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POLR2I polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa [ Homo sapiens (human) ]

Gene ID: 5438, updated on 7-Aug-2014
Official Symbol
POLR2Iprovided by HGNC
Official Full Name
polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDaprovided by HGNC
Primary source
HGNC:9196
See related
Ensembl:ENSG00000105258; HPRD:15944; MIM:180662; Vega:OTTHUMG00000181749
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RPB9; hRPB14.5
Summary
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]
See POLR2I in Epigenomics, MapViewer
Location:
19q12
Exon count:
6
Annotation release Status Assembly Chr Location
106 current GRCh38 (GCF_000001405.26) 19 NC_000019.10 (36113709..36115304, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (36604611..36606206, complement)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene THAP domain containing 8 Neighboring gene RNA, Ro-associated Y5 pseudogene 10 Neighboring gene WD repeat domain 62 Neighboring gene ovo-like zinc finger 3 Neighboring gene tubulin folding cofactor B Neighboring gene calpain, small subunit 1 Neighboring gene cytochrome c oxidase subunit VIIa polypeptide 1 (muscle)

Replication interactions

Interaction Pubs
Knockdown of polymerase (RNA) II (DNA directed) polypeptide I (POLR2I, 14.5kDa) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env The transcription factors and kinases c-Jun, JNK, MEK, p38 MAPK, STAT-3, JAK-1, TFII D, TFII F, eIF-4E, and RNA polymerase II are induced by HIV-1 gp120 PubMed
Tat tat A small molecule compound C3 inhibits HIV-1 replication by suppressing HIV-1 Tat-mediated HIV-1 LTR-driven gene expression and phosphorylation of RNAPII through inhibition of Tat binding to CycT1 PubMed
tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed
tat HIV-1 Tat complexes with RNA polymerase II and cellular transcription factors as part of the HIV-1 transcription preinitiation and elongation complexes and stimulates RNA polymerase II processivity by inducing the phosphorylation of its C-terminal Domain PubMed
tat HIV-1 Tat 41/44 peptide TAALS from the core domain of Tat inhibits Tat-mediated HIV-1 gene expression and replication by binding the Cdk2/Cyclin E complex and inhibiting the phosphorylation of serine 5 of RNAPII PubMed
Vpr vpr HIV-1 Vpr interacts with basal transcription complexes such as P-TEFb, TFIIH, TFIID (TBP) and TFIIB which activate RNA polymerase II gene transcription PubMed

Go to the HIV-1, Human Protein Interaction Database

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA binding IEA
Inferred from Electronic Annotation
more info
 
contributes_to DNA-directed RNA polymerase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
zinc ion binding IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
DNA-directed RNA polymerase II, core complex IDA
Inferred from Direct Assay
more info
PubMed 
NOT nucleolus IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
 
Preferred Names
DNA-directed RNA polymerase II subunit RPB9
Names
DNA-directed RNA polymerase II subunit RPB9
RPB14.5
RNA polymerase II subunit B9
RNA polymerase II 14.5 kDa subunit
DNA-directed RNA polymerase II subunit I
DNA-directed RNA polymerase II 14.5 kDa polypeptide
NP_006224.1

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_006233.4NP_006224.1  DNA-directed RNA polymerase II subunit RPB9

    See proteins identical to NP_006224.1

    Status: REVIEWED

    Source sequence(s)
    BC017112, BC067794
    Consensus CDS
    CCDS12487.1
    UniProtKB/Swiss-Prot
    P36954
    Related
    ENSP00000221859, OTTHUMP00000267375, ENST00000221859, OTTHUMT00000457442
    Conserved Domains (3) summary
    smart00661
    Location:1568
    Blast Score: 172
    RPOL9; RNA polymerase subunit 9
    COG1594
    Location:14125
    Blast Score: 227
    RPB9; DNA-directed RNA polymerase, subunit M/Transcription elongation factor TFIIS [Transcription]
    cd10508
    Location:74124
    Blast Score: 239
    Zn-ribbon_RPB9; C-terminal zinc ribbon domain of RPB9 subunit of RNA polymerase II

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38 Primary Assembly

Genomic

  1. NC_000019.10 

    Range
    36113709..36115304
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

  1. AC_000151.1 

    Range
    33110097..33111692
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.1

Genomic

  1. NC_018930.2 

    Range
    36605953..36607548
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)