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POLR2H polymerase (RNA) II subunit H [ Homo sapiens (human) ]

Gene ID: 5437, updated on 12-May-2016
Official Symbol
POLR2Hprovided by HGNC
Official Full Name
polymerase (RNA) II subunit Hprovided by HGNC
Primary source
HGNC:HGNC:9195
See related
Ensembl:ENSG00000163882 HPRD:09082; MIM:606023; Vega:OTTHUMG00000156746
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RPB8; RPB17; RPABC3
Summary
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Orthologs
Location:
3q28
Exon count:
6
Annotation release Status Assembly Chr Location
107 current GRCh38.p2 (GCF_000001405.28) 3 NC_000003.12 (184361714..184368595)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (184079502..184086383)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene eukaryotic translation initiation factor 4 gamma 1 Neighboring gene small nucleolar RNA, C/D box 66 Neighboring gene family with sequence similarity 131 member A Neighboring gene chloride voltage-gated channel 2 Neighboring gene thrombopoietin Neighboring gene chordin

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Replication interactions

Interaction Pubs
Knockdown of polymerase (RNA) II (DNA directed) polypeptide H (POLR2H) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env The transcription factors and kinases c-Jun, JNK, MEK, p38 MAPK, STAT-3, JAK-1, TFII D, TFII F, eIF-4E, and RNA polymerase II are induced by HIV-1 gp120 PubMed
Tat tat The interaction of Tip110 with HIV-1 Tat leads to a higher efficiency of elongation for RNAPII complexes formed on the LTR promoter PubMed
tat Expression of both Tip110 and Tat enhances phosphorylation of RNAPII at both serine 2 and serine 5, which account for Tip110 function in transcription activation PubMed
tat The interaction of Tip110 with HIV-1 Tat and the RNAPII C-terminal domain leads to the recruitment of increased CDK9/CycT1 to the transcription complex PubMed
tat HIV-1 Tat complexes with RNA polymerase II and cellular transcription factors as part of the HIV-1 transcription preinitiation and elongation complexes and stimulates RNA polymerase II processivity by inducing the phosphorylation of its C-terminal Domain PubMed
tat A small molecule compound C3 inhibits HIV-1 replication by suppressing HIV-1 Tat-mediated HIV-1 LTR-driven gene expression and phosphorylation of RNAPII through inhibition of Tat binding to CycT1 PubMed
tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed
tat HIV-1 Tat upregulates the transcription by RNA polymerase III of co-transfected or endogenous cellular Alu-repeated sequences by activating transcription factor TFIIIC PubMed
Vpr vpr HIV-1 Vpr interacts with basal transcription complexes such as P-TEFb, TFIIH, TFIID (TBP) and TFIIB which activate RNA polymerase II gene transcription PubMed

Go to the HIV-1, Human Interaction Database

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA binding IEA
Inferred from Electronic Annotation
more info
 
contributes_to RNA polymerase I activity IBA
Inferred from Biological aspect of Ancestor
more info
 
contributes_to RNA polymerase II activity IBA
Inferred from Biological aspect of Ancestor
more info
 
contributes_to RNA polymerase III activity IBA
Inferred from Biological aspect of Ancestor
more info
 
Process Evidence Code Pubs
7-methylguanosine mRNA capping TAS
Traceable Author Statement
more info
 
fibroblast growth factor receptor signaling pathway TAS
Traceable Author Statement
more info
 
gene expression TAS
Traceable Author Statement
more info
 
gene silencing by RNA TAS
Traceable Author Statement
more info
 
mRNA splicing, via spliceosome TAS
Traceable Author Statement
more info
 
positive regulation of gene expression, epigenetic TAS
Traceable Author Statement
more info
 
positive regulation of type I interferon production TAS
Traceable Author Statement
more info
 
positive regulation of viral transcription TAS
Traceable Author Statement
more info
 
snRNA transcription from RNA polymerase II promoter TAS
Traceable Author Statement
more info
 
somatic stem cell population maintenance TAS
Traceable Author Statement
more info
 
termination of RNA polymerase I transcription TAS
Traceable Author Statement
more info
 
transcription elongation from RNA polymerase I promoter TAS
Traceable Author Statement
more info
 
transcription elongation from RNA polymerase II promoter TAS
Traceable Author Statement
more info
 
transcription from RNA polymerase II promoter IDA
Inferred from Direct Assay
more info
PubMed 
transcription from RNA polymerase II promoter TAS
Traceable Author Statement
more info
 
transcription from RNA polymerase III promoter IEA
Inferred from Electronic Annotation
more info
 
transcription initiation from RNA polymerase I promoter TAS
Traceable Author Statement
more info
 
transcription initiation from RNA polymerase II promoter TAS
Traceable Author Statement
more info
 
transcription-coupled nucleotide-excision repair TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
DNA-directed RNA polymerase I complex IBA
Inferred from Biological aspect of Ancestor
more info
 
