherin complexes.
Title: Ajuba is required for Rac activation and maintenance of E-cadherin adhesion.
Observational study of gene-disease association. (HuGE Navigator)
Title: Centrosome-related genes, genetic variation, and risk of breast cancer.
Ajuba contains functional nuclear-receptor interacting motifs and selectively interacts with retinoic acid receptors and rexinoid receptor subtypes.
Title: LIM protein Ajuba functions as a nuclear receptor corepressor and negatively regulates retinoic acid signaling.
Ajuba is a microtubule-associated protein that collaborates with Aurora B and BUBR1 at the metaphase-anaphase transition and this may be important to ensure proper chromosome segregation.
Title: Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase.
Ajuba is utilized as a corepressor selectively on Gfi1 target genes
Title: Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor.
Ajuba promoted GSK-3beta-mediated phosphorylation of beta-catenin by reinforcing the association between beta-catenin and GSK-3beta.
Title: Ajuba negatively regulates the Wnt signaling pathway by promoting GSK-3beta-mediated phosphorylation of beta-catenin.
Ajuba is a new cytosolic component of the IL-1 signaling pathway modulating IL-1-induced NF-kappaB activation by influencing the assembly and activity of the aPKC/p62/TRAF6 multiprotein signaling complex
Title: The LIM protein Ajuba influences interleukin-1-induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex.
in addition to its effects upon Rac activity, Ajuba can also influence cell migration through regulation of PI(4,5)P2 synthesis through direct activation of PIPKIalpha enzyme activity
Title: The LIM protein Ajuba regulates phosphatidylinositol 4,5-bisphosphate levels in migrating cells through an interaction with and activation of PIPKI alpha.
Ajuba contributes to the bridging of the cadherin adhesive complexes to the actin cytoskeleton and as such contribute to the formation or strengthening of cadherin-mediated cell-cell adhesion.
Title: The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
Ajuba	P68133	ACTA1	HPRD	PubMed
Ajuba	Q9NWT8	AURKAIP1	HPRD	PubMed
Ajuba	P56945	BCAR1	HPRD	PubMed
Ajuba	P35221	CTNNA1	HPRD	PubMed
Ajuba	Growth factor independent 1B	GFI1B	HPRD	PubMed
Ajuba	P62993	GRB2	HPRD	PubMed
Ajuba	Q9NRM7	LATS2	HPRD	PubMed
Ajuba	P43004	SLC1A2	HPRD	PubMed
BioGRID:124393	BioGRID:114646	AURKB	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:124393	BioGRID:107166	BUB1B	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:125184	EGLN2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:125185	EGLN3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:113924	GFI1B	BioGRID	PubMed	Two-hybrid
BioGRID:124393	BioGRID:117727	LATS2	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Two-hybrid
BioGRID:124393	BioGRID:110124	LCK	BioGRID	PubMed	Two-hybrid
BioGRID:124393	BioGRID:111576	PRKCZ	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:115688	PRMT5	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:111849	RARA	BioGRID	PubMed	Affinity Capture-Western; Co-fractionation; Reconstituted Complex
BioGRID:124393	BioGRID:111851	RARG	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:112170	RXRG	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:109072	SFN	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:124393	BioGRID:112397	SLC1A2	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:124393	BioGRID:112499	SNAI1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:114397	SQSTM1	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:124393	BioGRID:113041	TRAF6	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:124393	BioGRID:113269	VHL	BioGRID	PubMed	Affinity Capture-Western
BioGRID:124393	BioGRID:113366	YWHAZ	BioGRID	PubMed	Affinity Capture-Western

Pathways from BioSystems

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Aurora A signaling, organism-specific biosystem (from Pathway Interaction Database)
Aurora A signaling, organism-specific biosystem
Aurora A signaling
E-cadherin signaling in keratinocytes, organism-specific biosystem (from Pathway Interaction Database)
E-cadherin signaling in keratinocytes, organism-specific biosystem
E-cadherin signaling in keratinocytes
Hippo signaling pathway, organism-specific biosystem (from KEGG)
Hippo signaling pathway, organism-specific biosystemHippo signaling is an evolutionarily conserved signaling pathway that controls organ size from flies to humans. In humans and mice, the pathway consists of the MST1 and MST2 kinases, their cofactor S...
Hippo signaling pathway, conserved biosystem (from KEGG)
Hippo signaling pathway, conserved biosystemHippo signaling is an evolutionarily conserved signaling pathway that controls organ size from flies to humans. In humans and mice, the pathway consists of the MST1 and MST2 kinases, their cofactor S...

