Usp8 ubiquitin specific peptidase 8 [Mus musculus] - Gene

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Summary

Official Symbol: Usp8provided by MGI
Official Full Name: ubiquitin specific peptidase 8provided by MGI
Primary source: MGI:1934029
Locus tag: RP23-130L11.1
See related: Ensembl:ENSMUSG00000027363; Vega:OTTMUSG00000015864
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ubpy; AI574262; AW557536; mKIAA0055

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Genomic context

Location :: 2 F1; 2

Sequence :: Chromosome: 2; NC_000068.7 (126707328..126759314)

See Usp8 in Epigenomics, MapViewer

Chromosome 2 - NC_000068.7 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The spatio-temporal expression of mUBPy suggests that the enzyme may be involved in neuroregulatory processes during embryogenesis.
Title: Expression pattern of mUBPy in the brain and sensory organs of mouse during embryonic development.
a role for the USP8.STAM complex as a protective mechanism regulating early endosomal sorting of EGFR between pathways destined for lysosomal degradation and recycling.
Title: Regulation of epidermal growth factor receptor ubiquitination and trafficking by the USP8·STAM complex.
USP8 was able to interact with spermatid endosomal-sorting complex required for transport-0 and microtubule structures; USP8 can directly link, via its MIT domain, the labeled vesicles/developing acrosome to microtubules.
Title: USP8, a regulator of endosomal sorting, is involved in mouse acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.
in wobbler testis expression of mouse ubiquitin-specific processing protease (mUBPy)is up-regulated, while a differential sorting of the protein characterizes wobbler spermatids where acrosome formation is impaired
Title: Expression and localization of the deubiquitinating enzyme mUBPy in wobbler mouse testis during spermiogenesis.
Data indicate a novel mechanism where diacylglycerol kinase delta and protein kinase Calpha modulate the levels of ubiquitinated epidermal growth factor receptors through Akt and ubiquitin-specific protease 8.
Title: Diacylglycerol kinase delta and protein kinase C(alpha) modulate epidermal growth factor receptor abundance and degradation through ubiquitin-specific protease 8.
Moderate expression of mouse UBPy was found in the amygdaloid complex, supraoptic nucleus, arcuate and ventromedial nuclei of hypothalamus, lateral hypothalamic area and lateral and reticular part of the substantia nigra.
Title: Expression of the deubiquitinating enzyme mUBPy in the mouse brain.
UBPY is catalytically inhibited in a phosphorylation-dependent manner by 14-3-3s during the interphase, and this regulation is cancelled in the M phase.
Title: 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase.
UBPY-mediated EGFR de-ubiquitination promotes EGFR degradation
Title: UBPY-mediated epidermal growth factor receptor (EGFR) de-ubiquitination promotes EGFR degradation.
Results indicate that Nrdp1 is a specific target for the USP8 deubiquitinating enzyme and are consistent with a model where USP8 augments Nrdp1 activity by mediating its stabilization.
Title: Stabilization of the E3 ubiquitin ligase Nrdp1 by the deubiquitinating enzyme USP8.
deubiquitinating enzyme specifically expressed in testis, associates with the acrosome and centrosome in mouse germ cells
Title: mUBPy and MSJ-1, a deubiquitinating enzyme and a molecular chaperone specifically expressed in testis, associate with the acrosome and centrosome in mouse germ cells.

Submit: New GeneRIF Correction See all (13)

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (1) 1 citation
Targeted (knock-out) (1) 1 citation
Gene trapped (1)

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_062703.2	NP_034945.1	Grap2	BIND	PubMed	Gads interacts with UBPY.
BioGRID:220000	BioGRID:200447	Dnajb3	BioGRID	PubMed	Far Western; Reconstituted Complex
BioGRID:220000	BioGRID:108276	EGFR	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:220000	BioGRID:202600	Rasgrf1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:220000	BioGRID:212296	Rnf41	BioGRID	PubMed	Affinity Capture-Western
BioGRID:220000	BioGRID:207904	Stam2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:220000	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western
BioGRID:220000	BioGRID:113363	YWHAE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:220000	BioGRID:113364	YWHAG	BioGRID	PubMed	Affinity Capture-MS
BioGRID:220000	BioGRID:113366	YWHAZ	BioGRID	PubMed	Affinity Capture-MS
BioGRID:220000	BioGRID:204619	Ywhae	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Reconstituted Complex

