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    PYGM phosphorylase, glycogen, muscle [ Homo sapiens ]

    Gene ID: 5837, updated on 11-May-2012

    Summary

    Official Symbol
    PYGMprovided by HGNC
    Official Full Name
    phosphorylase, glycogen, muscleprovided by HGNC
    Primary source
    HGNC:9726
    See related
    Ensembl:ENSG00000068976; HPRD:01986; MIM:608455; Vega:OTTHUMG00000066835
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

    Genomic context

    Location :
    11q12-q13.2
    Sequence :
    Chromosome: 11; NC_000011.9 (64513861..64528187, complement)
    See PYGM in Epigenomics, MapViewer

    Chromosome 11 - NC_000011.9Genomic Context describing neighboring genes Neighboring gene neurexin 2 Neighboring gene RAS guanyl releasing protein 2 (calcium and DAG-regulated) Neighboring gene splicing factor 1 Neighboring gene mitogen-activated protein kinase kinase kinase kinase 2

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Phenotypes

    Glycogen storage disease type V

    Summary from GeneReviews: Go to GeneReviews

    Disease Characteristics
    Glycogen storage disease type V (GSDV, McArdle disease) is a metabolic myopathy characterized by exercise intolerance manifested by rapid fatigue, myalgia, and cramps in exercising muscles. Symptoms usually are precipitated by isometric exercise and sustained aerobic exercise. Most individuals improve their exercise tolerance by exploiting the "second wind" phenomenon with relief of myalgia and fatigue after a few minutes of rest. Onset of GSDV typically occurs in the second to third decade of life; however, a severe, rapidly progressive form manifests shortly after birth. Non-progressive weakness occurs in approximately one third of affected individuals, is more likely to involve proximal muscles, and is more common in individuals of advanced age. In some individuals, progressive weakness manifests in the sixth or seventh decade of life. Approximately 50% of individuals have episodes of myoglobinuria that can result in acute renal failure.
    Diagnosis Testing
    GSDV is diagnosed by clinical findings, supportive laboratory findings (i.e., increased resting serum creatine kinase [CK] concentration and no change in plasma lactate concentration on the forearm non-ischemic or ischemic test), and the cycle test (a specific, sensitive, and simple test that is based on the pathognomonic heart rate response observed in the second wind phenomenon). The diagnosis is confirmed either by assay of myophosphorylase enzyme activity or by molecular genetic testing of PYGM (encoding glycogen phosphorylase, muscle form), the only gene associated with GSDV. Targeted mutation analysis of the most common mutations, p.Arg50X and p.Gly205Ser, and sequence analysis of the entire coding region are available on a clinical basis.
    Genetic Counseling
    GSDV is inherited in an autosomal recessive manner. At conception, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being a carrier, and a 25% chance of being unaffected and not a carrier. Once an at-risk sib is known to be unaffected, the risk of his/her being a carrier is 2/3. Heterozygotes are generally asymptomatic. Carrier testing for at-risk family members and prenatal diagnosis for pregnancies at increased risk are possible if the mutations have been identified in the family.
    References

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P11217 P11217 PYGM    HPRD  PubMed  
    P11217 P23297 S100A1    HPRD  PubMed  
    BioGRID:111795 BioGRID:232850 Ccdc15    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111795 BioGRID:114130 DEGS1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111795 BioGRID:118896 PACSIN3    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111795 BioGRID:112179 S100A1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:111795 BioGRID:124195 TRIM63    BioGRID  PubMed Two-hybrid 
    BioGRID:111795 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 

    General gene information

    Markers

    Homology

    • Homologs of the PYGM gene: The PYGM gene is conserved in chimpanzee, , dog, cow, mouse, rat, zebrafish, fruit fly, mosquito, C.elegans, A.thaliana, and rice.
    • Map Viewer (Mouse, Rat)

