PURA purine-rich element binding protein A [Homo sapiens] - Gene

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Summary

Official Symbol: PURAprovided by HGNC
Official Full Name: purine-rich element binding protein Aprovided by HGNC
Primary source: HGNC:9701
Locus tag: hCG_45299
See related: Ensembl:ENSG00000185129; HPRD:02721; MIM:600473; Vega:OTTHUMG00000129242
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PUR1; PURALPHA; PUR-ALPHA
Summary: This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 5q31

Sequence :: Chromosome: 5; NC_000005.9 (139493708..139499001)

See PURA in Epigenomics, MapViewer

Chromosome 5 - NC_000005.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study identifies three functionally distinct PURA promoters and show that these are utilized differentially in human cell types and that they respond differently to cytomegalovirus infection.
Title: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals.
Effects of the DNA damage-inducing cancer chemotherapeutic agent cisplatin on mouse embryo fibroblasts (MEFs) from PURA negative knockout mice.
Title: Protective role of Puralpha to cisplatin.
Regulation of PURalpha expression in prostate cancer cells may serve as a therapeutic target for hormone refractory prostate cancer.
Title: Purine-rich element binding protein (PUR) alpha induces endoplasmic reticulum stress response, and cell differentiation pathways in prostate cancer cells.
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
Title: Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
Pur-alpha protein is associated with Alzheimer's disease.
Title: Negative regulation of AbetaPP gene expression by pur-alpha.
Stably overexpressing PURalpha in PC3 and DU145 cells negatively regulates cell proliferation, resulting in decreases in PCNA expression
Title: The potential role of purine-rich element binding protein (PUR) alpha as a novel treatment target for hormone-refractory prostate cancer.
the partnership of Puralpha with Rev is beneficial for Rev-mediated expression of the HIV-1 genome.
Title: Puralpha as a cellular co-factor of Rev/RRE-mediated expression of HIV-1 intron-containing mRNA.
A transcriptional repressor complex that binds a specific sequence (repressor element) in the androgen recptor gene 5'-untranslated region in prostate cancer contains Pur alpha.
Title: Androgen receptor overexpression in prostate cancer linked to Pur alpha loss from a novel repressor complex.
that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription.
Title: Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription.
Hypoxia induces a coordinated up-regulation in beta2 integrin expression that is dependent upon transcriptional mechanisms mediated by HIF-1 and Pur alpha.
Title: Identification of Pur alpha as a new hypoxia response factor responsible for coordinated induction of the beta 2 integrin family.

