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    MED25 mediator complex subunit 25 [ Homo sapiens ]

    Gene ID: 81857, updated on 19-May-2012
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    Summary

    Official Symbol
    MED25provided by HGNC
    Official Full Name
    mediator complex subunit 25provided by HGNC
    Primary source
    HGNC:28845
    Locus tag
    TCBAP0758
    See related
    Ensembl:ENSG00000104973; HPRD:14380; MIM:610197
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    P78; ACID1; ARC92; PTOV2; CMT2B2; TCBAP0758; MGC70671; DKFZp434K0512
    Summary
    This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]
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    Genomic context

    Location :
    19q13.3
    Sequence :
    Chromosome: 19; NC_000019.9 (50321536..50340237)
    See MED25 in Epigenomics, MapViewer

    Chromosome 19 - NC_000019.9Genomic Context describing neighboring genes Neighboring gene testis-specific serine kinase substrate Neighboring gene adaptor-related protein complex 2, alpha 1 subunit Neighboring gene fuzzy homolog (Drosophila) Neighboring gene uncharacterized LOC100506033 Neighboring gene prostate tumor overexpressed 1 Neighboring gene microRNA 4749

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes. Blomberg J, et al. Nucleic Acids Res, 2012 Mar 24. PMID 22447446.
    2. Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. Lee KA, et al. J Biol Chem, 2011 Dec 2. PMID 21987572.
    3. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
    4. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, et al. Mol Cell Proteomics, 2011 Oct. PMID 21890473.
    5. Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25. Eletsky A, et al. J Struct Funct Genomics, 2011 Sep. PMID 21785987.

    See all (33) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. solution NMR structure of protein MED25(391-543), comprising the activator interacting domain of subunit 25 of mediator, is presented with the measurement of polypeptide backbone heteronuclear 15N-{1H} NOEs to identify fast internal motional modes
      Title: Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.
    2. The regulatory interaction between Sox9, Wwp2 and Med25 defines the Sox9 transcriptional mechanisms of chondrogenesis in the forming palate.
      Title: Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25.
    3. Preliminary NMR chemical shift mapping showed that VP16 H2 (VP16C) interacts with MED25 ACID through one face of the beta-barrel, defined by strands B4-B7-B6.
      Title: NMR structure of the human Mediator MED25 ACID domain.
    4. report the NMR structure of the Mediator subunit Med25 (also called Arc92) activator interaction domain (ACID) and analyze the structural and functional interaction of ACID with the archetypical acidic transcription activator VP16
      Title: Structure and VP16 binding of the Mediator Med25 activator interaction domain.
    5. A hydrophobic furrow, formed by a beta-barrel and two alpha-helices in MED25 VBD, interacts tightly with VP16 TADn
      Title: Structure of the VP16 transactivator target in the Mediator.
    6. MED25 and PTOV1 differentially modulate retinoic acid sensitivity in cancer cells depending on their expression levels.
      Title: PTOV1 antagonizes MED25 in RAR transcriptional activation.
    7. These results define a role for Med25 and the Mediator complex in the regulation of xenobiotic metabolism and lipid homeostasis.
      Title: Med25 is required for RNA polymerase II recruitment to specific promoters, thus regulating xenobiotic and lipid metabolism in human liver.
    8. Lana-1-induced serum response element activation was inhibited by the dominant-negative N-terminal domain of the human MED25 mediator subunit, suggesting that this subunit mediates Lana-1-induced serum response element activation.
      Title: Kaposi's sarcoma-associated herpesvirus Lana-1 is a major activator of the serum response element and mitogen-activated protein kinase pathways via interactions with the Mediator complex.
    9. Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
      Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.
    10. data suggest that MED25, in cooperation with CBP and Mediators through its distinct domains, imposes a selective advantage on RAR/RXR activation
      Title: MED25 is distinct from TRAP220/MED1 in cooperating with CBP for retinoid receptor activation.
    Submit: New GeneRIF Correction See all (12)
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    Phenotypes

    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Charcot-Marie-Tooth disease, axonal, type 2B2

