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    PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ]

    Gene ID: 5105, updated on 20-May-2012

    Summary

    Official Symbol
    PCK1provided by HGNC
    Official Full Name
    phosphoenolpyruvate carboxykinase 1 (soluble)provided by HGNC
    Primary source
    HGNC:8724
    See related
    Ensembl:ENSG00000124253; HPRD:02028; MIM:614168; Vega:OTTHUMG00000032825
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PEPCK1; PEPCKC; PEPCK-C; MGC22652
    Summary
    This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

    Genomic context

    Location :
    20q13.31
    Sequence :
    Chromosome: 20; NC_000020.10 (56136137..56141513)
    See PCK1 in Epigenomics, MapViewer

    Chromosome 20 - NC_000020.10Genomic Context describing neighboring genes Neighboring gene high mobility group box 1 pseudogene 1 Neighboring gene CCCTC-binding factor (zinc finger protein)-like Neighboring gene Z-DNA binding protein 1 Neighboring gene prostate transmembrane protein, androgen induced 1

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    HIV-1 protein interactions

    Protein Gene Interaction Pubs
    integrase gag-pol Interaction of human PCK1 with HIV-1 integrase is presumed based on a reported binding interaction between yeast PCK1 and integrase PubMed

    Go to the HIV-1, Human Protein Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_002582.2 NP_705928.1 gag-pol    BIND  PubMed HIV-1 integrase interacts with PCK1. This interaction was modelled on a demonstrated interaction between HIV-1 integrase and PCK1 from yeast. 
    BioGRID:111136 BioGRID:106540 ABCB7    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:106575 ACTB    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:106977 ATP2A1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:113647 BAG6    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:111136 BioGRID:107147 BSG    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:107169 C1QBP    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:107252 CALM1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:107256 CALM2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:107259 CALM3    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:115827 CCT2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:115826 CCT4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:116603 CCT5    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:107765 CPT1A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114625 DDX21    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:108020 DDX3X    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109534 DNAJA1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:108347 EP300    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:111136 BioGRID:116806 FAF2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:108488 FASN    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:117520 GIGYF2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109030 GNA12    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109032 GNAI1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109040 GNAS    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:115537 GPHN    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109351 HLA-B    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109352 HLA-C    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:133993 HSP90AB2P    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109537 HSPA1L    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:109561 HSPD1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114089 IKBKG    BioGRID  PubMed Reconstituted Complex 
    BioGRID:111136 BioGRID:114048 IRS4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:110131 LDHA    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:122973 LPCAT1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114879 MAGED1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:116121 MAGED2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:124494 MYLK2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:117165 NUP62    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111136 PCK1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111656 PSMA2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111659 PSMA5    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111662 PSMB1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111665 PSMB4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111668 PSMB7    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111673 PSMC1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111674 PSMC2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111677 PSMC4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111678 PSMC5    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111690 PSMD12    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:115508 PSMD14    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:111681 PSMD2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:115195 PSMD6    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:126711 RPL10L    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114507 RPL14    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114752 RPL23    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112044 RPL4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112094 RPLP0    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112124 RPS15A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112102 RPS3    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112103 RPS3A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112108 RPS6    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:116593 SIRT2    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:111136 BioGRID:112391 SKP1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:114397 SQSTM1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:121810 SRPRB    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112783 TCEB1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:112810 TCP1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:108165 TSC22D3    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:111136 BioGRID:119501 UBR5    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:111136 BioGRID:113866 USP11    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:111136 BioGRID:116168 YWHAQ    BioGRID  PubMed Affinity Capture-MS 

