crb2b crumbs cell polarity complex component 2b [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: crb2bprovided by ZNC
Official Full Name: crumbs cell polarity complex component 2bprovided by ZNC
Primary source: ZFIN:ZDB-GENE-060610-1
See related: AllianceGenome:ZFIN:ZDB-GENE-060610-1
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: crb2l
Summary: Predicted to enable calcium ion binding activity. Acts upstream of or within several processes, including animal organ development; glomerular filtration; and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Located in plasma membrane. Part of protein-containing complex. Is expressed in nervous system and pronephros. Human ortholog(s) of this gene implicated in focal segmental glomerulosclerosis 9 and ventriculomegaly - cystic kidney disease. Orthologous to human CRB2 (crumbs cell polarity complex component 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: chromosome: 8

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	8	NC_007119.7 (2826599..2910247)
105	previous assembly	GRCz10 (GCF_000002035.5)	8	NC_007119.6 (2767880..2849792)

Chromosome 8 - NC_007119.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish.
Title: Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish.
Mutations in crb2b affect cilia length in a subset of tissues.
Title: Apico-basal Polarity Determinants Encoded by crumbs Genes Affect Ciliary Shaft Protein Composition, IFT Movement Dynamics, and Cilia Length.
Here, we report that the rainbow enhancers activate RGB cone-specific transcription of the ponli and crb2b genes. This study provides a starting point to study how RGB cone-specific transcription defines RGB cones' distinct functions for color vision.
Title: Rainbow Enhancers Regulate Restrictive Transcription in Teleost Green, Red, and Blue Cones.
This study show that the undifferentiated CNS expresses Crb1, Crb2a, and Crb2b in distinct spatial and temporal patterns.
Title: Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.
It was demonstrated that the extracellular domains of Crb2a and Crb2b mediate a cell-cell adhesion function, which plays an essential role in maintaining the integrity of the photoreceptor layer.
Title: Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular domains.
This report is the first to show a role for crb function in podocyte differentiation
Title: A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier.
promotes the formation of apical cell features: photoreceptor inner segments and cilia in renal and auditory systems
Title: oko meduzy and related crumbs genes are determinants of apical cell features in the vertebrate embryo.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within establishment or maintenance of epithelial cell apical/basal polarity	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within eye photoreceptor cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glomerular capillary formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glomerular filtration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glomerulus development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within homophilic cell adhesion via plasma membrane adhesion molecules	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within pancreas morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within photoreceptor cell maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within podocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pronephros development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of non-motile cilium assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of non-motile cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of type B pancreatic cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within retina morphogenesis in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retina morphogenesis in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within slit diaphragm assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: crumbs family member 2b; protein crumbs homolog 2b

Names: crumbs homolog 2, like; crumbs homolog 2b

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001045162.1 → NP_001038627.1 protein crumbs homolog 2b precursor

See identical proteins and their annotated locations for NP_001038627.1

Status: PROVISIONAL

Source sequence(s)

DQ314737

UniProtKB/TrEMBL

Q1A5L1

Conserved Domains (5) summary

smart00179
Location:474 → 500

EGF_CA; Calcium-binding EGF-like domain

cd00054
Location:261 → 297

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:590 → 715

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:1197 → 1227

EGF; EGF-like domain

pfam02210
Location:1044 → 1160

Laminin_G_2; Laminin G domain

RefSeqs of Annotated Genomes: Danio rerio Annotation Release 106 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007119.7 Reference GRCz11 Primary Assembly

Range

2826599..2910247

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017357360.2 → XP_017212849.1 crumbs family member 2b isoform X1

UniProtKB/TrEMBL

A0A8M6Z842

Conserved Domains (5) summary

smart00179
Location:474 → 500

EGF_CA; Calcium-binding EGF-like domain

cd00054
Location:261 → 297

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00110
Location:590 → 715

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...

pfam00008
Location:1197 → 1227

EGF; EGF-like domain

pfam02210
Location:1044 → 1160

Laminin_G_2; Laminin G domain