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    Mediator of RNA polymerase II transcription subunit 25 homolog P49336 CDK8    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog P51610 HCFC1    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog Q15648 MED1    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog Q96RN5 MED15    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog O75586 MED6    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog Q96G25 MED8    HPRD  PubMed  
    Mediator of RNA polymerase II transcription subunit 25 homolog MED9 MED9    HPRD  PubMed  
    NP_112235.2 NC_000021.7 TFF1    BIND  PubMed MED25 interacts with pS2 promoter. 
    BioGRID:123607 BioGRID:107458 CDK8    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:107777 CREBBP    BioGRID  PubMed Affinity Capture-Western; Co-localization; Two-hybrid 
    BioGRID:123607 BioGRID:114597 CTDP1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:123607 BioGRID:15703 DREB2A    BioGRID  PubMed Reconstituted Complex 
    BioGRID:123607 BioGRID:108403 ESR1    BioGRID  PubMed Two-hybrid 
    BioGRID:123607 BioGRID:111465 MED1    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western; Co-localization 
    BioGRID:123607 BioGRID:123974 MED10    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:134637 MED11    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:123607 BioGRID:115293 MED12    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:115294 MED13    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:114699 MED14    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:119623 MED15    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:115342 MED16    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:114830 MED17    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:120156 MED18    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:123607 BioGRID:128553 MED19    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:112704 MED22    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:123607 BioGRID:114829 MED23    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:123607 MED25    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:114831 MED26    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:123219 MED28    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:120734 MED29    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:118849 MED4    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:123607 BioGRID:115319 MED6    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:114833 MED7    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:123607 BioGRID:125219 MED8    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:123607 BioGRID:120403 MED9    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:109165 NR3C1    BioGRID  PubMed Two-hybrid 
    BioGRID:123607 BioGRID:123054 OBFC1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:114834 QKI    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:111849 RARA    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:123607 BioGRID:112168 RXRA    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:123607 BioGRID:112598 SREBF1    BioGRID  PubMed Affinity Capture-MS; Reconstituted Complex 
    BioGRID:123607 BioGRID:112734 TADA2A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:112923 THRA    BioGRID  PubMed Two-hybrid 
    BioGRID:123607 BioGRID:114735 TRIP4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:113900 TRRAP    BioGRID  PubMed Co-purification 
    BioGRID:123607 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:123607 BioGRID:971443 UL48    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:123607 BioGRID:116719 ZC3H13    BioGRID  PubMed Affinity Capture-MS 
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    General gene information

    Markers

    Genotypes

    Homology

    Pathways from BioSystems

    • Developmental Biology, organism-specific biosystem (from REACTOME)
      Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
    • Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem (from REACTOME)
      Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystemThe reactions involved in the metabolism of fatty acids and of the triacylglycerols and ketone bodies derived from them form a closely interrelated, coordinately regulated module that plays a central...
    • Gene Expression, organism-specific biosystem (from REACTOME)
      Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
    • Generic Transcription Pathway, organism-specific biosystem (from REACTOME)
      Generic Transcription Pathway, organism-specific biosystemOVERVIEW OF TRANSCRIPTION REGULATION: Detailed studies of gene transcription regulation in a wide variety of eukaryotic systems has revealed the general principles and mechanisms by which cell- or t...
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
      Metabolism of lipids and lipoproteins, organism-specific biosystemLipids are hydrophobic but otherwise chemically diverse molecules that play a wide variety of roles in human biology. They include ketone bodies, fatty acids, triacylglycerols, phospholipids and sphi...
    • PPARA Activates Gene Expression, organism-specific biosystem (from REACTOME)
      PPARA Activates Gene Expression, organism-specific biosystemThe set of genes regulated by PPAR-alpha is not fully known in humans, however many examples have been found in mice. Genes directly activated by PPAR-alpha contain peroxisome proliferator receptor e...
    • Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem (from REACTOME)
      Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystemPeroxisome proliferator-activated receptor alpha (PPAR-alpha) is the major regulator of fatty acid oxidation in the liver. PPARalpha is also the target of fibrate drugs used to treat abnormal plasma ...
    • Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem (from REACTOME)
      Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystemAdipogenesis is the process of cell differentiation by which preadipocytes become adipocytes. During this process the preadipocytes cease to proliferate, begin to accumulate lipid droplets and develo...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    retinoic acid receptor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    retinoid X receptor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    transcription factor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    gene expression TAS
    Traceable Author Statement
    more info
    		  
