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    MCM10 minichromosome maintenance complex component 10 [ Homo sapiens ]

    Gene ID: 55388, updated on 19-May-2012
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    Summary

    Official Symbol
    MCM10provided by HGNC
    Official Full Name
    minichromosome maintenance complex component 10provided by HGNC
    Primary source
    HGNC:18043
    Locus tag
    PRO2249
    See related
    Ensembl:ENSG00000065328; HPRD:11297; MIM:609357; Vega:OTTHUMG00000017694
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CNA43; DNA43; PRO2249; MGC126776
    Summary
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
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    Genomic context

    Location :
    10p13
    Sequence :
    Chromosome: 10; NC_000010.10 (13203554..13253104)
    See MCM10 in Epigenomics, MapViewer

    Chromosome 10 - NC_000010.10Genomic Context describing neighboring genes Neighboring gene BTB (POZ) domain containing 7 pseudogene Neighboring gene ribosomal protein L36a pseudogene 36 Neighboring gene RNA, U6 small nuclear 2 Neighboring gene upper zone of growth plate and cartilage matrix associated

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
    2. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Danielsen JM, et al. Mol Cell Proteomics, 2011 Mar. PMID 21139048.
    3. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
    4. Expanding the substantial interactome of NEMO using protein microarrays. Fenner BJ, et al. PLoS One, 2010 Jan 20. PMID 20098747.
    5. Ultraviolet radiation stress triggers the down-regulation of essential replication factor Mcm10. Sharma A, et al. J Biol Chem, 2010 Mar 12. PMID 20064936.

    See all (41) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
      Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
    2. High doses of ionizing gamma radiation and exposure to a combination of DNA-damaging chemicals do not decrease Mcm10 protein levels, demonstrating that Mcm10 down-regulation is triggered only by UV-specific damage
      Title: Ultraviolet radiation stress triggers the down-regulation of essential replication factor Mcm10.
    3. MCM10 interacts directly with RECQ4 and regulates its DNA unwinding activity.
      Title: MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.
    4. Assembly of the Cdc45-Mcm2-7-GINS complex requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.
      Title: Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.
    5. DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol alpha within the replication fork.
      Title: Physical interactions between Mcm10, DNA, and DNA polymerase alpha.
    6. Observational study of gene-disease association. (HuGE Navigator)
      Title: Genome-wide linkage scans for type 2 diabetes mellitus in four ethnically diverse populations-significant evidence for linkage on chromosome 4q in African Americans: the Family Investigation of Nephropathy and Diabetes Research Group.
    7. Results show that MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers.
      Title: Hexameric ring structure of human MCM10 DNA replication factor.
    8. These results indicate that MCM10 protein is essential for maintaining genome integrity as well as cell cycle progression.
      Title: Knockdown of human MCM10 activates G2 checkpoint pathway.
    9. MCM10 is essential for the efficient elongation step of chromosome replication.
      Title: Knockdown of human MCM10 exhibits delayed and incomplete chromosome replication.
    10. These results argue that cells can tolerate low levels of p180 as long as Mcm10 is present to "recycle" it.
      Title: Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication.
    Submit: New GeneRIF Correction See all (15)
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    Q7L590 NDP52 CALCOCO2    HPRD  PubMed  
    Q7L590 Q15834 CCDC85B    HPRD  PubMed  
    Q7L590 P24385 CCND1    HPRD  PubMed  
    Q7L590 P30281 CCND3    HPRD  PubMed  
    Q7L590 O75419 CDC45    HPRD  PubMed  
    Q7L590 Q99459 CDC5L    HPRD  PubMed  
    Q7L590 Q99741 CDC6    HPRD  PubMed  
    Q7L590 O00311 CDC7    HPRD  PubMed  
    Q7L590 Q00534 CDK6    HPRD  PubMed  
    Q7L590 P38936 CDKN1A    HPRD  PubMed  
    Q7L590 Q8N726 CDKN2A    HPRD  PubMed  
    Q7L590 Q8NHQ1 CEP70    HPRD  PubMed  
    Q7L590 Q9P209 CEP72    HPRD  PubMed  
    Q7L590 Q7Z7J5 DPPA2    HPRD  PubMed  
    Q7L590 Q96CS2 HAUS1    HPRD  PubMed  
    Q7L590 Q96ED9 HOOK2    HPRD  PubMed  
    Q7L590 P19012 KRT15    HPRD  PubMed  
    Q7L590 P49736 MCM2    HPRD  PubMed  
    Q7L590 P25205 MCM3    HPRD  PubMed  
    Q7L590 P33991 MCM4    HPRD  PubMed  
    Q7L590 Q14566 MCM6    HPRD  PubMed  
    Q7L590 P33993 MCM7    HPRD  PubMed  
    Q7L590 KIAA0980 protein NINL    HPRD  PubMed  
    Q7L590 Q13416 ORC2    HPRD  PubMed  
    Q7L590 Q9UBD5 ORC3    HPRD  PubMed  
    Q7L590 O43929 ORC4    HPRD  PubMed  
    Q7L590 O94972 TRIM37    HPRD  PubMed  
    Q7L590 O43298 ZBTB43    HPRD  PubMed  
    Q7L590 Q8NAP8 ZBTB8B    HPRD  PubMed  
    BioGRID:120654 BioGRID:115535 CALCOCO2    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:116198 CCDC85B    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:107336 CCND3    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:120654 BioGRID:123234 CEP70    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:120844 CEP72    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:127407 DPPA2    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:118957 HOOK2    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:114089 IKBKG    BioGRID  PubMed Reconstituted Complex 
    BioGRID:120654 BioGRID:110064 KRT15    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:110187 LMNB1    BioGRID  PubMed Co-fractionation 
    BioGRID:120654 BioGRID:110343 MCM6    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:110344 MCM7    BioGRID  PubMed Co-fractionation 
    BioGRID:120654 BioGRID:201346 Mcm2    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:201349 Mcm6    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:116630 NINL    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:111041 ORC2    BioGRID  PubMed Affinity Capture-Western; Two-hybrid 
    BioGRID:120654 BioGRID:117487 RPAP1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:120654 BioGRID:110678 TRIM37    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:120654 BioGRID:116727 ZBTB43    BioGRID  PubMed Two-hybrid 
    BioGRID:120654 BioGRID:575543 ZBTB8A    BioGRID  PubMed Two-hybrid 
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    General gene information

