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    NCK2 NCK adaptor protein 2 [ Homo sapiens ]

    Gene ID: 8440, updated on 11-May-2012

    Summary

    Official Symbol
    NCK2provided by HGNC
    Official Full Name
    NCK adaptor protein 2provided by HGNC
    Primary source
    HGNC:7665
    See related
    Ensembl:ENSG00000071051; HPRD:05378; MIM:604930; Vega:OTTHUMG00000153116
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    GRB4; NCKbeta
    Summary
    This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
    Annotation information
    Annotation category: partial on reference assembly

    Genomic context

    Location :
    2q12
    Sequence :
    Chromosome: 2; NC_000002.11 (106432885..106510730)
    See NCK2 in Epigenomics, MapViewer

    Chromosome 2 - NC_000002.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC100506494 Neighboring gene four and a half LIM domains 2 Neighboring gene uncharacterized LOC285000 Neighboring gene uncharacterized LOC100506513 Neighboring gene chromosome 2 open reading frame 40 Neighboring gene UDP-glucuronate decarboxylase 1

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_003572.2 FGFR1    BIND  PubMed An unspecified isoform of FGFR1 interacts with NCK2. This interaction was modeled on a demonstrated interaction between FGFR1 from an unspecified species and human NCK2. 
    NP_003572.2 NP_004978.2 LIMS1    BIND  PubMed Nck-2 interacts with PINCH 
    O43639 Q8IZP0 ABI1    HPRD  PubMed  
    O43639 Q9NYB9 ABI2    HPRD  PubMed  
    O43639 P54259 ATN1    HPRD  PubMed  
    O43639 O15169 AXIN1    HPRD  PubMed  
    O43639 P30530 AXL    HPRD  PubMed  
    O43639 Q13191 CBLB    HPRD  PubMed  
    O43639 P07766 CD3E    HPRD  PubMed  
    O43639 Q16630 CPSF6    HPRD  PubMed  
    O43639 Pre-mRNA cleavage factor I, 59 kDa subunit CPSF7    HPRD  PubMed  
    O43639 Q08345 DDR1    HPRD  PubMed  
    O43639 Q05193 DNM1    HPRD  PubMed  
    O43639 Q14185 DOCK1    HPRD  PubMed  
    O43639 Q99704 DOK1    HPRD  PubMed  
    O43639 P26641 EEF1G    HPRD  PubMed  
    O43639 P98172 EFNB1    HPRD  PubMed  
    O43639 P52799 EFNB2    HPRD  PubMed  
    O43639 P00533 EGFR    HPRD  PubMed  
    O43639 P11362 FGFR1    HPRD  PubMed  
    O43639 P51116 FXR2    HPRD  PubMed  
    O43639 P61978 HNRNPK    HPRD  PubMed  
    O43639 Q5T5P2 KIAA1217    HPRD  PubMed  
    O43639 P48059 LIMS1    HPRD  PubMed  
    O43639 Q9NZQ3 NCKIPSD    HPRD  PubMed  
    O43639 O60500 NPHS1    HPRD  PubMed  
    O43639 Q16620 NTRK2    HPRD  PubMed  
    O43639 Q13153 PAK1    HPRD  PubMed  
    O43639 P09619 PDGFRB    HPRD  PubMed  
    O43639 Q16513 PKN2    HPRD  PubMed  
    O43639 Q05397 PTK2    HPRD  PubMed  
    O43639 Q15427 SF3B4    HPRD  PubMed  
    O43639 Q07889 SOS1    HPRD  PubMed  
    O43639 Q15654 TRIP6    HPRD  PubMed  
    O43639 Q9Y2W2 WBP11    HPRD  PubMed  
    O43639 Q8TF74 WIPF2    HPRD  PubMed  
    BioGRID:114020 BioGRID:115454 ABI2    BioGRID  PubMed Two-hybrid 
    NP_068713.2 AXL    BIND  PubMed Axl interacts with an unspecified isoform of Nck2. 
    BioGRID:114020 BioGRID:107316 CBLB    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:107354 CD3E    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:114020 BioGRID:116238 CPSF6    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:122957 CPSF7    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:108128 DOCK1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:114020 BioGRID:108131 DOK1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:114020 BioGRID:108257 EEF1G    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:108276 EGFR    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:114020 BioGRID:114890 FXR2    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:109431 HNRNPK    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:109824 ILK    BioGRID  PubMed Reconstituted Complex 
    BioGRID:114020 BioGRID:109874 IRS1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:114020 BioGRID:121111 KIAA1217    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:110175 LIMS1    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:114020 BioGRID:111629 MASP1    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:119583 NCKIPSD    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:110970 NTRK2    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:114020 BioGRID:111095 PAK1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:114020 BioGRID:111185 PDGFRB    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:114020 BioGRID:111572 PKN2    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:114020 BioGRID:111719 PTK2    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:114020 BioGRID:202089 Pdgfrb    BioGRID  PubMed Reconstituted Complex 
    BioGRID:114020 BioGRID:115554 SF3B4    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:112537 SOS1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:114020 BioGRID:113056 TRIP6    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:119702 WBP11    BioGRID  PubMed Two-hybrid 
    BioGRID:114020 BioGRID:127043 WIPF2    BioGRID  PubMed Affinity Capture-Western 