    negative regulation of fibroblast proliferation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    negative regulation of transcription from RNA polymerase II promoter IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    positive regulation of cell cycle arrest IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    positive regulation of chromatin binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    positive regulation of mediator complex assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    positive regulation of transcription from RNA polymerase II promoter IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    regulation of transcription, DNA-dependent IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Preferred Names
    mediator of RNA polymerase II transcription subunit 25
    Names
    mediator of RNA polymerase II transcription subunit 25
    prostate-derived protein 78 kDa
    activator-recruited cofactor 92 kDa component
    ARC/mediator transcriptional coactivator subunit
    activator interaction domain-containing protein 1
    mediator of RNA polymerase II transcription, subunit 25 homolog
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_017091.1 RefSeqGene

      Range
      5001..23702
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_030973.3 → NP_112235.2  mediator of RNA polymerase II transcription subunit 25

      Status: REVIEWED

      Source sequence(s)
      BC065297, BG770032, DC410997
      Consensus CDS
      CCDS33075.1
      UniProtKB/Swiss-Prot
      Q71SY5
      Related
      ENSP00000326767, ENST00000312865
      Conserved Domains (3) summary
      pfam11235
      Location:234 – 385
      Blast Score: 156
      Med25_SD1; Mediator complex subunit 25 synapsin 1
      pfam11232
      Location:392 – 545
      Blast Score: 712
      Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
      pfam11265
      Location:1 – 220
      Blast Score: 850
      Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000019.9 Reference GRCh37.p5 Primary Assembly

      Range
      50321536..50340237
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000151.1 Alternate HuRef

      Range
      46697846..46716581
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC006942.1 AAD15565.1
    genomic AC018766.2 (2597..12483) None
    genomic CH471177.1 EAW52544.1
      EAW52545.1
      EAW52546.1
      EAW52547.1
      EAW52548.1
      EAW52549.1
      EAW52550.1
      EAW52551.1
    mRNA AF261072.1 AAM20739.1
    mRNA AF283769.2 AAG15589.1
    mRNA AF447873.1 AAQ04648.1
    mRNA AJ617479.1 CAE84581.1
    mRNA AK055844.1 BAG51583.1
    mRNA AK289460.1 BAF82149.1
    mRNA AK298897.1 BAG61008.1
    mRNA AK307330.1 None
    mRNA AL136746.1 CAB66680.1
    mRNA AY533507.1 AAS45401.1
    mRNA BC021543.1 None
    mRNA BC024312.2 AAH24312.2
    mRNA BC065297.1 AAH65297.1
    mRNA BG770032.1 None
    mRNA BI520216.1 None
    mRNA DC410997.1 None
    mRNA EU392499.1 ACB88861.1
    mRNA EU392500.1 ACB88862.1
    mRNA EU392501.1 ACB88863.1
    mRNA EU392502.1 ACB88864.1
    mRNA EU392503.1 ACB88865.1
    mRNA EU392504.1 ACB88866.1
    mRNA EU392505.1 ACB88867.1
    mRNA EU392506.1 ACB88868.1
    mRNA EU392507.1 ACB88869.1
    mRNA EU392508.1 ACB88870.1
    mRNA EU392509.1 ACB88871.1
    mRNA EU392510.1 ACB88872.1
    mRNA EU392511.1 ACB88873.1
    mRNA EU392512.1 ACB88874.1
    mRNA EU392513.1 ACB88875.1
    mRNA EU392514.1 ACB88876.1
    mRNA EU392515.1 ACB88877.1
    mRNA EU392516.1 ACB88878.1
    mRNA EU392517.1 ACB88879.1
    mRNA EU392518.1 ACB88880.1
    mRNA EU392519.1 None
    other-genetic EU832505.1 ACE87677.1
    other-genetic EU832586.1 ACE86988.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q71MG4 GenPept UniProtKB/TrEMBL:Q71MG4
    Q71SY5.2 GenPept UniProtKB/Swiss-Prot:Q71SY5
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    Additional Links

    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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