TAF12 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa [Homo sapiens (human)] - Gene

Summary

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Official Symbol: TAF12provided by HGNC
Official Full Name: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDaprovided by HGNC
Primary source: HGNC:11545
See related: Ensembl:ENSG00000120656; HPRD:15981; MIM:600773; Vega:OTTHUMG00000003655
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TAF2J; TAFII20
Summary: Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]

Genomic context

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Location :: 1p35.3

Sequence :: Chromosome: 1; NC_000001.10 (28929608..28969604, complement)

See TAF12 in Epigenomics, MapViewer

Chromosome 1 - NC_000001.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that DNA binding by TAF4/4b-TAF12 facilitates the association of TFIID with the core promoter of a subset of genes.
Title: TAF4/4b x TAF12 displays a unique mode of DNA binding and is required for core promoter function of a subset of genes.
Data present the crystal structure of a complex formed by the interacting domains from two subunits of the general transcription factor TFIID, the human TATA binding protein-associated factors hTAF4 (hTAF(II)135) and hTAF12 (hTAF(II)20).
Title: Crystal structure of a subcomplex of human transcription factor TFIID formed by TATA binding protein-associated factors hTAF4 (hTAF(II)135) and hTAF12 (hTAF(II)20).
histone fold domain mediated interaction enhances the DNA binding activity of each of the TAF6-TAF9 and TAF4b-TAF12 pairs and of a histone-like octamer complex composed of the four TAFs
Title: Core promoter binding by histone-like TAF complexes.
TAF12 hitherto regarded as RNA polymerase II (RNAP II)-specific was found to be also involved in RNAP I transcription.
Title: Identification of novel functional TBP-binding sites and general factor repertoires.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Tat, p14	tat	Interaction of TFIID with the HIV-1 LTR, and therefore presumably HIV-1 Tat protein, is primarily dependent on the LTR TATA element and may also be stabilized or regulated by flanking E box motifs and basic helix-loop-helix proteins such as AP-4 and E47	PubMed
	tat	TATA binding protein (TBP)-associated factors (TAFs) in the TFIID complex are required for activation by transcription factors, but are dispensable for HIV-1 Tat LTR transactivation function	PubMed
	tat	A novel function of LBP-1, restricting HIV-1 transcription at the level of elongation by preventing the binding of TFIID to the promoter, is suppressed by HIV-1 Tat, indicating Tat helps recruit TFIID to the HIV-1 LTR	PubMed
	tat	HIV-1 Tat stabilizes the interaction of TFIIA with TFIID, and TFIIA and TFIID are required to reconstitute Tat-specific and TAR-dependent activation of HIV transcription in vitro	PubMed
	tat	HIV-1 Tat binds, through amino acids 36-50, directly to the TBP subunit of the TFIID holoenzyme complex (which includes at least TFIID subunits p250, p125, p70, TBP, and p30), and increases the interaction of TFIID with the HIV-1 LTR promoter	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_005635.1	NP_006847.1	ATF7	BIND	PubMed	TAF12 (hsTAF12) interacts ATF7.
NP_005635.1	Q92750	TAF4B	BIND	PubMed	TAF4B (hTAF4b) interacts with TAF12 (hTAF12).
Q16514	P17544	ATF7	HPRD	PubMed
Q16514	Q10570	CPSF1	HPRD	PubMed
Q16514	P21675	TAF1	HPRD	PubMed
Q16514	Q12962	TAF10	HPRD	PubMed
Q16514	Q15544	TAF11	HPRD	PubMed
Q16514	Q92804	TAF15	HPRD	PubMed
Q16514	Q6P1X5	TAF2	HPRD	PubMed
Q16514	O00268	TAF4	HPRD	PubMed
Q16514	Q15542	TAF5	HPRD	PubMed
Q16514	P49848	TAF6	HPRD	PubMed
Q16514	Q15545	TAF7	HPRD	PubMed
Q16514	Taube nuss	TAF8	HPRD	PubMed
Q16514	Q16594	TAF9	HPRD	PubMed
Q16514	P20226	TBP	HPRD	PubMed
BioGRID:112746	BioGRID:116206	ATF7	BioGRID	PubMed	Affinity Capture-Western; Co-localization; Reconstituted Complex
BioGRID:112746	BioGRID:112221	ATXN7	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112746	BioGRID:118946	CPSF1	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:108309	ELAVL1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:112746	BioGRID:108379	ERCC1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112746	BioGRID:113895	HIST3H3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112746	BioGRID:114375	KAT2B	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:112746	BioGRID:110694	MYC	BioGRID	PubMed	Co-purification
BioGRID:112746	BioGRID:110866	NFYA	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:110867	NFYB	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:110868	NFYC	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:114042	SUPT3H	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:112746	BioGRID:112744	TAF10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112746	BioGRID:114484	TAF1A	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:114482	TAF1C	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:112746	BioGRID:123775	TAF3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112746	BioGRID:112737	TAF4	BioGRID	PubMed	Co-crystal Structure; Far Western; Reconstituted Complex
BioGRID:112746	BioGRID:112738	TAF4B	BioGRID	PubMed	Reconstituted Complex
BioGRID:112746	BioGRID:112740	TAF5	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112746	BioGRID:112741	TAF6	BioGRID	PubMed	Two-hybrid
BioGRID:112746	BioGRID:112743	TAF9	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:112746	BioGRID:112771	TBP	BioGRID	PubMed	Affinity Capture-Western; Co-purification; Reconstituted Complex

