ABCE1 ATP-binding cassette, sub-family E (OABP), member 1 [Homo sapiens (human)] - Gene

Summary

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Official Symbol: ABCE1provided by HGNC
Official Full Name: ATP-binding cassette, sub-family E (OABP), member 1provided by HGNC
Primary source: HGNC:69
Locus tag: OK/SW-cl.40
See related: Ensembl:ENSG00000164163; HPRD:03132; MIM:601213; Vega:OTTHUMG00000161478
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RLI; OABP; ABC38; RNS4I; RNASEL1; RNASELI
Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Genomic context

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Location :: 4q31

Sequence :: Chromosome: 4; NC_000004.11 (146019156..146050676)

See ABCE1 in Epigenomics, MapViewer

Chromosome 4 - NC_000004.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABCE1 protein regulates a broad range of biological functions including viral infection, tumor cell proliferation, and antiapoptosis. (Review)
Title: The biological regulation of ABCE1.
For binding of WT HIV-1 Gag to the cellular ATPase ABCE1, which facilitates HIV-1 capsid assembly, the basic residues in the nucleocapsid domain of Gag are required but the cysteine and histidine residues in the nucleocapsid domain are dispensable.
Title: Basic residues in the nucleocapsid domain of Gag are required for interaction of HIV-1 gag with ABCE1 (HP68), a cellular protein important for HIV-1 capsid assembly.
Pulse chase experiments and immunoelectron microscopy shows that HIV-1 Gag associates transiently with ABCE1 during HIV-1 capsid assembly, including at the plasma membrane where assembly is completed.
Title: Host ABCE1 is at plasma membrane HIV assembly sites and its dissociation from Gag is linked to subsequent events of virus production.
HIV-1, HIV-2, SIV mac239, and SIVagm Gag proteins form ABCE1-containing assembly intermediates during immature capsid formation, indicating that Gag proteins of these diverse retroviruses bind to ABCE1 despite the limited homology between these Gags.
Title: Conservation of a stepwise, energy-sensitive pathway involving HP68 for assembly of primate lentivirus capsids in cells.
During assembly of an immature HIV-1 capsid, HIV-1 Gag progresses through a pathway of sequential assembly intermediates that contain ABCE1, a cellular ATPase that was found to facilitate capsid formation.
Title: Identification of a host protein essential for assembly of immature HIV-1 capsids.
To form HIV-1 capsid assembly intermediates, HIV-1 Gag co-opts a complex found in infected and uninfected cells that contains the cellular ATPase ABCE1 and the RNA helicase DDX6, both of which facilitate HIV-1 capsid assembly.
Title: HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.
Report high expression level of ABCE1 mRNA and protein in human lung adenocarcinoma tissues and metastatic lymph nodes, which was also correlated with clinical stages.
Title: Role of the ABCE1 gene in human lung adenocarcinoma.
Pelota/Hbs1 induced dissociation of elongation complexes from ribosomes and release of peptidyl-tRNA, but only in the presence of ABCE1.
Title: Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes.
Results suggest that ABCE1 plays an important role in the pathogenesis of human small cell lung cancer cell.
Title: A small interfering ABCE1-targeting RNA inhibits the proliferation and invasiveness of small cell lung cancer.
NTP hydrolysis by ABCE1 is stimulated by posttermination complexes and is required for its ribosomal recycling activity.
Title: The role of ABCE1 in eukaryotic posttermination ribosomal recycling.

Submit: New GeneRIF Correction See all (18)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Gag, Pr55	gag	HIV-1 GagZip chimeric proteins, in which NC was replaced with a heterologous leucine zipper, form ABCE1-containing intermediates, suggesting exposure of an ABCE1-binding domain located elsewhere in Gag, outside NC	PubMed
	gag	ABCE1, DDX6, and AGO2 proteins are associated with HIV-1 Gag in the intracellular high-molecular-weight assembly intermediates	PubMed
	gag	The nucleocapsid (NC) domain of Gag is critical for interaction with endogenous ABCE1 in primate cells; basic residues in NC are critical for the Gag-ABCE1 interaction, while the cysteine and histidine residues in the zinc fingers are dispensable	PubMed
	gag	ABCE1 co-localizes with Gag clusters present at the plasma membrane	PubMed
	gag	Cells expressing a protease-deficient HIV-1 molecular clone demonstrate a prolonged association of Gag and ABCE1	PubMed
	gag	Expression of TULA proteins reduces the production of HIV-1 particles and modulates Gag processing. ABCE1 recruits TULA to Gag	PubMed
	gag	Gag production is significantly increased by fusing HIV-1-infected CD4- and CXCR4-expressing rat fibroblasts with uninfected human T-, B-, and macrophage cell lines. This process is regulated by HP68 and MHC class II transactivator (CIITA)	PubMed
	gag	HP68 associates post-translationally with HIV-1 Gag and Vif, an interaction that requires the Nucleocapsid domain of Gag and that is essential for assembly of immature HIV-1 capsids	PubMed
Vif, p23	vif	HuHP68 is essential for HIV-1 capsid assembly and associates with HIV-1 Vif in the form of an HuHP68 complex with HIV-1 Gag	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
P61221	P10153	RNASE2	HPRD	PubMed
P61221	Q05823	RNASEL	HPRD	PubMed
BioGRID:111986	BioGRID:106671	AP1B1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:114030	CUL3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:121157	DIABLO	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:114214	EIF3F	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:114216	EIF3H	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:109883	ITGA4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:122103	MMS19	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:109180	PDIA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:111969	RNASEL	BioGRID	PubMed	Reconstituted Complex
BioGRID:111986	BioGRID:119602	SIRT7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:119748	UBASH3A	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:111986	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:131884	USP50	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111986	BioGRID:1205537	gag	BioGRID	PubMed	Affinity Capture-Western