    Pathways from BioSystems

    • Glucose metabolism, organism-specific biosystem (from REACTOME)
      Glucose metabolism, organism-specific biosystemGlucose is the major form in which dietary sugars are made available to cells of the human body. Its breakdown is a major source of energy for all cells, and is essential for the brain and red blood ...
    • Glycogen Metabolism, organism-specific biosystem (from WikiPathways)
      Glycogen Metabolism, organism-specific biosystemGlycogen is a very large, branched polymer of glucose residues. Within skeletal musle and liver glucose is stored as glycogen. In the liver, glycogen synthesis and degradation are regulated to mainta...
    • Glycogen breakdown (glycogenolysis), organism-specific biosystem (from REACTOME)
      Glycogen breakdown (glycogenolysis), organism-specific biosystemGlycogen breakdown occurs via the same chemical steps in all tissues but is separately regulated via tissue specific isozymes and signaling pathways that enable distinct physiological fates for liver...
    • Insulin signaling pathway, organism-specific biosystem (from KEGG)
      Insulin signaling pathway, organism-specific biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Insulin signaling pathway, conserved biosystem (from KEGG)
      Insulin signaling pathway, conserved biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Metabolism of carbohydrates, organism-specific biosystem (from REACTOME)
      Metabolism of carbohydrates, organism-specific biosystemThese pathways together are responsible for: 1) the extraction of energy and carbon skeletons for biosyntheses from dietary sugars and related molecules; 2) the short-term storage of glucose in the b...
    • Starch and sucrose metabolism, organism-specific biosystem (from KEGG)
      Starch and sucrose metabolism, organism-specific biosystem
      Starch and sucrose metabolism
    • Starch and sucrose metabolism, conserved biosystem (from KEGG)
      Starch and sucrose metabolism, conserved biosystem
      Starch and sucrose metabolism

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    AMP binding IEA
    Inferred from Electronic Annotation
    more info
     
    drug binding IEA
    Inferred from Electronic Annotation
    more info
     
    glycogen phosphorylase activity IEA
    Inferred from Electronic Annotation
    more info
     
    nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
     
    pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
     
    sugar binding IEA
    Inferred from Electronic Annotation
    more info
     
    transferase activity, transferring glycosyl groups IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    carbohydrate metabolic process TAS
    Traceable Author Statement
    more info
     
    cellular calcium ion homeostasis IEA
    Inferred from Electronic Annotation
    more info
     
    glucose metabolic process TAS
    Traceable Author Statement
    more info
     
    glycogen catabolic process TAS
    Traceable Author Statement
    more info
     
    glycogen metabolic process TAS
    Traceable Author Statement
    more info
    PubMed 
    response to cAMP IEA
    Inferred from Electronic Annotation
    more info
     
    response to hypoxia IEA
    Inferred from Electronic Annotation
    more info
     
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    cytosol TAS
    Traceable Author Statement
    more info
     
    sarcoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
     
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    glycogen phosphorylase, muscle form
    Names
    glycogen phosphorylase, muscle form
    myophosphorylase
    NP_001158188.1
    NP_005600.1

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_013018.1 RefSeqGene

      Range
      5001..19327
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001164716.1NP_001158188.1  glycogen phosphorylase, muscle form isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks two exons uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1.
      Source sequence(s)
      AK056607, AP001462, BM987822
      Consensus CDS
      CCDS53659.1
      UniProtKB/Swiss-Prot
      P11217
      Related
      ENSP00000366650, OTTHUMP00000069756, ENST00000377432, OTTHUMT00000143255
      Conserved Domains (2) summary
      cd04300
      Location:82740
      Blast Score: 3301
      GT1_Glycogen_Phosphorylase; This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of ...
      cl10013
      Location:2983
      Blast Score: 232
      Glycosyltransferase_GTB_type; Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate ...
    2. NM_005609.2NP_005600.1  glycogen phosphorylase, muscle form isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
      Source sequence(s)
      AH006714, AJ572752, BC126392
      Consensus CDS
      CCDS8079.1
      UniProtKB/Swiss-Prot
      P11217
      Related
      ENSP00000164139, OTTHUMP00000069755, ENST00000164139, OTTHUMT00000143254
      Conserved Domains (2) summary
      cd04300
      Location:29828
      Blast Score: 3856
      GT1_Glycogen_Phosphorylase; This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of ...
      TIGR02093
      Location:32828
      Blast Score: 3423
      P_ylase; glycogen/starch/alpha-glucan phosphorylases

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000011.9 Reference GRCh37.p5 Primary Assembly

      Range
      64513861..64528187, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000143.1 Alternate HuRef

      Range
      60840630..60855032, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AP001462.3 (30831..44989) None
    genomic CH471076.1 EAW74284.1
      EAW74285.1
    genomic M32598.1 AAA60231.1
    genomic U94774.1 AAC52081.1
    genomic U94777.1 AAC52081.1
    mRNA AF066859.1 AAC17451.1
    mRNA AJ572752.1 None
    mRNA AK056607.1 BAG51762.1
    mRNA AK057547.1 None
    mRNA AK299376.1 BAG61366.1
    mRNA AK299461.1 BAG61428.1
    mRNA AK314474.1 BAG37079.1
    mRNA BC029305.1 None
    mRNA BC126392.1 AAI26393.1
    mRNA BC130514.1 AAI30515.1
    mRNA BM987822.1 None
    mRNA M16013.1 AAA36216.1
    mRNA X03031.1 CAA26834.1
    other-genetic HQ258678.1 ADR83429.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P11217.6 GenPept UniProtKB/Swiss-Prot:P11217

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