    General gene information

    Markers

    Homology

    Pathways from BioSystems

    • Activation of Rac, organism-specific biosystem (from REACTOME)
      Activation of Rac, organism-specific biosystemA low level of Rac activity is essential to maintain axon outgrowth and Robo activation recruits Sos, a dual specificity GEF to the plasma membrane via Dock to activate Rac during midline repulsion.
    • Axon guidance, organism-specific biosystem (from KEGG)
      Axon guidance, organism-specific biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
    • Axon guidance, conserved biosystem (from KEGG)
      Axon guidance, conserved biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
    • Axon guidance, organism-specific biosystem (from REACTOME)
      Axon guidance, organism-specific biosystemAxon guidance / axon pathfinding is the process by which neurons send out axons to reach the correct targets. Growing axons have a highly motile structure at the growing tip called the growth cone, w...
    • Cell-Cell communication, organism-specific biosystem (from REACTOME)
      Cell-Cell communication, organism-specific biosystemCell-to-Cell communication is crucial for multicellular organisms because it allows organisms to coordinate the activity of their cells. Some cell-to-cell communication requires direct cell-cell cont...
    • Developmental Biology, organism-specific biosystem (from REACTOME)
      Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
    • Downstream signal transduction, organism-specific biosystem (from REACTOME)
      Downstream signal transduction, organism-specific biosystemThe role of autophosphorylation sites on PDGF receptors are to provide docking sites for downstream signal transduction molecules which contain SH2 domains. The SH2 domain is a conserved motif of aro...
    • EGFR1 Signaling Pathway, organism-specific biosystem (from WikiPathways)
      EGFR1 Signaling Pathway, organism-specific biosystemThe androgen receptor is a member of the nuclear receptor family of ligand activated transcription factors. These receptors bind to steroid hormones, thyroid hormone, retinoids and vitamin D among ot...
    • Ephrin B reverse signaling, organism-specific biosystem (from Pathway Interaction Database)
      Ephrin B reverse signaling, organism-specific biosystem
      Ephrin B reverse signaling
    • ErbB signaling pathway, organism-specific biosystem (from KEGG)
      ErbB signaling pathway, organism-specific biosystemThe ErbB family of receptor tyrosine kinases (RTKs) couples binding of extracellular growth factor ligands to intracellular signaling pathways regulating diverse biologic responses, including prolife...
    • ErbB signaling pathway, conserved biosystem (from KEGG)
      ErbB signaling pathway, conserved biosystemThe ErbB family of receptor tyrosine kinases (RTKs) couples binding of extracellular growth factor ligands to intracellular signaling pathways regulating diverse biologic responses, including prolife...
    • IGF1 pathway, organism-specific biosystem (from Pathway Interaction Database)
      IGF1 pathway, organism-specific biosystem
      IGF1 pathway
    • Insulin Pathway, organism-specific biosystem (from Pathway Interaction Database)
      Insulin Pathway, organism-specific biosystem
      Insulin Pathway
    • Nephrin interactions, organism-specific biosystem (from REACTOME)
      Nephrin interactions, organism-specific biosystemNephrin is a member of the Super-IgG-Molecule family and is most prominently expressed in kidney podocytes. It is a major if not the most important structural component of the slit diaphragm, a modif...
    • Nephrin/Neph1 signaling in the kidney podocyte, organism-specific biosystem (from Pathway Interaction Database)
      Nephrin/Neph1 signaling in the kidney podocyte, organism-specific biosystem
      Nephrin/Neph1 signaling in the kidney podocyte
    • PDGFR-beta signaling pathway, organism-specific biosystem (from Pathway Interaction Database)
      PDGFR-beta signaling pathway, organism-specific biosystem
      PDGFR-beta signaling pathway
    • Pathogenic Escherichia coli infection, organism-specific biosystem (from KEGG)
      Pathogenic Escherichia coli infection, organism-specific biosystemEnteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections [DS:H00278 H00277] is induction o...
    • Pathogenic Escherichia coli infection, conserved biosystem (from KEGG)
      Pathogenic Escherichia coli infection, conserved biosystemEnteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections [DS:H00278 H00277] is induction o...
    • Reelin signaling pathway, organism-specific biosystem (from Pathway Interaction Database)
      Reelin signaling pathway, organism-specific biosystem
      Reelin signaling pathway
    • Signal Transduction, organism-specific biosystem (from REACTOME)
      Signal Transduction, organism-specific biosystemSignal transduction is a process in which extracellular signals elicit changes in cell state and activity. Transmembrane receptors sense changes in the cellular environment by binding ligands, such a...
    • Signaling by PDGF, organism-specific biosystem (from REACTOME)
      Signaling by PDGF, organism-specific biosystemPlatelet-derived Growth Factor (PDGF) is a potent stimulator of growth and motility of connective tissue cells such as fibroblasts and smooth muscle cells as well as other cells such as capillary end...
    • Signaling by Robo receptor, organism-specific biosystem (from REACTOME)
      Signaling by Robo receptor, organism-specific biosystemThe Roundabout (Robo) family encodes transmembrane receptors that regulate axonal guidance and cell migration. The major function of the Robo receptors is to mediate repulsion of the navigating growt...
    • Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met), organism-specific biosystem (from Pathway Interaction Database)
      Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met), organism-specific biosystem
      Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
    • T cell receptor signaling pathway, organism-specific biosystem (from KEGG)
      T cell receptor signaling pathway, organism-specific biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...
    • T cell receptor signaling pathway, conserved biosystem (from KEGG)
      T cell receptor signaling pathway, conserved biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    cytoskeletal adaptor activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    receptor signaling complex scaffold activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    T cell activation NAS
    Non-traceable Author Statement
    more info
    PubMed 
    actin filament organization IEA
    Inferred from Electronic Annotation
    more info
     
