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    PHKA2 phosphorylase kinase, alpha 2 (liver) [ Homo sapiens (human) ]

    Gene ID: 5256, updated on 10-Jun-2013
    Official Symbol
    PHKA2provided by HGNC
    Official Full Name
    phosphorylase kinase, alpha 2 (liver)provided by HGNC
    Primary source
    HGNC:8926
    Locus tag
    RP3-499B10.2
    See related
    Ensembl:ENSG00000044446; HPRD:02380; MIM:300798; Vega:OTTHUMG00000021222
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PHK; PYK; XLG; PYKL; XLG2; GSD9A
    Summary
    Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9A, also known as X-linked liver glycogenosis. Alternatively spliced transcript variants have been reported, but the full-length nature of these variants has not been determined.[provided by RefSeq, Feb 2010]
    Location :
    Xp22.2-p22.1
    Sequence :
    Chromosome: X; NC_000023.10 (18910416..19002480, complement)
    See PHKA2 in Epigenomics, MapViewer

    Chromosome X - NC_000023.10Genomic Context describing neighboring genes Neighboring gene pallidin-like Neighboring gene HAUS augmin-like complex, subunit 1 pseudogene 2 Neighboring gene PHKA2 antisense RNA 1 Neighboring gene G protein-coupled receptor 64 Neighboring gene mitogen-activated protein kinase kinase kinase 15 Neighboring gene pyruvate dehydrogenase (lipoamide) alpha 1

    Related articles in PubMed

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Glycogen storage disease type IXa1

    Summary from GeneReviews: Phosphorylase Kinase Deficiency Go to GeneReviews

    Disease Characteristics
    Phosphorylase kinase (PhK) deficiency causing glycogen storage disease type IX (GSD IX) results from deficiency of the enzyme phosphorylase b kinase, which has a major regulatory role in the breakdown of glycogen. The two types of PhK deficiency are liver PhK deficiency (characterized by early childhood onset of hepatomegaly and growth retardation, and often, but not always, fasting ketosis and hypoglycemia) and muscle PhK deficiency, which is considerably rarer (characterized by any of the following: exercise intolerance, myalgia, muscle cramps, myoglobinuria, and progressive muscle weakness). Symptoms and biochemical abnormalities of liver PhK deficiency are thought to improve with age.
    Diagnosis Testing
    The enzyme PhK comprises four copies each of four subunits (alpha, beta, gamma, and delta). Mutations in PHKA1, encoding subunit alpha, cause the rare X-linked disorder muscle PhK deficiency; mutations in PHKA2, also encoding subunit alpha, cause the most common form, liver PhK deficiency (X-linked liver glycogenosis); mutations in PHKB, encoding subunit beta, cause autosomal recessive PhK deficiency in both liver and muscle; and mutations in PHKG2, encoding subunit gamma, cause autosomal recessive liver PhK deficiency. Diagnosis is based on clinical findings, assay of PhK activity in erythrocytes, or liver or muscle tissues (depending upon presentation) and confirmatory findings on molecular genetic testing.
    Genetic Counseling
    PHKA2-related liver PhK deficiency and PHKA1-related muscle PhK deficiency are inherited in an X-linked manner. PHKB-related liver and muscle PhK deficiency and PHKG2-related liver PhK deficiency are inherited in an autosomal recessive manner. X-linked inheritance: If the mother is a carrier, the chance of transmitting it in each pregnancy is 50%. Males who inherit the mutation will be affected; females who inherit the mutation will be carriers. Affected males pass the disease-causing mutation to all of their daughters and none of their sons. Carrier testing for at-risk female relatives and prenatal testing for pregnancies at risk are possible if the disease-causing mutation in the family has been identified. Autosomal recessive inheritance: At conception, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being an asymptomatic carrier, and a 25% chance of being unaffected and not a carrier. Carrier testing for at-risk relatives and prenatal testing for pregnancies at risk are possible if the disease-causing mutations in the family have been identified.
    References
    Protein Gene Interaction Pubs
    pol gag-pol The alpha subunit of phosphorylase kinase is preferentially cleaved at arg748-val749 by HIV-1 protease PubMed