Pathways from BioSystems

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Basal transcription factors, organism-specific biosystem (from KEGG)
Basal transcription factors, organism-specific biosystem
Basal transcription factors
Basal transcription factors, conserved biosystem (from KEGG)
Basal transcription factors, conserved biosystem
Basal transcription factors
C-MYC pathway, organism-specific biosystem (from Pathway Interaction Database)
C-MYC pathway, organism-specific biosystem
C-MYC pathway
Disease, organism-specific biosystem (from REACTOME)
Disease, organism-specific biosystemBiological processes are captured in Reactome by identifying the molecules (DNA, RNA, protein, small molecules) involved in them and describing the details of their interactions. From this molecular ...
Eukaryotic Transcription Initiation, organism-specific biosystem (from WikiPathways)
Eukaryotic Transcription Initiation, organism-specific biosystemIn eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. RNA polymerase is able to bind to core promoters in the pre...
Gene Expression, organism-specific biosystem (from REACTOME)
Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
HIV Infection, organism-specific biosystem (from REACTOME)
HIV Infection, organism-specific biosystemThe global pandemic of Human Immunodeficiency Virus (HIV) infection has resulted in tens of millions of people infected by the virus and millions more affected. UNAIDS estimates around 40 million ...
HIV Life Cycle, organism-specific biosystem (from REACTOME)
HIV Life Cycle, organism-specific biosystemThe life cycle of HIV-1 is divided into early and late phases, shown schematically in the figure. In the early phase, an HIV-1 virion binds to receptors and co-receptors on the human host cell surfac...
HIV-1 Transcription Initiation, organism-specific biosystem (from REACTOME)
HIV-1 Transcription Initiation, organism-specific biosystemFormation of the open complex exposes the template strand to the catalytic center of the RNA polymerase II enzyme. This facilitates formation of the first phosphodiester bond, which marks transcript...
Late Phase of HIV Life Cycle, organism-specific biosystem (from REACTOME)
Late Phase of HIV Life Cycle, organism-specific biosystemThe late phase of the HIV-1 life cycle includes the regulated expression of the HIV gene products and the assembly of viral particles. The assembly of viral particles will be covered in a later relea...
RNA Polymerase II HIV-1 Promoter Escape, organism-specific biosystem (from REACTOME)
RNA Polymerase II HIV-1 Promoter Escape, organism-specific biosystemRNA Polymerase II promoter escape occurs after the first phosphodiester bond has been created.
RNA Polymerase II Pre-transcription Events, organism-specific biosystem (from REACTOME)
RNA Polymerase II Pre-transcription Events, organism-specific biosystem
RNA Polymerase II Pre-transcription Events
RNA Polymerase II Promoter Escape, organism-specific biosystem (from REACTOME)
RNA Polymerase II Promoter Escape, organism-specific biosystemRNA Polymerase II promoter escape occurs after the first phosphodiester bond has been created.
RNA Polymerase II Transcription, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription, organism-specific biosystem
RNA Polymerase II Transcription
RNA Polymerase II Transcription Initiation, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation, organism-specific biosystemFormation of the open complex exposes the template strand to the catalytic center of the RNA polymerase II enzyme. This facilitates formation of the first phosphodiester bond, which marks transcript...
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Initiation And Promoter Clearance, organism-specific biosystem
RNA Polymerase II Transcription Initiation And Promoter Clearance
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystem (from REACTOME)
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening, organism-specific biosystemFormation of the pre-initiation complex
Transcription of the HIV genome, organism-specific biosystem (from REACTOME)
Transcription of the HIV genome, organism-specific biosystemExpression of the integrated HIV-1 provirus is dependent on the host cell Pol II transcription machinery, but is regulated in critical ways by HIV-1 Tat and Rev proteins. The long terminal repeats (L...
Validated targets of C-MYC transcriptional activation, organism-specific biosystem (from Pathway Interaction Database)
Validated targets of C-MYC transcriptional activation, organism-specific biosystem
Validated targets of C-MYC transcriptional activation