Function	Evidence Code	Pubs
SH3 domain binding	IEA Inferred from Electronic Annotation more info
cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
peptidase activity	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
ubiquitin thiolesterase activity	IEA Inferred from Electronic Annotation more info
ubiquitin-specific protease activity	IDA Inferred from Direct Assay more info	PubMed
ubiquitin-specific protease activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
Ras protein signal transduction	IPI Inferred from Physical Interaction more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
endosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
endosome organization	ISO Inferred from Sequence Orthology more info
protein K48-linked deubiquitination	ISO Inferred from Sequence Orthology more info
protein K63-linked deubiquitination	ISO Inferred from Sequence Orthology more info
protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
protein deubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
proteolysis	IEA Inferred from Electronic Annotation more info
ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
cytosol	IDA Inferred from Direct Assay more info	PubMed
cytosol	ISO Inferred from Sequence Orthology more info
early endosome	ISO Inferred from Sequence Orthology more info
endosome	IEA Inferred from Electronic Annotation more info
extrinsic to endosome membrane	IDA Inferred from Direct Assay more info	PubMed
extrinsic to plasma membrane	ISO Inferred from Sequence Orthology more info
intracellular	IDA Inferred from Direct Assay more info	PubMed
intracellular	IPI Inferred from Physical Interaction more info	PubMed
membrane	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info
plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: ubiquitin carboxyl-terminal hydrolase 8

Names: ubiquitin carboxyl-terminal hydrolase 8; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; putative deubiquitinating enzyme; ubiquitin-specific-processing protease 8

NP_001239509.1

EC 3.4.19.12

NP_062703.2

EC 3.4.19.12

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252580.1 → NP_001239509.1 ubiquitin carboxyl-terminal hydrolase 8 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK129003, BY147904, BY639666

UniProtKB/TrEMBL

A2AI52

Conserved Domains (5) summary

cd02674
Location:833 – 1080
Blast Score: 694

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam08969
Location:6 – 124
Blast Score: 430

DUF1873; Domain of unknown function (DUF1873)

cl02553
Location:751 – 778
Blast Score: 133

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:749 – 1079
Blast Score: 818

UCH; Ubiquitin carboxyl-terminal hydrolase

cl00125
Location:210 – 321
Blast Score: 101

RHOD; Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins ...
NM_019729.3 → NP_062703.2 ubiquitin carboxyl-terminal hydrolase 8 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

BC050947, BY147904, BY639666

Consensus CDS

CCDS16687.1

UniProtKB/Swiss-Prot

Q80U87

Related

ENSMUSP00000028841, OTTMUSP00000016913, ENSMUST00000028841, OTTMUST00000037704

Conserved Domains (5) summary

cd02674
Location:822 – 1069
Blast Score: 697

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

pfam08969
Location:6 – 113
Blast Score: 439

DUF1873; Domain of unknown function (DUF1873)

cl02553
Location:740 – 767
Blast Score: 133

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:738 – 1068
Blast Score: 820

UCH; Ubiquitin carboxyl-terminal hydrolase

cl00125
Location:199 – 310
Blast Score: 101

RHOD; Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins ...

RefSeqs of Annotated Genomes: Build 38.1

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38 C57BL/6J

Genomic

NC_000068.7 Reference GRCm38 C57BL/6J

Range

126707328..126759314

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000024.1 Alternate Mm_Celera

Range

127946923..127999169

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AL732330.14	CAM14543.1
		CAM14544.1
genomic	CH466519.1	EDL28163.1	mixed
		EDL28164.1	mixed
		EDL28165.1	mixed
mRNA	AB045709.1	BAB18534.1
mRNA	AF057146.1	AAD38869.1
mRNA	AK040791.1	None	C57BL/6J
mRNA	AK122195.1	BAC65477.1
mRNA	AK129003.1	None
mRNA	AK134058.1	BAE21996.1	C57BL/6J
mRNA	AK142881.1	BAE25218.1	C57BL/6J
mRNA	AK153015.1	None	C57BL/6J
mRNA	AK163469.1	BAE37355.1	C57BL/6J
mRNA	AK166540.1	None	C57BL/6J
mRNA	AK204074.1	None
mRNA	BC027052.1	AAH27052.1	FVB/N
mRNA	BC038123.1	None	CZECHII
mRNA	BC050947.1	AAH50947.1	C57BL/6
mRNA	BC061465.1	AAH61465.1	C57BL/6
mRNA	BC066126.1	AAH66126.1
mRNA	BC092078.1	AAH92078.1	FVB/N
mRNA	BY147904.1	None	C57BL/6J
mRNA	BY639666.1	None	C57BL/6J

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q3TQM7	GenPept	UniProtKB/TrEMBL:Q3TQM7
Q3UQ26	GenPept	UniProtKB/TrEMBL:Q3UQ26
Q3UZ62	GenPept	UniProtKB/TrEMBL:Q3UZ62
Q58E42	GenPept	UniProtKB/TrEMBL:Q58E42
Q80U87.2	GenPept	UniProtKB/Swiss-Prot:Q80U87