PBK PDZ binding kinase [Homo sapiens] - Gene

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Summary

Official Symbol: PBKprovided by HGNC
Official Full Name: PDZ binding kinaseprovided by HGNC
Primary source: HGNC:18282
See related: Ensembl:ENSG00000168078; HPRD:17822; MIM:611210; Vega:OTTHUMG00000102113
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: SPK; CT84; TOPK; Nori-3; FLJ14385
Summary: This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 8p21.2

Sequence :: Chromosome: 8; NC_000008.10 (27667138..27695349, complement)

See PBK in Epigenomics, MapViewer

Chromosome 8 - NC_000008.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that overexpression of TOPK could predetermine the metastatic capability of tumors and could serve as a significant prognostic predictor of shortened overall survival and time to recurrence.
Title: Overexpression of T-LAK cell-originated protein kinase predicts poor prognosis in patients with stage I lung adenocarcinoma.
T-LAK cell-originated protein kinase (TOPK) phosphorylation of MKP1 protein prevents solar ultraviolet light-induced inflammation through inhibition of the p38 protein signaling pathway.
Title: T-LAK cell-originated protein kinase (TOPK) phosphorylation of MKP1 protein prevents solar ultraviolet light-induced inflammation through inhibition of the p38 protein signaling pathway.
Upregulation of TOPK is associated with breast cancer.
Title: Critical roles of LGN/GPSM2 phosphorylation by PBK/TOPK in cell division of breast cancer cells.
increased levels of PBK/TOPK may contribute to tumor cell development and progression through suppression of p53 function and consequent reductions in the cell-cycle regulatory proteins such as p21.
Title: PBK/TOPK interacts with the DBD domain of tumor suppressor p53 and modulates expression of transcriptional targets including p21.
Phosphorylation of Prx1 (Ser-32) by TOPK prevents UVB-induced apoptosis in RPMI7951 melanoma cells through regulation of Prx1 peroxidase activity and blockade of intracellular H(2)O(2) accumulation.
Title: T-LAK cell-originated protein kinase (TOPK) phosphorylation of Prx1 at Ser-32 prevents UVB-induced apoptosis in RPMI7951 melanoma cells through the regulation of Prx1 peroxidase activity.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
TOPK is indispensable for cancer cell cytokinesis throughout phosphorylation on p97.
Title: Critical roles of T-LAK cell-originated protein kinase in cytokinesis.
PBK/TOPK protein could serve as a useful indicator for histopathologic differentiation between cholangiocarcinoma and hepatocellular carcinomas and the low expression of PBK/TOPK is predicative of poor survival in cholangiocarcinoma patients.
Title: PBK/TOPK in the differential diagnosis of cholangiocarcinoma from hepatocellular carcinoma and its involvement in prognosis of human cholangiocarcinoma.
Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Molecular genetics of successful smoking cessation: convergent genome-wide association study results.
PBK/TOPK is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells and during normal fetal development.
Title: PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells.

Submit: New GeneRIF Correction See all (15)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
NC_000008.9	NP_001941.2	E2F4	BIND	PubMed	E2F4 interacts with TOPK promoter.
NP_060962.2	NP_001645.1	ARAF	BIND	PubMed	A-RAF interacts with TOPK/PBK.
NP_060962.2	NP_001645.1	ARAF	BIND	PubMed	A-Raf interacts with TOPK.
NP_060962.2	NP_004078.1	DLG1	BIND	PubMed	PBK interacts with DLG1 (SAP97). This interaction was modelled on a demonstrated interaction between PBK from an unspecified species and human DLG1 (SAP97).
NP_060962.2	NP_002871.1	RAF1	BIND	PubMed	RAF1 (C-Raf) interacts with PBK (TOPK).
Q96KB5	P10398	ARAF	HPRD	PubMed
Q96KB5	P14635	CCNB1	HPRD	PubMed
Q96KB5	P06493	CDK1	HPRD	PubMed
Q96KB5	Q12959	DLG1	HPRD	PubMed
Q96KB5	Q5JY77	GPRASP1	HPRD	PubMed
Q96KB5	Q13227	GPS2	HPRD	PubMed
Q96KB5	Q9P2H0	KIAA1377	HPRD	PubMed
Q96KB5	P04049	RAF1	HPRD	PubMed
Q96KB5	Q13485	SMAD4	HPRD	PubMed
Q96KB5	P04637	TP53	HPRD	PubMed
Q96KB5	O94972	TRIM37	HPRD	PubMed
BioGRID:120971	BioGRID:106864	ARAF	BioGRID	PubMed	Two-hybrid
BioGRID:120971	BioGRID:115086	GPRASP1	BioGRID	PubMed	Two-hybrid
BioGRID:120971	BioGRID:109132	GPS2	BioGRID	PubMed	Two-hybrid
BioGRID:120971	BioGRID:109268	H2AFX	BioGRID	PubMed	Biochemical Activity; Reconstituted Complex
BioGRID:120971	BioGRID:121617	KIAA1377	BioGRID	PubMed	Two-hybrid
BioGRID:120971	BioGRID:110678	TRIM37	BioGRID	PubMed	Two-hybrid
BioGRID:120971	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	NAS Non-traceable Author Statement more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
mitosis	NAS Non-traceable Author Statement more info	PubMed
protein phosphorylation	NAS Non-traceable Author Statement more info

Component	Evidence Code	Pubs
cellular_component	ND No biological Data available more info

General protein information

Preferred Names: lymphokine-activated killer T-cell-originated protein kinase

Names: lymphokine-activated killer T-cell-originated protein kinase; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase

NP_060962.2

EC 2.7.12.2

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_018492.2 → NP_060962.2 lymphokine-activated killer T-cell-originated protein kinase

Status: REVIEWED

Source sequence(s)

BC015191, BI461026

Consensus CDS

CCDS6063.1

UniProtKB/Swiss-Prot

Q96KB5

Related

ENSP00000301905, OTTHUMP00000128310, ENST00000301905, OTTHUMT00000219952

Conserved Domains (2) summary

cd00180
Location:39 – 243
Blast Score: 357

PKc; Catalytic domain of Protein Kinases

pfam00069
Location:34 – 316
Blast Score: 351

Pkinase; Protein kinase domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000008.10 Reference GRCh37.p5 Primary Assembly

Range

27667138..27695349, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000140.1 Alternate HuRef

Range

26212013..26240186, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC104997.4 (46491..74702)	None
genomic	CH471080.2	EAW63536.1
		EAW63537.1
mRNA	AB027249.1	BAA99576.1
mRNA	AB027250.1	BAA99577.1
mRNA	AF189722.1	AAF69107.1
mRNA	AF237709.1	AAF71521.1
mRNA	AK027291.1	BAB55019.1
mRNA	AK301836.1	BAG63280.1
mRNA	BC015191.1	AAH15191.1
mRNA	BI461026.1	None
other-genetic	DQ893142.2	ABM84068.1
other-genetic	DQ896427.2	ABM87426.1