General gene information

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Markers

WI-17421 (e-PCR)
- UniSTS:62850

RH47855 (e-PCR)
- UniSTS:65414

RH68535 (e-PCR)
- UniSTS:42171

G59876 (e-PCR)
- UniSTS:137159

MARC_11631-11632:1002817108:1 (e-PCR)
- UniSTS:267252

Homology

Homologs of the AJUBA gene: The AJUBA gene is conserved in Rhesus monkey, dog, cow, mouse, rat, and zebrafish.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
actin filament binding	IEA Inferred from Electronic Annotation more info
alpha-catenin binding	IDA Inferred from Direct Assay more info	PubMed
chromatin binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
transcription corepressor activity	IDA Inferred from Direct Assay more info	PubMed
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
calcium-dependent cell-cell adhesion	IEA Inferred from Electronic Annotation more info
cell cycle	IEA Inferred from Electronic Annotation more info
cellular protein localization	IEA Inferred from Electronic Annotation more info
focal adhesion assembly	IEA Inferred from Electronic Annotation more info
gene silencing by miRNA	IMP Inferred from Mutant Phenotype more info
glycerophospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
lamellipodium assembly	IEA Inferred from Electronic Annotation more info
negative regulation of hippo signaling cascade	IDA Inferred from Direct Assay more info
negative regulation of kinase activity	IEA Inferred from Electronic Annotation more info
negative regulation of transcription from RNA polymerase II promoter	IEA Inferred from Electronic Annotation more info
positive regulation of I-kappaB kinase/NF-kappaB cascade	IEA Inferred from Electronic Annotation more info
positive regulation of MAP kinase activity	IEA Inferred from Electronic Annotation more info
positive regulation of cellular biosynthetic process	IEA Inferred from Electronic Annotation more info
positive regulation of gene silencing by miRNA	IMP Inferred from Mutant Phenotype more info
positive regulation of protein complex assembly	IDA Inferred from Direct Assay more info	PubMed
regulation of GTPase activity	IEA Inferred from Electronic Annotation more info
regulation of cell migration	IEA Inferred from Electronic Annotation more info
regulation of cellular response to hypoxia	IDA Inferred from Direct Assay more info
response to hypoxia	IDA Inferred from Direct Assay more info
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
wound healing, spreading of epidermal cells	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
cytoplasmic mRNA processing body	IDA Inferred from Direct Assay more info
cytosol	IEA Inferred from Electronic Annotation more info
focal adhesion	IEA Inferred from Electronic Annotation more info
lamellipodium	IEA Inferred from Electronic Annotation more info
microtubule organizing center	IEA Inferred from Electronic Annotation more info
nucleus	IDA Inferred from Direct Assay more info	PubMed
plasma membrane	IEA Inferred from Electronic Annotation more info
transcription factor complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: LIM domain-containing protein ajuba

Names: LIM domain-containing protein ajuba; protein ajuba; jub, ajuba homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_032876.4 → NP_116265.1 LIM domain-containing protein ajuba isoform 1

Status: VALIDATED

Source sequence(s)

AK025567, BC034968, BU543883, CB105505

Consensus CDS

CCDS9581.1

UniProtKB/Swiss-Prot

Q96IF1

Related

ENSP00000262713, OTTHUMP00000027924, ENST00000262713, OTTHUMT00000071685

Conserved Domains (3) summary

cd09352
Location:338 – 391
Blast Score: 267

LIM1_Ajuba_like; The first LIM domain of Ajuba-like proteins

cd09355
Location:403 – 455
Blast Score: 278

LIM2_Ajuba_like; The second LIM domain of Ajuba-like proteins

cd09438
Location:463 – 524
Blast Score: 311

LIM3_Ajuba_like; The third LIM domain of Ajuba-like proteins
NM_198086.1 → NP_932352.1 LIM domain-containing protein ajuba isoform 2

Status: VALIDATED

Source sequence(s)

AK025567, AK096128, BC034968, CB105505

Consensus CDS

CCDS9582.1

UniProtKB/Swiss-Prot

Q96IF1

Related

ENSP00000380543, OTTHUMP00000164552, ENST00000397388, OTTHUMT00000257240

Conserved Domains (2) summary

cd09438
Location:46 – 107
Blast Score: 270

LIM3_Ajuba_like; The third LIM domain of Ajuba-like proteins

cl02475
Location:1 – 38
Blast Score: 176

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000014.8 Reference GRCh37.p10 Primary Assembly

Range

23440409..23451851, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000146.1 Alternate HuRef

Range

3557642..3569085, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018925.1 Alternate CHM1_1.0

Range

4437495..4448938, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01061298.1 (408..11851)	None
genomic	AL132780.5 (74996..86438)	None
genomic	AMYH01005601.1 (26240..37683)	None
genomic	CH471078.2	EAW66204.1
		EAW66205.1
mRNA	AK025567.1	None
mRNA	AK096128.1	BAG53217.1
mRNA	AY169959.1	AAO37641.1
mRNA	BC007580.1	AAH07580.1
mRNA	BC016733.1	None
mRNA	BC034968.2	AAH34968.1
mRNA	BU543883.1	None
mRNA	CB105505.1	None
other-genetic	DQ893513.2	ABM84439.1
other-genetic	DQ896486.2	ABM87485.1