ARAP3 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 [Homo sapiens] - Gene

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Summary

Official Symbol: ARAP3provided by HGNC
Official Full Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3provided by HGNC
Primary source: HGNC:24097
See related: Ensembl:ENSG00000120318; HPRD:09441; MIM:606647; Vega:OTTHUMG00000129610
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DRAG1; CENTD3; FLJ21065
Summary: This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2008]

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Genomic context

Location :: 5q31.3

Sequence :: Chromosome: 5; NC_000005.9 (141032968..141061800, complement)

See ARAP3 in Epigenomics, MapViewer

Chromosome 5 - NC_000005.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ARAP3 is a unique Src substrate that suppresses peritoneal dissemination of scirrhous gastric carcinoma cells.
Title: ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion and invasion.
None of the individual PH domains can bind to PtdIns(3,4,5)P3; rather, a fragment comprising two PH domains and an N-terminal linker is minimally required for binding.
Title: ARAP3 binding to phosphatidylinositol-(3,4,5)-trisphosphate depends on N-terminal tandem PH domains and adjacent sequences.
ARAP3 deficiency produced by antisense expression of an ARAP3 EST impaired entry of anthrax PA and its bound toxigenic moieties into both human and mouse cells, resulting in reduced toxin sensitivity
Title: EST-based genome-wide gene inactivation identifies ARAP3 as a host protein affecting cellular susceptibility to anthrax toxin.
The catalytic domain of ARF-GAP alone is sufficient to initiate uncoating of liposome-derived COPI-coated vesicles. ARF-GAP activity is not required for COPI coat assembly & does not seem to be an essential coat component of COPI vesicles.
Title: Functional reconstitution of COPI coat assembly and disassembly using chemically defined components.
ARAP3 has a role in integrin-mediated tyrosine kinase signalling pathways controlling Rho GTPases and cell spreading
Title: ARAP3 is transiently tyrosine phosphorylated in cells attaching to fibronectin and inhibits cell spreading in a RhoGAP-dependent manner.
In a yeast two-hybrid screen for new interaction partners of Arap3, the PI 5'-phosphatase SHIP2 was identified as an interaction partner of Arap3.
Title: The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description
BioGRID:122163	BioGRID:113164	UBC		BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ARF GTPase activator activity	ISS Inferred from Sequence or Structural Similarity more info
NOT Rac GTPase activator activity	ISS Inferred from Sequence or Structural Similarity more info
Rho GTPase activator activity	IEA Inferred from Electronic Annotation more info
Rho GTPase activator activity	ISS Inferred from Sequence or Structural Similarity more info
metal ion binding	IEA Inferred from Electronic Annotation more info
phosphatidylinositol-3,4,5-trisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
phosphatidylinositol-3,4-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
negative regulation of Rac protein signal transduction	IEA Inferred from Electronic Annotation more info
negative regulation of Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
negative regulation of Rho protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info
negative regulation of cell migration	IEA Inferred from Electronic Annotation more info
negative regulation of cell migration	ISS Inferred from Sequence or Structural Similarity more info
positive regulation of Rho GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
regulation of ARF GTPase activity	IEA Inferred from Electronic Annotation more info
regulation of cell shape	IEA Inferred from Electronic Annotation more info
regulation of cell shape	ISS Inferred from Sequence or Structural Similarity more info
regulation of small GTPase mediated signal transduction	TAS Traceable Author Statement more info
signal transduction	IEA Inferred from Electronic Annotation more info
small GTPase mediated signal transduction	TAS Traceable Author Statement more info
vesicle-mediated transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IC Inferred by Curator more info	PubMed
cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
cytoskeleton	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
intracellular	IEA Inferred from Electronic Annotation more info
lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
plasma membrane	IEA Inferred from Electronic Annotation more info
ruffle	ISS Inferred from Sequence or Structural Similarity more info

General protein information

Preferred Names: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Names: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3; cnt-d3; centaurin-delta-3; centaurin, delta 3; PtdIns(3,4,5)P3-binding protein; phosphoinositide binding protein; Arf and Rho GAP adapter protein 3; ARF-GAP, RHO-GAP, ankyrin repeat and plekstrin homology domains-containing protein 3

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022481.5 → NP_071926.4 arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Status: REVIEWED

Source sequence(s)

AJ310567, AK024718, BC025757, BX489939

Consensus CDS

CCDS4266.1

UniProtKB/TrEMBL

Q05CH1

UniProtKB/Swiss-Prot

Q8WWN8

UniProtKB/TrEMBL

Q9H7C1

Related

ENSP00000239440, OTTHUMP00000160010, ENST00000239440, OTTHUMT00000251805

Conserved Domains (6) summary

cd04385
Location:909 – 1084
Blast Score: 703

RhoGAP_ARAP; RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ...

cd00821
Location:291 – 377
Blast Score: 162

PH; Pleckstrin homology (PH) domain

cd01768
Location:1120 – 1207
Blast Score: 106

RA; RA (Ras-associating) ubiquitin domain

cd09490
Location:4 – 66
Blast Score: 199

SAM_Arap1,2,3; SAM domain of Arap1,2,3 (angiotensin receptor-associated protein)

cl00273
Location:399 – 482
Blast Score: 111

PH-like; Pleckstrin homology-like domain

cl02464
Location:490 – 606
Blast Score: 377

ArfGap; Putative GTPase activating protein for Arf

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000005.9 Reference GRCh37.p5 Primary Assembly

Range

141032968..141061800, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000137.1 Alternate HuRef

Range

136177475..136206231, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC022420.6 (49728..78560)	None
genomic	CH471062.2	EAW61904.1
		EAW61905.1
		EAW61906.1
		EAW61907.1
mRNA	AJ310567.1	CAC83946.1
mRNA	AK001579.1	BAG50942.1
mRNA	AK024718.1	BAB14973.1
mRNA	AK091385.1	None
mRNA	AK290052.1	BAF82741.1
mRNA	AK295768.1	BAG58593.1
mRNA	BC009968.2	AAH09968.3
mRNA	BC025757.1	AAH25757.1
mRNA	BC027880.1	AAH27880.1
mRNA	BX489939.1	None

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q05CA7	GenPept	UniProtKB/TrEMBL:Q05CA7
Q05CH1	GenPept	UniProtKB/TrEMBL:Q05CH1
Q8WWN8.1	GenPept	UniProtKB/Swiss-Prot:Q8WWN8
Q96G49	GenPept	UniProtKB/TrEMBL:Q96G49
Q9H7C1	GenPept	UniProtKB/TrEMBL:Q9H7C1