TAF2 TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa [Homo sapiens] - Gene

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Summary

Official Symbol: TAF2provided by HGNC
Official Full Name: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDaprovided by HGNC
Primary source: HGNC:11536
See related: Ensembl:ENSG00000064313; HPRD:07060; MIM:604912; Vega:OTTHUMG00000165009
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TAF2B; CIF150; TAFII150
Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 8q24.12

Sequence :: Chromosome: 8; NC_000008.10 (120743014..120845074, complement)

See TAF2 in Epigenomics, MapViewer

Chromosome 8 - NC_000008.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Interaction of TFIID with the HIV-1 LTR, and therefore presumably HIV-1 Tat protein, is primarily dependent on the LTR TATA element and may also be stabilized or regulated by flanking E box motifs and basic helix-loop-helix proteins such as AP-4 and E47	PubMed
	tat	TATA binding protein (TBP)-associated factors (TAFs) in the TFIID complex are required for activation by transcription factors, but are dispensable for HIV-1 Tat LTR transactivation function	PubMed
	tat	A novel function of LBP-1, restricting HIV-1 transcription at the level of elongation by preventing the binding of TFIID to the promoter, is suppressed by HIV-1 Tat, indicating Tat helps recruit TFIID to the HIV-1 LTR	PubMed
	tat	HIV-1 Tat stabilizes the interaction of TFIIA with TFIID, and TFIIA and TFIID are required to reconstitute Tat-specific and TAR-dependent activation of HIV transcription in vitro	PubMed
	tat	HIV-1 Tat binds, through amino acids 36-50, directly to the TBP subunit of the TFIID holoenzyme complex (which includes at least TFIID subunits p250, p125, p70, TBP, and p30), and increases the interaction of TFIID with the HIV-1 LTR promoter	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_003175.1	NP_006702.1	RNPS1	BIND	PubMed	RNPS1 interacts with CIF150.
NP_003175.1	NP_003185.1	TBP	BIND	PubMed	TBP interacts with TAFII150. This interaction was modelled on a demonstrated interaction between human TBP and TAFII150 from Drosophila.
Q6P1X5	P21675	TAF1	HPRD	PubMed
Q6P1X5	Q12962	TAF10	HPRD	PubMed
Q6P1X5	Q15544	TAF11	HPRD	PubMed
Q6P1X5	Q16514	TAF12	HPRD	PubMed
Q6P1X5	O00268	TAF4	HPRD	PubMed
Q6P1X5	Q15542	TAF5	HPRD	PubMed
Q6P1X5	P49848	TAF6	HPRD	PubMed
Q6P1X5	Q15545	TAF7	HPRD	PubMed
Q6P1X5	Taube nuss	TAF8	HPRD	PubMed
Q6P1X5	Q16594	TAF9	HPRD	PubMed
BioGRID:112736	BioGRID:115984	KDM5B	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112736	BioGRID:112735	TAF1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:112736	BioGRID:112744	TAF10	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112736	BioGRID:112747	TAF13	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112736	BioGRID:126205	TAF8	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112736	BioGRID:112743	TAF9	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112736	BioGRID:112771	TBP	BioGRID	PubMed	Affinity Capture-Western; Co-purification; Reconstituted Complex
BioGRID:112736	BioGRID:112872	TERF1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112736	BioGRID:112873	TERF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:112736	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
metallopeptidase activity	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
transcription regulatory region DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
response to organic cyclic compound	IEA Inferred from Electronic Annotation more info
transcription initiation from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
nucleus	IEA Inferred from Electronic Annotation more info
transcription factor TFIID complex	IDA Inferred from Direct Assay more info	PubMed
NOT transcription factor TFIID complex	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with transcription factor TFIID complex	IDA Inferred from Direct Assay more info	PubMed
transcription factor TFTC complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: transcription initiation factor TFIID subunit 2

Names: transcription initiation factor TFIID subunit 2; TAFII-150; TAF(II)150; TBP-associated factor 150 kDa; 150 kDa cofactor of initiator function; cofactor of initiator function, 150kD subunit; RNA polymerase II TBP-associated factor subunit B; transcription initiation factor TFIID 150 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003184.3 → NP_003175.1 transcription initiation factor TFIID subunit 2

Status: REVIEWED

Source sequence(s)

AF040701, AK001618, BC008473, BC035673, BC047732, BC064830

Consensus CDS

CCDS34937.1

UniProtKB/TrEMBL

B3KMD8

UniProtKB/Swiss-Prot

Q6P1X5

Related

ENSP00000367406, OTTHUMP00000228190, ENST00000378164, OTTHUMT00000381436

Conserved Domains (1) summary

cd09839
Location:27 – 541
Blast Score: 2073

M1_TAF2; TATA binding protein (TBP) associated factor 2

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000008.10 Reference GRCh37.p5 Primary Assembly

Range

120743014..120845074, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000140.1 Alternate HuRef

Range

116063646..116165955, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC021945.14 (28105..101036)	None
genomic	AC107960.3 (151726..180853)	None
genomic	CH471060.1	EAW91991.1
		EAW91992.1
		EAW91993.1
mRNA	AF026445.1	AAC02966.1
mRNA	AF040701.1	AAC68502.1
mRNA	AF057694.1	AAC13540.1
mRNA	AK001618.1	BAG50950.1
mRNA	AK001909.1	BAG50991.1
mRNA	AK316592.1	BAG38179.1
mRNA	AL137601.1	CAB70836.1
mRNA	AU098502.1	None
mRNA	BC008473.1	None
mRNA	BC018010.1	None
mRNA	BC035673.2	AAH35673.1
mRNA	BC047732.1	AAH47732.1
mRNA	BC064830.1	AAH64830.1
other-genetic	BC146379.1	AAI46380.1