    Markers

    Genotypes

    Homology

    Pathways from BioSystems

    • Activation of ATR in response to replication stress, organism-specific biosystem (from REACTOME)
      Activation of ATR in response to replication stress, organism-specific biosystemGenotoxic stress caused by DNA damage or stalled replication forks can lead to genomic instability. To guard against such instability, genotoxically-stressed cells activate checkpoint factors that ha...
    • Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
      Activation of the pre-replicative complex, organism-specific biosystemIn S. cerevisiae, two ORC subunits, Orc1 and Orc5, both bind ATP, and Orc1 in addition has ATPase activity. Both ATP binding and ATP hydrolysis appear to be essential functions in vivo. ATP binding b...
    • Cell Cycle, organism-specific biosystem (from REACTOME)
      Cell Cycle, organism-specific biosystem
      Cell Cycle
    • Cell Cycle Checkpoints, organism-specific biosystem (from REACTOME)
      Cell Cycle Checkpoints, organism-specific biosystemA hallmark of the human cell cycle in normal somatic cells is its precision. This remarkable fidelity is achieved by a number of signal transduction pathways, known as checkpoints, which monitor cell...
    • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
      Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
    • DNA Replication, organism-specific biosystem (from WikiPathways)
      DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
    • DNA Replication, organism-specific biosystem (from REACTOME)
      DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
    • DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
      DNA Replication Pre-Initiation, organism-specific biosystemAlthough, DNA replication occurs in the S phase of the cell cycle, the formation of the DNA replication pre-initiation complex begins during G1 phase.
    • G1/S Transition, organism-specific biosystem (from REACTOME)
      G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
    • G2/M Checkpoints, organism-specific biosystem (from REACTOME)
      G2/M Checkpoints, organism-specific biosystemG2/M checkpoints include the checks for damaged DNA, unreplicated DNA, and checks that ensure that the genome is replicated once and only once per cell cycle. If cells pass these checkpoints, they f...
    • M/G1 Transition, organism-specific biosystem (from REACTOME)
      M/G1 Transition, organism-specific biosystemFinally, progression out of mitosis and division of the cell into two daughters (cytokinesis) requires the inactivation of Cyclin B - Cdc2 by ubiquitin-dependent proteolysis of Cyclin A and B, which ...
    • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
      Mitotic G1-G1/S phases, organism-specific biosystem
      Mitotic G1-G1/S phases
    • Mitotic M-M/G1 phases, organism-specific biosystem (from REACTOME)
      Mitotic M-M/G1 phases, organism-specific biosystem
      Mitotic M-M/G1 phases
    • Regulation of DNA replication, organism-specific biosystem (from REACTOME)
      Regulation of DNA replication, organism-specific biosystemDNA replication is regulated at various levels via ORC proteins. This pathway includes annotation of individual events that lead to the regulation of replication.
    • Removal of licensing factors from origins, organism-specific biosystem (from REACTOME)
      Removal of licensing factors from origins, organism-specific biosystemLicensing factors are removed from the origin by various means like biochemical modification (phosphorylation) or by physical association with other proteins. This pathway includes the annotations of...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    DNA replication TAS
    Traceable Author Statement
    more info
    		  