Submit: New GeneRIF Correction See all (15)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Expression of HIV-1 Tat in human SK-N-MC cells inhibits the NGF-signaling pathway leading to suppression of MAPK and reduction of Puralpha binding to the Egr-1 promoter	PubMed
	tat	Puralpha interacts with HIV-1 Tat to regulate the effect of Tat transactivating the HIV-1 LTR promoter, as well as the JCV promoter and other cellular promoters	PubMed
	tat	Binding of HIV-1 Tat to Puralpha is mediated through amino acids 49-72 of Tat and 100-121 of Puralpha, and is enhanced by RNA	PubMed
	tat	Puralpha self-associates in an RNA dependent manner, an effect that is inhibited by HIV-1 Tat	PubMed
	tat	Puralpha-mediated inhibition of protein translation can be inhibited by HIV-1 Tat	PubMed
	tat	HIV-1 Tat cooperates with Puralpha, cyclin T1, and CDK9 to activate the TNFalpha promoter and upregulate TNFalpha expression	PubMed
	tat	HIV-1 Tat downregulates the expression of p35, a neuron-specific activator of cdk5, and also binds to Puralpha, which associates with cdk5, leading to deregulation of neuronal differentiation and survival	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_005850.1	NP_043512.1	Jvgp5	BIND	PubMed	Pur-alpha interacts with the JCV T-antigen.
NP_005850.1	NP_004550.2	YBX1	BIND	PubMed	YB-1 interacts with Pur-alpha.
Q00577	O75689	ADAP1	HPRD	PubMed
Q00577	O60563	CCNT1	HPRD	PubMed
Q00577	Q00535	CDK5	HPRD	PubMed
Q00577	Q01094	E2F1	HPRD	PubMed
Q00577	Q96QR8	PURB	HPRD	PubMed
Q00577	P06400	RB1	HPRD	PubMed
Q00577	P08047	SP1	HPRD	PubMed
Q00577	P67809	YBX1	HPRD	PubMed
BioGRID:111772	BioGRID:107343	CCNT1	BioGRID	PubMed	Reconstituted Complex
BioGRID:111772	BioGRID:113387	CNBP	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111772	BioGRID:108201	E2F1	BioGRID	PubMed	Reconstituted Complex
BioGRID:111772	BioGRID:111596	EIF2AK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111772	BioGRID:109420	HNRNPA1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111772	BioGRID:121122	MEPCE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111772	BioGRID:111456	POU5F1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:111772	BioGRID:112550	SP1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:111772	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
SMAD binding	IEA Inferred from Electronic Annotation more info
double-stranded telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
purine-rich negative regulatory element binding	IEA Inferred from Electronic Annotation more info
sequence-specific DNA binding transcription factor activity	IEA Inferred from Electronic Annotation more info
sequence-specific distal enhancer binding RNA polymerase II transcription factor activity	TAS Traceable Author Statement more info	PubMed
single-stranded DNA binding	ISS Inferred from Sequence or Structural Similarity more info
transcription factor binding	ISS Inferred from Sequence or Structural Similarity more info
translation repressor activity, nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
DNA unwinding involved in replication	IDA Inferred from Direct Assay more info	PubMed
DNA-dependent DNA replication initiation	TAS Traceable Author Statement more info	PubMed
negative regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
negative regulation of translation	IEA Inferred from Electronic Annotation more info
nervous system development	IEA Inferred from Electronic Annotation more info
positive regulation of cell proliferation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
DNA replication factor A complex	ISS Inferred from Sequence or Structural Similarity more info
cytoplasm	IEA Inferred from Electronic Annotation more info
dendrite	IEA Inferred from Electronic Annotation more info
neuronal cell body	IEA Inferred from Electronic Annotation more info
nuclear chromosome, telomeric region	IC Inferred by Curator more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: transcriptional activator protein Pur-alpha

Names: transcriptional activator protein Pur-alpha; purine-rich single-stranded DNA-binding protein alpha

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_005859.4 → NP_005850.1 transcriptional activator protein Pur-alpha

Status: REVIEWED

Source sequence(s)

AC011379, CK819077, M96684

Consensus CDS

CCDS4220.1

UniProtKB/Swiss-Prot

Q00577

Related

ENSP00000332706, OTTHUMP00000159547, ENST00000331327, OTTHUMT00000251341

Conserved Domains (2) summary

pfam04845
Location:58 – 278
Blast Score: 909

PurA; PurA ssDNA and RNA-binding protein

cl15305
Location:60 – 122
Blast Score: 228

DUF3276; Protein of unknown function (DUF3276)

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000005.9 Reference GRCh37.p5 Primary Assembly

Range

139493708..139499001

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000137.1 Alternate HuRef

Range

134636880..134642173

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC011379.7 (88287..93013)	None
genomic	CH471062.2	EAW62076.1
mRNA	BC036087.1	AAH36087.1
mRNA	BC110574.1	AAI10575.1
mRNA	BC110594.1	AAI10595.1
mRNA	BT019388.1	AAV38195.1
mRNA	CK819077.1	None
mRNA	M96684.1	AAA60229.1
mRNA	X91648.1	None

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
Q00577.2	GenPept	UniProtKB/Swiss-Prot:Q00577
Q2NLC9	GenPept	UniProtKB/TrEMBL:Q2NLC9
Q2NLD4	GenPept	UniProtKB/TrEMBL:Q2NLD4
Q56A79	GenPept	UniProtKB/TrEMBL:Q56A79
Q5U0P9	GenPept	UniProtKB/TrEMBL:Q5U0P9