DNA-directed RNA polymerase II, core complex IDA
Inferred from Direct Assay
more info
PubMed 
DNA-directed RNA polymerase III complex IBA
Inferred from Biological aspect of Ancestor
more info
 
cytosol TAS
Traceable Author Statement
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
Preferred Names
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Names
DNA-directed RNA polymerase II subunit H
DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
RPB8 homolog
polymerase (RNA) II (DNA directed) polypeptide H
NP_001265627.1
NP_001265628.1
NP_001265629.1
NP_001265643.1
NP_001265644.1
NP_006223.2

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001278698.1NP_001265627.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 1

    See identical proteins and their annotated locations for NP_001265627.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AA431357, BG529732, BX283032, CN368789, Z49199
    Consensus CDS
    CCDS63859.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000415536, OTTHUMP00000210512, ENST00000429568, OTTHUMT00000345566
    Conserved Domains (1) summary
    pfam03870
    Location:7112
    RNA_pol_Rpb8; RNA polymerase Rpb8
  2. NM_001278699.1NP_001265628.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 3

    See identical proteins and their annotated locations for NP_001265628.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. Variants 3 and 4 encode the same isoform (3), which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AA431357, BX283032, CN368789, HY164762, U37689, Z49199
    Consensus CDS
    CCDS63861.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000399882, OTTHUMP00000210511, ENST00000452961, OTTHUMT00000345565
    Conserved Domains (1) summary
    pfam03870
    Location:1111
    RNA_pol_Rpb8; RNA polymerase Rpb8
  3. NM_001278700.1NP_001265629.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 3

    See identical proteins and their annotated locations for NP_001265629.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) contains an alternate 5' terminal exon, initiates translation at a downstream start codon and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. Variants 3 and 4 encode the same isoform (3), which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AA431357, BM541682, BX283032, HY178178, Z49199
    Consensus CDS
    CCDS63861.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000398622, OTTHUMP00000210507, ENST00000438240, OTTHUMT00000345560
    Conserved Domains (1) summary
    pfam03870
    Location:1111
    RNA_pol_Rpb8; RNA polymerase Rpb8
  4. NM_001278714.1NP_001265643.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 4

    See identical proteins and their annotated locations for NP_001265643.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AI244238, BQ065952, BX283032, CN368789
    Consensus CDS
    CCDS63860.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000411883, OTTHUMP00000210509, ENST00000430783, OTTHUMT00000345563
    Conserved Domains (1) summary
    pfam03870
    Location:7119
    RNA_pol_Rpb8; RNA polymerase Rpb8
  5. NM_001278715.1NP_001265644.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 5

    See identical proteins and their annotated locations for NP_001265644.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR and has multiple coding region differences compared to variant 1, one of which results in a frameshift. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AA431357, BI603667, BQ065952, BX283032, CN368789, Z49199
    Consensus CDS
    CCDS63862.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000393773, OTTHUMP00000210510, ENST00000443489, OTTHUMT00000345564
    Conserved Domains (1) summary
    cl02716
    Location:183
    RNA_pol_Rpb8; RNA polymerase Rpb8
  6. NM_006232.3NP_006223.2  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 2

    See identical proteins and their annotated locations for NP_006223.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AI244238, BC000739, BX283032, CN368789, Z49199
    Consensus CDS
    CCDS3264.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000409065, OTTHUMP00000210508, ENST00000455712, OTTHUMT00000345562
    Conserved Domains (1) summary
    pfam03870
    Location:7147
    RNA_pol_Rpb8; RNA polymerase Rpb8

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 107 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p2 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p2 Primary Assembly

    Range
    184361714..184368595
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006713670.2XP_006713733.1  

    See identical proteins and their annotated locations for XP_006713733.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    pfam03870
    Location:1111
    RNA_pol_Rpb8; RNA polymerase Rpb8
  2. XM_006713666.2XP_006713729.1  

    See identical proteins and their annotated locations for XP_006713729.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    pfam03870
    Location:7112
    RNA_pol_Rpb8; RNA polymerase Rpb8
  3. XM_006713668.2XP_006713731.1  

    See identical proteins and their annotated locations for XP_006713731.1

    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000392913, OTTHUMP00000210505, ENST00000456318, OTTHUMT00000345558
    Conserved Domains (1) summary
    pfam03870
    Location:7147
    RNA_pol_Rpb8; RNA polymerase Rpb8
  4. XM_006713667.2XP_006713730.1  

    See identical proteins and their annotated locations for XP_006713730.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    pfam03870
    Location:7112
    RNA_pol_Rpb8; RNA polymerase Rpb8
  5. XM_005247541.2XP_005247598.1  

    See identical proteins and their annotated locations for XP_005247598.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    pfam03870
    Location:7112
    RNA_pol_Rpb8; RNA polymerase Rpb8

Alternate CHM1_1.1

Genomic

  1. NC_018914.2 Alternate CHM1_1.1

    Range
    184043961..184050843
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)