    axon guidance TAS
    Traceable Author Statement
    more info
     
    cell migration IEA
    Inferred from Electronic Annotation
    more info
     
    epidermal growth factor receptor signaling pathway TAS
    Traceable Author Statement
    more info
    PubMed 
    lamellipodium assembly IEA
    Inferred from Electronic Annotation
    more info
     
    negative regulation of cell proliferation TAS
    Traceable Author Statement
    more info
    PubMed 
    positive regulation of T cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of actin filament polymerization IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of transcription from RNA polymerase II promoter IDA
    Inferred from Direct Assay
    more info
    PubMed 
    regulation of epidermal growth factor-activated receptor activity TAS
    Traceable Author Statement
    more info
    PubMed 
    regulation of translation IEA
    Inferred from Electronic Annotation
    more info
     
    signal complex assembly NAS
    Non-traceable Author Statement
    more info
    PubMed 
    signal transduction TAS
    Traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    cytoplasm NAS
    Non-traceable Author Statement
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
     
    vesicle membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    cytoplasmic protein NCK2
    Names
    cytoplasmic protein NCK2
    SH2/SH3 adaptor protein NCK-beta
    growth factor receptor-bound protein 4
    noncatalytic region of tyrosine kinase, beta

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001004720.2NP_001004720.1  cytoplasmic protein NCK2 isoform A

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (A).
      Source sequence(s)
      AC009505, BC000103, BU738133, DB456958
      Consensus CDS
      CCDS33266.1
      UniProtKB/Swiss-Prot
      O43639
      Related
      ENSP00000377018, OTTHUMP00000203153, ENST00000393349, OTTHUMT00000329635
      Conserved Domains (2) summary
      cd00174
      Location:199252
      Blast Score: 160
      SH3; Src homology 3 domains; SH3 domains bind to proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs; they play a role in the regulation of enzymes by intramolecular interactions, changing the subcellular localization of ...
      cd10409
      Location:283380
      Blast Score: 459
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    2. NM_001004722.3NP_001004722.1  cytoplasmic protein NCK2 isoform B

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks an exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A.
      Source sequence(s)
      AC009505, AC010978, BG480643
      UniProtKB/TrEMBL
      E7ERP6
      Related
      ENSP00000410428, ENST00000451463
      Conserved Domains (1) summary
      cd00174
      Location:658
      Blast Score: 143
      SH3; Src homology 3 domains; SH3 domains bind to proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs; they play a role in the regulation of enzymes by intramolecular interactions, changing the subcellular localization of ...
    3. NM_003581.4NP_003572.2  cytoplasmic protein NCK2 isoform A

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A.
      Source sequence(s)
      AC009505, AC010978, BC007195
      Consensus CDS
      CCDS33266.1
      UniProtKB/Swiss-Prot
      O43639
      Related
      ENSP00000233154, OTTHUMP00000203152, ENST00000233154, OTTHUMT00000329634
      Conserved Domains (2) summary
      cd00174
      Location:199252
      Blast Score: 160
      SH3; Src homology 3 domains; SH3 domains bind to proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs; they play a role in the regulation of enzymes by intramolecular interactions, changing the subcellular localization of ...
      cd10409
      Location:283380
      Blast Score: 459
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000002.11 Reference GRCh37.p5 Primary Assembly

      Range
      106432885..106510730
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000134.1 Alternate HuRef

      Range
      100201822..100279928
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC009505.3 AAY24332.1
    genomic AC010978.8 AAY14937.1
    genomic CH471127.2 EAX01753.1
      EAX01754.1
    mRNA AF043119.1 AAC04831.1
    mRNA AF047487.1 AAC80284.1
    mRNA BC000103.2 AAH00103.1
    mRNA BC007195.1 AAH07195.1
    mRNA BC033176.1 None
    mRNA BG480643.1 None
    mRNA BM555604.1 None
    mRNA BM920530.1 None
    mRNA BU738133.1 None
    mRNA CB959523.1 None
    mRNA DB456958.1 None
    Protein Accession Links
    GenPept Link UniProtKB Link
    O43639.2 GenPept UniProtKB/Swiss-Prot:O43639
    Q53T91 GenPept UniProtKB/TrEMBL:Q53T91
    Q53TG4 GenPept UniProtKB/TrEMBL:Q53TG4

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