PURB purine-rich element binding protein B [Homo sapiens (human)] - Gene

Summary

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Official Symbol: PURBprovided by HGNC
Official Full Name: purine-rich element binding protein Bprovided by HGNC
Primary source: HGNC:9702
See related: Ensembl:ENSG00000146676; HPRD:12325; MIM:608887; Vega:OTTHUMG00000023578
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PURBETA
Summary: This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Genomic context

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Location :: 7p13

Sequence :: Chromosome: 7; NC_000007.13 (44915892..44924960, complement)

See PURB in Epigenomics, MapViewer

Chromosome 7 - NC_000007.13 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Human variation in alcohol response is influenced by variation in neuronal signaling genes.
findings offer insights into mechanism, energetics, and sequence of Purbeta repressor binding to a biologically relevant, contractile phenotype-regulating cis-element while revealing the thermodynamic confines of Purbeta-mediated effects on DNA structure
Title: Mechanism of strand-specific smooth muscle alpha-actin enhancer interaction by purine-rich element binding protein B (Purbeta).
results show that PUR proteins are capable of binding to alpha-MHC mRNA and attenuate its translational efficiency; also show robust expression of PUR proteins in failing hearts where alpha-MHC mRNA levels are suppressed
Title: Single-stranded DNA-binding proteins PURalpha and PURbeta bind to a purine-rich negative regulatory element of the alpha-myosin heavy chain gene and control transcriptional and translational regulation of the gene expression. Implications in the repression of alpha-myosin heavy chain during heart failure.

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Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Rev, p19	rev	HIV-1 Rev interacting protein, purine-rich element binding protein B (PURB), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with PURB is increased by RRE	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
Q96QR8	Q00577	PURA	HPRD	PubMed
BioGRID:111773	BioGRID:125959	ALDH16A1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111773	BioGRID:107841	CSNK2A1	BioGRID	PubMed	Biochemical Activity
BioGRID:111773	BioGRID:114030	CUL3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111773	BioGRID:108309	ELAVL1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:111773	BioGRID:109789	IL7R	BioGRID	PubMed	Protein-RNA
BioGRID:111773	BioGRID:111420	POLD1	BioGRID	PubMed	Co-fractionation
BioGRID:111773	BioGRID:111456	POU5F1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:111773	BioGRID:111772	PURA	BioGRID	PubMed	Co-fractionation
BioGRID:111773	BioGRID:111915	RFC3	BioGRID	PubMed	Co-fractionation
BioGRID:111773	BioGRID:112071	RPL24	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111773	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111773	BioGRID:1205542	rev	BioGRID	PubMed	Affinity Capture-MS

General gene information

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Markers

D10S275 (e-PCR)
- UniSTS:147992

NoName (e-PCR)
- UniSTS:483353

D8S2279 (e-PCR)
- UniSTS:473907

L18426 (e-PCR)
- UniSTS:34648

NoName (e-PCR)
- UniSTS:487017

SHGC-31275 (e-PCR)
- UniSTS:29808

NoName (e-PCR)
- UniSTS:480909

D16S325 (e-PCR)
- UniSTS:147089

A006U23 (e-PCR)
- UniSTS:14755

D7S2274 (e-PCR)
- UniSTS:78761

SGC32761 (e-PCR)
- UniSTS:1239

AA859841 (e-PCR)
- UniSTS:243947

Homology

Homologs of the PURB gene: The PURB gene is conserved in chimpanzee, cow, mouse, rat, zebrafish, fruit fly, mosquito, C.elegans, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
SMAD binding	IEA Inferred from Electronic Annotation more info
double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
mRNA binding	ISS Inferred from Sequence or Structural Similarity more info
sequence-specific DNA binding transcription factor activity	IEA Inferred from Electronic Annotation more info
single-stranded DNA binding	ISS Inferred from Sequence or Structural Similarity more info
transcription factor binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
negative regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
regulation of myeloid cell differentiation	NAS Non-traceable Author Statement more info	PubMed
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
DNA replication factor A complex	ISS Inferred from Sequence or Structural Similarity more info
nucleus	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: transcriptional activator protein Pur-beta

Names: transcriptional activator protein Pur-beta; purine-rich element-binding protein B

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_033224.3 → NP_150093.1 transcriptional activator protein Pur-beta

Status: REVIEWED

Source sequence(s)

AA016115, AI050971, AI209043, AI912557, AK054977, AK056651, AK057669, AL119133, AL705373, AY039216, BF964815, BG776957, BM015566, BQ890213, BU154650, BU177276, CB046374, R89560

Consensus CDS

CCDS5499.1

UniProtKB/Swiss-Prot

Q96QR8

Related

ENSP00000379051, OTTHUMP00000159538, ENST00000395699, OTTHUMT00000251332

Conserved Domains (2) summary

pfam04845
Location:31 – 279
Blast Score: 748

PurA; PurA ssDNA and RNA-binding protein

smart00712
Location:33 – 95
Blast Score: 231

PUR; DNA/RNA-binding repeats in PUR-alpha/beta/gamma and in hypothetical proteins from spirochetes and the Bacteroides-Cytophaga-Flexibacter bacteria.

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000007.13 Reference GRCh37.p10 Primary Assembly

Range

44915892..44924960, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000139.1 Alternate HuRef

Range

44801230..44810302, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CRA_TCAGchr7v2

Genomic

AC_000068.1 Alternate CRA_TCAGchr7v2

Range

44955325..44964397, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018918.1 Alternate CHM1_1.0

Range

44891786..44900858, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AACC02000110.1 (750407..759479)	None
genomic	ABBA01058239.1 (7053..16125)	None
genomic	AC004854.3	AAS00366.1
genomic	AMYH01017219.1 (4459..13531)	None
genomic	CH236960.1	EAL23749.1
genomic	CH471128.2	EAW61073.1
mRNA	AA016115.1	None
mRNA	AI050971.1	None
mRNA	AI209043.1	None
mRNA	AI912557.1	None
mRNA	AK054977.1	None
mRNA	AK056651.1	None
mRNA	AK057669.1	None
mRNA	AL119133.1	None
mRNA	AL705373.1	None
mRNA	AY039216.1	AAK72462.1
mRNA	BC101735.1	AAI01736.1
mRNA	BC101737.1	AAI01738.1
mRNA	BF964815.1	None
mRNA	BG776957.1	None
mRNA	BM015566.1	None
mRNA	BQ890213.1	None
mRNA	BU154650.1	None
mRNA	BU177276.1	None
mRNA	CB046374.1	None
mRNA	R89560.1	None