    Go to the HIV-1, Human Protein Interaction Database

    Products Interactant Other Gene Complex Source Pubs Description
    P46019 O15198 SMAD9    HPRD  PubMed  
    BioGRID:111274 BioGRID:106902 ARRB2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111274 BioGRID:109891 ITGAV    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:111274 BioGRID:115226 KIAA0196    BioGRID  PubMed Co-fractionation 
    BioGRID:111274 BioGRID:122103 MMS19    BioGRID  PubMed Co-fractionation 
    BioGRID:111274 BioGRID:211904 Ndc80    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111274 BioGRID:111831 RAF1    BioGRID  PubMed Two-hybrid 
    BioGRID:111274 BioGRID:110268 SMAD9    BioGRID  PubMed Two-hybrid 
    BioGRID:111274 BioGRID:113185 UBE3A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111274 BioGRID:115043 UBE3C    BioGRID  PubMed Co-fractionation 
    • Calcium signaling pathway, organism-specific biosystem (from KEGG)
      Calcium signaling pathway, organism-specific biosystemCa2+ that enters the cell from the outside is a principal source of signal Ca2+. Entry of Ca2+ is driven by the presence of a large electrochemical gradient across the plasma membrane. Cells use this...
    • Calcium signaling pathway, conserved biosystem (from KEGG)
      Calcium signaling pathway, conserved biosystemCa2+ that enters the cell from the outside is a principal source of signal Ca2+. Entry of Ca2+ is driven by the presence of a large electrochemical gradient across the plasma membrane. Cells use this...
    • Glucose metabolism, organism-specific biosystem (from REACTOME)
      Glucose metabolism, organism-specific biosystemGlucose is the major form in which dietary sugars are made available to cells of the human body. Its breakdown is a major source of energy for all cells, and is essential for the brain and red blood ...
    • Glycogen Metabolism, organism-specific biosystem (from WikiPathways)
      Glycogen Metabolism, organism-specific biosystemGlycogen is a very large, branched polymer of glucose residues. Within skeletal musle and liver glucose is stored as glycogen. In the liver, glycogen synthesis and degradation are regulated to mainta...
    • Glycogen breakdown (glycogenolysis), organism-specific biosystem (from REACTOME)
      Glycogen breakdown (glycogenolysis), organism-specific biosystemGlycogen breakdown occurs via the same chemical steps in all tissues but is separately regulated via tissue specific isozymes and signaling pathways that enable distinct physiological fates for liver...
    • Insulin signaling pathway, organism-specific biosystem (from KEGG)
      Insulin signaling pathway, organism-specific biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Insulin signaling pathway, conserved biosystem (from KEGG)
      Insulin signaling pathway, conserved biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Metabolism of carbohydrates, organism-specific biosystem (from REACTOME)
      Metabolism of carbohydrates, organism-specific biosystemThese pathways together are responsible for: 1) the extraction of energy and carbon skeletons for biosyntheses from dietary sugars and related molecules; 2) the short-term storage of glucose in the b...

    Markers

    Homology

    Clone Names

    • MGC133071

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    calmodulin binding IEA
    Inferred from Electronic Annotation
    more info
     
    hydrolase activity, hydrolyzing O-glycosyl compounds IEA
    Inferred from Electronic Annotation
    more info
     
    phosphorylase kinase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    carbohydrate metabolic process TAS
    Traceable Author Statement
    more info
     
    cellular protein modification process TAS
    Traceable Author Statement
    more info
    PubMed 
    generation of precursor metabolites and energy TAS
    Traceable Author Statement
    more info
    PubMed 
    glucose metabolic process TAS
    Traceable Author Statement
    more info
     
    glycogen catabolic process TAS
    Traceable Author Statement
    more info
     
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytosol TAS
    Traceable Author Statement
    more info
     
    phosphorylase kinase complex TAS
    Traceable Author Statement
    more info
    PubMed 
    plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     
    Preferred Names
    phosphorylase b kinase regulatory subunit alpha, liver isoform
    Names
    phosphorylase b kinase regulatory subunit alpha, liver isoform
    phosphorylase kinase alpha-subunit
    phosphorylase kinase alpha L subunit
    phosphorylase b kinase regulatory subunit alpha liver isoform
    NP_000283.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_016622.1 RefSeqGene

      Range
      5001..97065
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_000292.2NP_000283.1  phosphorylase b kinase regulatory subunit alpha, liver isoform

      Status: REVIEWED

      Source sequence(s)
      BC014036, CD722555, DB458123, W63591, X80497
      Consensus CDS
      CCDS14190.1
      UniProtKB/Swiss-Prot
      P46019
      Related
      ENSP00000369274, OTTHUMP00000023007, ENST00000379942, OTTHUMT00000055960
      Conserved Domains (1) summary
      pfam00723
      Location:9439
      Blast Score: 782
      Glyco_hydro_15; Glycosyl hydrolases family 15

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p10 Primary Assembly

    Genomic

    1. NC_000023.10 Reference GRCh37.p10 Primary Assembly

      Range
      18910416..19002480, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000155.1 Alternate HuRef

      Range
      16663307..16754925, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate CHM1_1.0

    Genomic

    1. NC_018934.1 Alternate CHM1_1.0

      Range
      18824022..18916087, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

      Supplemental Content

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