General gene information

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Markers

SHGC-30964 (e-PCR)
- UniSTS:2590

RH67949 (e-PCR)
- UniSTS:19683

Homology

Homologs of the TAF12 gene: The TAF12 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, chicken, zebrafish, fruit fly, mosquito, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
Map Viewer (Mouse)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to histone acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
contributes_to sequence-specific DNA binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
transcription factor binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA-dependent transcription, initiation	IDA Inferred from Direct Assay more info	PubMed
gene expression	TAS Traceable Author Statement more info
histone H3 acetylation	IDA Inferred from Direct Assay more info	PubMed
positive regulation of sequence-specific DNA binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
transcription elongation from RNA polymerase II promoter	TAS Traceable Author Statement more info
transcription from RNA polymerase II promoter	TAS Traceable Author Statement more info
transcription initiation from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
transcription initiation from RNA polymerase II promoter	TAS Traceable Author Statement more info
viral reproduction	TAS Traceable Author Statement more info
viral reproductive process	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
PCAF complex	IDA Inferred from Direct Assay more info	PubMed
STAGA complex	IDA Inferred from Direct Assay more info	PubMed
STAGA complex	IEA Inferred from Electronic Annotation more info
nucleoplasm	TAS Traceable Author Statement more info
transcription factor TFIID complex	IDA Inferred from Direct Assay more info	PubMed
transcription factor TFTC complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription initiation factor TFIID subunit 12

Names: transcription initiation factor TFIID subunit 12; TAFII20/TAFII15; TAFII-20/TAFII-15; transcription initiation factor TFIID 20/15 kDa subunits; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001135218.1 → NP_001128690.1 transcription initiation factor TFIID subunit 12

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AL840644, BC011986, BE787487, BM676557

Consensus CDS

CCDS326.1

UniProtKB/Swiss-Prot

Q16514

Related

ENSP00000263974, OTTHUMP00000003785, ENST00000263974, OTTHUMT00000010367

Conserved Domains (1) summary

cd07981
Location:57 – 128
Blast Score: 326

TAF12; TATA Binding Protein (TBP) Associated Factor 12 (TAF12) is one of several TAFs that bind TBP and is involved in forming Transcription Factor IID (TFIID) complex
NM_005644.3 → NP_005635.1 transcription initiation factor TFIID subunit 12

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.

Source sequence(s)

BM676557, BX095156, U57693

Consensus CDS

CCDS326.1

UniProtKB/Swiss-Prot

Q16514

Related

ENSP00000362930, OTTHUMP00000003786, ENST00000373824, OTTHUMT00000010368

Conserved Domains (1) summary

cd07981
Location:57 – 128
Blast Score: 326

TAF12; TATA Binding Protein (TBP) Associated Factor 12 (TAF12) is one of several TAFs that bind TBP and is involved in forming Transcription Factor IID (TFIID) complex

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000001.10 Reference GRCh37.p10 Primary Assembly

Range

28929608..28969604, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000133.1 Alternate HuRef

Range

27182618..27222688, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018912.1 Alternate CHM1_1.0

Range

29148881..29188946, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01035046.1 (11845..19288)	None
genomic	ABBA01035047.1	None
genomic	ABBA01035048.1	None
genomic	ABBA01035049.1	None
genomic	ABBA01035050.1	None
genomic	ABBA01035051.1	None
genomic	AL513497.21 (102592..135820)	None
genomic	AL840644.2 (2001..8748)	None
genomic	AMYH01000433.1 (59903..85031)	None
genomic	AMYH01000434.1	None
genomic	AY206865.1	AAO13491.1
genomic	CH471059.2	EAX07680.1
		EAX07681.1
		EAX07682.1
		EAX07683.1
mRNA	AK310097.1	None
mRNA	BC011986.1	AAH11986.1
mRNA	BE787487.1	None
mRNA	BM676557.1	None
mRNA	BT007031.1	AAP35678.1
mRNA	BX095156.1	None
mRNA	CX865268.1	None
mRNA	D50544.1	BAA09112.1
mRNA	U57693.1	AAC50600.1
		AAC50601.1
mRNA	X84002.1	CAA58826.1
other-genetic	DQ890580.2	ABM92197.1
other-genetic	DQ893739.2	ABM84665.1