General gene information

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Markers

D8S2278 (e-PCR)
- UniSTS:473906

ABCE1 (e-PCR)
- UniSTS:503368

D1S1423 (e-PCR)
- UniSTS:149619

GDB:451571 (e-PCR)
- UniSTS:157314

RH70908 (e-PCR)
- UniSTS:71080

RH104489 (e-PCR)
- UniSTS:98801

SHGC-50255 (e-PCR)
- UniSTS:82170

Homology

Homologs of the ABCE1 gene: The ABCE1 gene is conserved in Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
ATPase activity	IEA Inferred from Electronic Annotation more info
electron carrier activity	IEA Inferred from Electronic Annotation more info
iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
ribonuclease inhibitor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
RNA catabolic process	TAS Traceable Author Statement more info	PubMed
response to virus	TAS Traceable Author Statement more info	PubMed
virus-host interaction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ATP-binding cassette sub-family E member 1

Names: ATP-binding cassette sub-family E member 1; huHP68; RNase L inhibitor; ribonuclease 4 inhibitor; 2'-5'-oligoadenylate-binding protein; ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001040876.1 → NP_001035809.1 ATP-binding cassette sub-family E member 1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC096757, BC016283, DA218189

Consensus CDS

CCDS34071.1

UniProtKB/Swiss-Prot

P61221

Conserved Domains (4) summary

COG1245
Location:4 – 597
Blast Score: 2116

COG1245; Predicted ATPase, RNase L inhibitor (RLI) homolog [General function prediction only]

pfam04068
Location:6 – 37
Blast Score: 128

RLI; Possible Fer4-like domain in RNase L inhibitor, RLI

cd03236
Location:78 – 332
Blast Score: 1214

ABC_RNaseL_inhibitor_domain1; The ATP-binding cassette domain 1 of RNase L inhibitor.

cd03237
Location:359 – 592
Blast Score: 1078

ABC_RNaseL_inhibitor_domain2; The ATP-binding cassette domain 2 of RNase L inhibitor
NM_002940.2 → NP_002931.2 ATP-binding cassette sub-family E member 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC096757, BC016283, DA218189

Consensus CDS

CCDS34071.1

UniProtKB/Swiss-Prot

P61221

Related

ENSP00000296577, OTTHUMP00000220305, ENST00000296577, OTTHUMT00000365104

Conserved Domains (4) summary

COG1245
Location:4 – 597
Blast Score: 2116

COG1245; Predicted ATPase, RNase L inhibitor (RLI) homolog [General function prediction only]

pfam04068
Location:6 – 37
Blast Score: 128

RLI; Possible Fer4-like domain in RNase L inhibitor, RLI

cd03236
Location:78 – 332
Blast Score: 1214

ABC_RNaseL_inhibitor_domain1; The ATP-binding cassette domain 1 of RNase L inhibitor.

cd03237
Location:359 – 592
Blast Score: 1078

ABC_RNaseL_inhibitor_domain2; The ATP-binding cassette domain 2 of RNase L inhibitor

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000004.11 Reference GRCh37.p10 Primary Assembly

Range

146019156..146050676

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000136.1 Alternate HuRef

Range

141750477..141781999

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018915.1 Alternate CHM1_1.0

Range

145804790..145836309

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01070024.1 (59274..90796)	None
genomic	AC096757.3 (65304..96500)	None
genomic	AMYH01014592.1 (70817..102336)	None
genomic	CH471056.2	EAX05049.1
genomic	DQ148409.1	AAZ38723.1
genomic	L24115.1	None
mRNA	AB062293.1	BAB93476.1
mRNA	AK315198.1	None
mRNA	BC016283.1	AAH16283.1
mRNA	BC016988.1	AAH16988.1
mRNA	BT009779.1	AAP88781.1
mRNA	DA218189.1	None
mRNA	X74987.1	CAA52920.1
mRNA	X76388.1	CAA53972.1