SPN sialophorin [Homo sapiens (human)] - Gene

Summary

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Official Symbol: SPNprovided by HGNC
Official Full Name: sialophorinprovided by HGNC
Primary source: HGNC:11249
See related: Ensembl:ENSG00000197471; HPRD:06770; MIM:182160
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: LSN; CD43; GALGP; GPL115
Summary: The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Genomic context

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Location :: 16p11.2

Sequence :: Chromosome: 16; NC_000016.9 (29674271..29681828)

See SPN in Epigenomics, MapViewer

Chromosome 16 - NC_000016.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that, despite the high CD43 expression in both tumorous and nontumorous Langerhans cells (LCs), the JL1 epitope of CD43 is exposed in immature and neoplastic LCs.
Title: Use of the JL1 epitope, which encompasses the nonglycosylation site of CD43, as a marker of immature/neoplastic Langerhans cells.
Compared to healthy controls, both CD43 mRNA and protein expressions were reduced in T cells from patients with SLE, and were inversely correlated with IgG.
Title: Abnormal expression of CD43 in patients with systemic lupus erythematosus and its clinical significance.
CD43 localizes to the nucleus, where it binds chromatin, co-localizes and co-immunoprecipitates with beta-catenin, and enhances the reporter gene expression regulated by beta-catenin
Title: Leukocyte marker CD43 promotes cell growth in co-operation with β-catenin in non-hematopoietic cancer cells.
The capping of CD43 on the cell surface is a strong signal for phagocytosis that allows phagocytes to differentiate between healthy and apoptotic cells without any additional membrane changes
Title: Clearance of CD43-capped cells by macrophages: capping alone leads to phagocytosis.
The anti-adhesive function of CD43 in colon carcinoma cells plays a role in the tumorigenesis and metastasis of colorectal carcinoma cells.
Title: Anti-adhesive functions of CD43 expressed on colon carcinoma cells through the modulation of integrins.
there exists a negative feedback loop between p53 and CD43: CD43-dependent signaling activates p53, which in turn downregulates the expression of CD43
Title: Tumor suppressor p53 down-regulates expression of human leukocyte marker CD43 in non-hematopoietic tumor cells.
O-glycosylated CD43 and CD45 molecules on T cells regulates cell adhesion and favors the transmission of HTLV-1 from cell to cell.
Title: Role of O-glycosylation and expression of CD43 and CD45 on the surfaces of effector T cells in human T cell leukemia virus type 1 cell-to-cell infection.
Data show that Pic, a class 2 SPATE protein produced by Shigella flexneri 2a targets a broad range of human leukocyte glycoproteins including CD43, CD44, CD45, CD93, CD162 and the surface-attached chemokine fractalkine.
Title: Serine protease autotransporters from Shigella flexneri and pathogenic Escherichia coli target a broad range of leukocyte glycoproteins.
identification of the tumor antigen UN1 as the transmembrane CD43 sialoglycoprotein
Title: Mass spectrometry-based identification of the tumor antigen UN1 as the transmembrane CD43 sialoglycoprotein.
Expression of CD43 induces cell rounding, inhibition of cell re-attachment, augmentation of microvilli, and phosphorylation of Ezrin/Radixin/Moesin (ERM)in HEK293T cells.
Title: Formation of microvilli and phosphorylation of ERM family proteins by CD43, a potent inhibitor for cell adhesion: cell detachment is a potential cue for ERM phosphorylation and organization of cell morphology.

Submit: New GeneRIF Correction See all (32)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Elimination of CD43 expression by gene targeting in the CEM T cell line results in its increased homotypic adhesion and binding to HIV-1 gp120	PubMed
Gag, Pr55	gag	HIV-1 Gag co-localizes with both uropod markers PSGL-1 and CD43 on the plasma membrane	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
P16150	P35222	CTNNB1	HPRD	PubMed
P16150	Q9UER7	DAXX	HPRD	PubMed
P16150	P15311	EZR	HPRD	PubMed
P16150	P06241	FYN	HPRD	PubMed
P16150	P05362	ICAM1	HPRD	PubMed
P16150	P09382	LGALS1	HPRD	PubMed
P16150	P26038	MSN	HPRD	PubMed
P16150	Q9BZZ2	SIGLEC1	HPRD	PubMed
BioGRID:112571	BioGRID:107985	DAXX	BioGRID	PubMed	Two-hybrid
BioGRID:112571	BioGRID:113271	EZR	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:112571	BioGRID:110147	LGALS1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:112571	BioGRID:110584	MSN	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex

Pathways from BioSystems

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Basigin interactions, organism-specific biosystem (from REACTOME)
Basigin interactions, organism-specific biosystemBasigin is a widely expressed transmembrane glycoprotein that belongs to the Ig superfamily and is highly enriched on the surface of epithelial cells. Basigin is involved in intercellular interaction...
Cell adhesion molecules (CAMs), organism-specific biosystem (from KEGG)
Cell adhesion molecules (CAMs), organism-specific biosystemCell adhesion molecules are (glyco)proteins expressed on the cell surface and play a critical role in a wide array of biologic processes that include hemostasis, the immune response, inflammation, em...
Cell adhesion molecules (CAMs), conserved biosystem (from KEGG)
Cell adhesion molecules (CAMs), conserved biosystemCell adhesion molecules are (glyco)proteins expressed on the cell surface and play a critical role in a wide array of biologic processes that include hemostasis, the immune response, inflammation, em...
Cell surface interactions at the vascular wall, organism-specific biosystem (from REACTOME)
Cell surface interactions at the vascular wall, organism-specific biosystemLeukocyte extravasation is a rigorously controlled process that guides white cell movement from the vascular lumen to sites of tissue inflammation. The powerful adhesive interactions that are require...
Epstein-Barr virus infection, organism-specific biosystem (from KEGG)
Epstein-Barr virus infection, organism-specific biosystemEpstein-Barr virus (EBV) is a ubiquitous human herpesvirus that is associated with oncogenesis. EBV infection to primary human B lymphocytes leads to induction of EBV-specific HLA-restricted cytotoxi...
Epstein-Barr virus infection, conserved biosystem (from KEGG)
Epstein-Barr virus infection, conserved biosystemEpstein-Barr virus (EBV) is a ubiquitous human herpesvirus that is associated with oncogenesis. EBV infection to primary human B lymphocytes leads to induction of EBV-specific HLA-restricted cytotoxi...
Hemostasis, organism-specific biosystem (from REACTOME)
Hemostasis, organism-specific biosystemHemostasis is a physiological response that culminates in the arrest of bleeding from an injured vessel. Under normal conditions the vascular endothelium supports vasodilation, inhibits platelet adhe...

