CDC42BPA CDC42 binding protein kinase alpha (DMPK-like) [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: CDC42BPAprovided by HGNC
Official Full Name: CDC42 binding protein kinase alpha (DMPK-like)provided by HGNC
Primary source: HGNC:1737
Locus tag: RP5-1087E8.4
See related: Ensembl:ENSG00000143776; HPRD:04562; MIM:603412; Vega:OTTHUMG00000037618
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MRCK; MRCKA; PK428; FLJ23347; KIAA0451; DKFZp686L1738; DKFZp686P1738
Summary: The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008]

Help top of page

Genomic context

Location :: 1q42.11

Sequence :: Chromosome: 1; NC_000001.10 (227177566..227505826, complement)

See CDC42BPA in Epigenomics, MapViewer

Chromosome 1 - NC_000001.10 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that chelerythrine blocks cellular activity of MRCK resulted in the specific loss of NM II-associated MLC phosphorylation in the lamella, and the consequential suppression of cell migration.
Title: Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase.
MRCKalpha takes part in transferrin (Tf)-iron uptake, probably via regulation of Tf- TfR endocytosis/endosome trafficking that is dependent on the cellular cytoskeleton.
Title: Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake.
analysis of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta
Title: Characterization of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta.
a Cdc42-MRCK signal mediates myosin-dependent cell motility and there is convergence between Rho and Cdc42 signalling
Title: Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion.
findings report that the Notch1 gene is a p53 target in human keratinocytes with a role in tumor suppression of this cell type through negative regulation of the ROCK1/2 and MRCKalpha kinases
Title: Notch1 is a p53 target gene involved in human keratinocyte tumor suppression through negative regulation of ROCK1/2 and MRCKalpha kinases.
The level of MRCKalpha mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway.
Title: A novel iron responsive element in the 3'UTR of human MRCKalpha.
Class II histone deacetylases are directly recruited by BCL6 transcriptional repressor
Title: Class II histone deacetylases are directly recruited by BCL6 transcriptional repressor.

Submit: New GeneRIF Correction

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
Q5VT25	Q5VT25	CDC42BPA	HPRD	PubMed
Q5VT25	P53667	LIMK1	HPRD	PubMed
Q5VT25	P53671	LIMK2	HPRD	PubMed
Q5VT25	P10916	MYL2	HPRD	PubMed
Q5VT25	P24844	MYL9	HPRD	PubMed
Q5VT25	Protein phosphatase 1, regulatory subunit 12C	PPP1R12C	HPRD	PubMed
BioGRID:114051	BioGRID:107433	CDC42	BioGRID	PubMed	Two-hybrid
BioGRID:114051	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IDA Inferred from Direct Assay more info	PubMed
identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
small GTPase regulator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
actin cytoskeleton reorganization	IDA Inferred from Direct Assay more info	PubMed
actomyosin structure organization	IMP Inferred from Mutant Phenotype more info	PubMed
cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
intracellular signal transduction	IEA Inferred from Electronic Annotation more info
protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
actomyosin	IDA Inferred from Direct Assay more info	PubMed
cell leading edge	ISS Inferred from Sequence or Structural Similarity more info
cell-cell junction	ISS Inferred from Sequence or Structural Similarity more info
cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: serine/threonine-protein kinase MRCK alpha

Names: serine/threonine-protein kinase MRCK alpha; MRCK alpha; DMPK-like alpha; ser-thr protein kinase PK428; CDC42 binidng protein kinase beta; myotonic dystrophy protein kinase-like alpha; CDC42-binding protein kinase alpha (DMPK-like); myotonic dystrophy kinase-related CDC42-binding kinase alpha; myotonic dystrophy kinase-related CDC42-binding protein kinase alpha; ser-thr protein kinase related to the myotonic dystrophy protein kinase

NP_003598.2

EC 2.7.11.1

NP_055641.3

EC 2.7.11.1

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003607.3 → NP_003598.2 serine/threonine-protein kinase MRCK alpha isoform B

Status: REVIEWED

Description

Transcript Variant: This variant (B) encodes the longer isoform (B).

Source sequence(s)

AJ518975, AL353689, AL627308, BC050335, BF765241, BQ232716, BX648219

Consensus CDS

CCDS1558.1

UniProtKB/Swiss-Prot

Q5VT25

Related

ENSP00000355731, OTTHUMP00000035727, ENST00000366769, OTTHUMT00000091696

Conserved Domains (8) summary

cd00029
Location:1000 – 1049
Blast Score: 151

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00132
Location:1556 – 1591
Blast Score: 105

CRIB; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...