    G1/S transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    M/G1 transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    cell cycle checkpoint TAS
    Traceable Author Statement
    more info
    		  
    mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    Component Evidence Code Pubs
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
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    General protein information

    Preferred Names
    protein MCM10 homolog
    Names
    protein MCM10 homolog
    hsMCM10
    homolog of yeast MCM10
    MCM10 minichromosome maintenance deficient 10
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_018518.4 → NP_060988.3  protein MCM10 homolog isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) encodes the predominant isoform (2).
      Source sequence(s)
      AB042719, AL136840, DA024424
      Consensus CDS
      CCDS7095.1
      UniProtKB/Swiss-Prot
      Q7L590
      Related
      ENSP00000367986, OTTHUMP00000019133, ENST00000378714, OTTHUMT00000046849
      Conserved Domains (2) summary
      pfam09332
      Location:522 – 872
      Blast Score: 1015
      Mcm10; Mcm10 replication factor
      pfam09329
      Location:381 – 426
      Blast Score: 199
      zf-primase; Primase zinc finger

    2. NM_182751.2 → NP_877428.1  protein MCM10 homolog isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) uses a different splice site, compared to variant 2, that results in a longer isoform (1). Though this variant is supported by EST data, the existence of its encoded isoform has not been experimentally verified.
      Source sequence(s)
      AB042719, AK055695, AL136840, DA024424
      Consensus CDS
      CCDS7096.1
      UniProtKB/Swiss-Prot
      Q7L590
      Related
      ENSP00000354945, ENST00000361282
      Conserved Domains (2) summary
      pfam09332
      Location:523 – 873
      Blast Score: 1016
      Mcm10; Mcm10 replication factor
      pfam09329
      Location:382 – 427
      Blast Score: 199
      zf-primase; Primase zinc finger

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000010.10 Reference GRCh37.p5 Primary Assembly

      Range
      13203554..13253104
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000142.1 Alternate HuRef

      Range
      13116201..13165701
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL138764.21 CAI12912.1
      CAI12913.1
    genomic AL355355.25 CAI16557.1
      CAI16558.1
    genomic CH471072.2 EAW86297.1
      EAW86298.1
    mRNA AB042719.1 BAB18723.1
    mRNA AF119869.1 AAF69623.1
    mRNA AK055695.1 BAB70988.1
    mRNA AK292701.1 BAF85390.1
    mRNA AL136840.1 CAB66774.2
    mRNA BC004876.2 AAH04876.2
    mRNA BC009108.1 None
    mRNA BC101727.1 AAI01728.1
    mRNA BC143490.1 AAI43491.1
    mRNA DA024424.1 None
    Protein Accession Links
    GenPept Link UniProtKB Link
    AAB34309.1 GenPept
    AAB34784.1 GenPept
    Q5T670 GenPept UniProtKB/TrEMBL:Q5T670
    Q7L590.2 GenPept UniProtKB/Swiss-Prot:Q7L590
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Medical
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous

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