General gene information

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Markers

SPN_423 (e-PCR)
- UniSTS:277767

D11S2921 (e-PCR)
- UniSTS:152074

GDB:196680 (e-PCR)
- UniSTS:66323

GDB:434012 (e-PCR)
- UniSTS:157204

D11S2560 (e-PCR)
- UniSTS:37928

D8S2279 (e-PCR)
- UniSTS:473907

RH93020 (e-PCR)
- UniSTS:93009

RH70827 (e-PCR)
- UniSTS:87890

D16S2670 (e-PCR)
- UniSTS:152419

D10S16 (e-PCR)
- UniSTS:155756

D15S1477 (e-PCR)
- UniSTS:474482

D1S1425 (e-PCR)
- UniSTS:149621

G35510 (e-PCR)
- UniSTS:44150

D7S511 (e-PCR)
- UniSTS:10880

G32839 (e-PCR)
- UniSTS:117418

GDB:626036 (e-PCR)
- UniSTS:158381

GDB:313783 (e-PCR)
- UniSTS:13512

GDB:631802 (e-PCR)
- UniSTS:158429

G15885 (e-PCR)
- UniSTS:32221

GDB:631813 (e-PCR)
- UniSTS:158430

RH67531 (e-PCR)
- UniSTS:86517

GDB:180921 (e-PCR)
- UniSTS:155126

Homology

Homologs of the SPN gene: The SPN gene is conserved in chimpanzee, cow, mouse, and rat.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
bacterial cell surface binding	IDA Inferred from Direct Assay more info	PubMed
transmembrane signaling receptor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
T cell costimulation	IEA Inferred from Electronic Annotation more info
blood coagulation	TAS Traceable Author Statement more info
cell surface receptor signaling pathway	IEA Inferred from Electronic Annotation more info
cellular defense response	TAS Traceable Author Statement more info	PubMed
chemotaxis	TAS Traceable Author Statement more info	PubMed
defense response to bacterium	IDA Inferred from Direct Assay more info	PubMed
establishment or maintenance of cell polarity	TAS Traceable Author Statement more info	PubMed
immune response	TAS Traceable Author Statement more info	PubMed
leukocyte migration	TAS Traceable Author Statement more info
negative regulation of T cell proliferation	IEA Inferred from Electronic Annotation more info
negative regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
negative regulation of type IV hypersensitivity	IEA Inferred from Electronic Annotation more info
negative thymic T cell selection	IEA Inferred from Electronic Annotation more info
positive regulation of T cell proliferation	IEA Inferred from Electronic Annotation more info
positive regulation of tumor necrosis factor biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
regulation of defense response to virus	IEA Inferred from Electronic Annotation more info
response to protozoan	IEA Inferred from Electronic Annotation more info
signal transduction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
basement membrane	IEA Inferred from Electronic Annotation more info
cell surface	IDA Inferred from Direct Assay more info
external side of plasma membrane	IEA Inferred from Electronic Annotation more info
extracellular space	IDA Inferred from Direct Assay more info	PubMed
integral to plasma membrane	IEA Inferred from Electronic Annotation more info
plasma membrane	TAS Traceable Author Statement more info
uropod	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: leukosialin

Names: leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001030288.2 → NP_001025459.1 leukosialin precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC009086, DA951208, X60502

Consensus CDS

CCDS10650.1

UniProtKB/TrEMBL

A8K9B1

UniProtKB/Swiss-Prot

P16150
NM_003123.4 → NP_003114.1 leukosialin precursor

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC009086, AK292626, DB108930

Consensus CDS

CCDS10650.1

UniProtKB/TrEMBL

A8K9B1

UniProtKB/Swiss-Prot

P16150

Related

ENSP00000353238, ENST00000360121

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000016.9 Reference GRCh37.p10 Primary Assembly

Range

29674271..29681828

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000148.1 Alternate HuRef

Range

27335753..27343116

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018927.1 Alternate CHM1_1.0

Range

30571236..30578789

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01013122.1 (20501..21354)	None
genomic	ABBA01013123.1	None
genomic	AC009086.6 (173102..180631)	None
genomic	AMYH01007494.1 (337947..345500)	None
genomic	CH471238.1	EAW80015.1
		EAW80016.1
genomic	M61827.1	AAA51949.1
genomic	X52075.1	CAA36294.1
mRNA	AK292626.1	BAF85315.1
mRNA	AK313750.1	BAG36490.1
mRNA	BC012350.1	AAH12350.1
mRNA	BC035510.1	None
mRNA	DA951208.1	None
mRNA	DB108930.1	None
mRNA	J04536.1	AAB59540.1
mRNA	X60502.1	None
other-genetic	DQ892262.2	ABM83188.1
other-genetic	DQ895464.2	ABM86390.1