COG0419
Location:442 – 986
Blast Score: 215

SbcC; ATPase involved in DNA repair [DNA replication, recombination, and repair]

pfam08826
Location:881 – 941
Blast Score: 220

DMPK_coil; DMPK coiled coil domain like

cd05597
Location:75 – 405
Blast Score: 1940

STKc_DMPK_like; Catalytic domain of Myotonic Dystrophy protein kinase-like Protein Serine/Threonine Kinases

cd01243
Location:1069 – 1190
Blast Score: 633

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

smart00220
Location:77 – 343
Blast Score: 703

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl02434
Location:1219 – 1478
Blast Score: 675

CNH; CNH domain
NM_014826.4 → NP_055641.3 serine/threonine-protein kinase MRCK alpha isoform A

Status: REVIEWED

Description

Transcript Variant: This variant (A) lacks a coding segment compared to variant B, resulting in an isoform (A) that lacks an internal region, as compared to isoform B.

Source sequence(s)

AJ518976, AL353689, AL627308, BC050335, BF765241, BQ232716, BX648219

Consensus CDS

CCDS1559.1

UniProtKB/Swiss-Prot

Q5VT25

Related

ENSP00000355729, OTTHUMP00000035728, ENST00000366767, OTTHUMT00000091697

Conserved Domains (8) summary

cd00029
Location:919 – 968
Blast Score: 151

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00132
Location:1475 – 1510
Blast Score: 105

CRIB; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...

COG0419
Location:442 – 905
Blast Score: 165

SbcC; ATPase involved in DNA repair [DNA replication, recombination, and repair]

pfam08826
Location:800 – 860
Blast Score: 227

DMPK_coil; DMPK coiled coil domain like

cd05597
Location:75 – 405
Blast Score: 1938

STKc_DMPK_like; Catalytic domain of Myotonic Dystrophy protein kinase-like Protein Serine/Threonine Kinases

cd01243
Location:988 – 1109
Blast Score: 634

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

smart00220
Location:77 – 343
Blast Score: 704

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl02434
Location:1138 – 1397
Blast Score: 675

CNH; CNH domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000001.10 Reference GRCh37.p5 Primary Assembly

Range

227177566..227505826, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000133.1 Alternate HuRef

Range

197694156..198022367, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AL353689.26	CAI19107.1
		CAI19108.1
		CAI19109.1
		CAI19110.1
		CAI19111.1
		CAI19112.1
		CAI19113.1
genomic	AL353689.26	CAI19107.1
		CAI19108.1
		CAI19109.1
		CAI19110.1
		CAI19111.1
		CAI19112.1
		CAI19113.1
genomic	AL451047.7	CAH71183.1
		CAH71184.1
		CAH71185.1
genomic	AL627308.20	CAH71336.1
		CAH71337.1
		CAH71338.1
genomic	CH471098.1	EAW69806.1
		EAW69807.1
		EAW69808.1
		EAW69809.1
mRNA	AB007920.2	BAA32296.2
mRNA	AB208968.1	BAD92205.1
mRNA	AF250871.1	AAF66437.1
mRNA	AJ518975.1	CAD57745.1
mRNA	AJ518976.1	CAD57746.1
mRNA	AK027000.1	BAB15619.1
mRNA	AK130237.1	None
mRNA	AY166586.1	AAN86031.1
mRNA	BC008677.1	None
mRNA	BC038993.1	None
mRNA	BC047547.1	None
mRNA	BC050335.1	None
mRNA	BC127831.1	None
mRNA	BC136333.1	AAI36334.1
mRNA	BF765241.1	None
mRNA	BQ232716.1	None
mRNA	BX648219.1	None
mRNA	BX648693.1	CAI45998.1
mRNA	CR933723.1	CAI46252.1
mRNA	U59305.1	AAB37126.1
other-genetic	BC156892.1	AAI56893.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q5HYB0	GenPept	UniProtKB/TrEMBL:Q5HYB0
Q5T799	GenPept	UniProtKB/TrEMBL:Q5T799
Q5T7A0	GenPept	UniProtKB/TrEMBL:Q5T7A0
Q5T7A7	GenPept	UniProtKB/TrEMBL:Q5T7A7
Q5VT25.1	GenPept	UniProtKB/Swiss-Prot:Q5VT25
Q8IWQ7	GenPept	UniProtKB/TrEMBL:Q8IWQ7
Q9H5K7	GenPept	UniProtKB/TrEMBL:Q9H5K7
Q9NYF6	GenPept	UniProtKB/TrEMBL:Q9NYF6