    General gene information

    Markers

    Homology

    Pathways from BioSystems

    • Adipocytokine signaling pathway, organism-specific biosystem (from KEGG)
      Adipocytokine signaling pathway, organism-specific biosystemIncreased adipocyte volume and number are positively correlated with leptin production, and negatively correlated with production of adiponectin. Leptin is an important regulator of energy intake and...
    • Adipocytokine signaling pathway, conserved biosystem (from KEGG)
      Adipocytokine signaling pathway, conserved biosystemIncreased adipocyte volume and number are positively correlated with leptin production, and negatively correlated with production of adiponectin. Leptin is an important regulator of energy intake and...
    • Adipogenesis, organism-specific biosystem (from WikiPathways)
      Adipogenesis, organism-specific biosystemThe different classess of factors involved in adipogenesis are shown. Adipogenesis is the process by which fat cells differentiate from predadipocytes to adipocytes (fat cells). Adipose tissue, compo...
    • Citrate cycle (TCA cycle), organism-specific biosystem (from KEGG)
      Citrate cycle (TCA cycle), organism-specific biosystemThe citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form ...
    • Citrate cycle (TCA cycle), conserved biosystem (from KEGG)
      Citrate cycle (TCA cycle), conserved biosystemThe citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form ...
    • Developmental Biology, organism-specific biosystem (from REACTOME)
      Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
    • FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem (from Pathway Interaction Database)
      FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem
      FOXA2 and FOXA3 transcription factor networks
    • Gluconeogenesis, organism-specific biosystem (from REACTOME)
      Gluconeogenesis, organism-specific biosystemThe reactions of gluconeogenesis convert mitochondrial pyruvate to cytosolic glucose 6-phosphate which in turn can be hydrolyzed to glucose and exported from the cell. Gluconeogenesis is confined to ...
    • Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem (from KEGG)
      Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
    • Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem (from KEGG)
      Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
    • Glucose metabolism, organism-specific biosystem (from REACTOME)
      Glucose metabolism, organism-specific biosystemGlucose is the major form in which dietary sugars are made available to cells of the human body. Its breakdown is a major source of energy for all cells, and is essential for the brain and red blood ...
    • Glycolysis / Gluconeogenesis, organism-specific biosystem (from KEGG)
      Glycolysis / Gluconeogenesis, organism-specific biosystemGlycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabol...
    • Glycolysis / Gluconeogenesis, conserved biosystem (from KEGG)
      Glycolysis / Gluconeogenesis, conserved biosystemGlycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabol...
    • Glycolysis and Gluconeogenesis, organism-specific biosystem (from WikiPathways)
      Glycolysis and Gluconeogenesis, organism-specific biosystem
      Glycolysis and Gluconeogenesis
    • Insulin signaling pathway, organism-specific biosystem (from KEGG)
      Insulin signaling pathway, organism-specific biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Insulin signaling pathway, conserved biosystem (from KEGG)
      Insulin signaling pathway, conserved biosystemInsulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the r...
    • Metabolic pathways, organism-specific biosystem (from KEGG)
      Metabolic pathways, organism-specific biosystem
      Metabolic pathways
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Metabolism of carbohydrates, organism-specific biosystem (from REACTOME)
      Metabolism of carbohydrates, organism-specific biosystemThese pathways together are responsible for: 1) the extraction of energy and carbon skeletons for biosyntheses from dietary sugars and related molecules; 2) the short-term storage of glucose in the b...
    • PPAR signaling pathway, organism-specific biosystem (from KEGG)
      PPAR signaling pathway, organism-specific biosystemPeroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that are activated by fatty acids and their derivatives. PPAR has three subtypes (PPARalpha, beta/delta, and gamma) s...
    • PPAR signaling pathway, conserved biosystem (from KEGG)
      PPAR signaling pathway, conserved biosystemPeroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that are activated by fatty acids and their derivatives. PPAR has three subtypes (PPARalpha, beta/delta, and gamma) s...
    • Proximal tubule bicarbonate reclamation, organism-specific biosystem (from KEGG)
      Proximal tubule bicarbonate reclamation, organism-specific biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
    • Proximal tubule bicarbonate reclamation, conserved biosystem (from KEGG)
      Proximal tubule bicarbonate reclamation, conserved biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
    • Pyruvate metabolism, organism-specific biosystem (from KEGG)
      Pyruvate metabolism, organism-specific biosystem
      Pyruvate metabolism
    • Pyruvate metabolism, conserved biosystem (from KEGG)
      Pyruvate metabolism, conserved biosystem
      Pyruvate metabolism
    • Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem (from REACTOME)
      Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystemAdipogenesis is the process of cell differentiation by which preadipocytes become adipocytes. During this process the preadipocytes cease to proliferate, begin to accumulate lipid droplets and develo...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    GDP binding IEA
    Inferred from Electronic Annotation
    more info
     
    GTP binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    carboxylic acid binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    lyase activity IEA
    Inferred from Electronic Annotation
    more info
     
    magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    manganese ion binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    phosphoenolpyruvate carboxykinase (GTP) activity ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    purine nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    carbohydrate metabolic process TAS
    Traceable Author Statement
    more info
     
    gluconeogenesis ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    gluconeogenesis TAS
    Traceable Author Statement
    more info
     
    glucose homeostasis ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    glucose metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    glucose metabolic process TAS
    Traceable Author Statement
    more info
     
    glycerol biosynthetic process from pyruvate ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    internal protein amino acid acetylation IDA
    Inferred from Direct Assay
    more info
     
    lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    oxaloacetate metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    response to activity IEA
    Inferred from Electronic Annotation
    more info
     
    response to insulin stimulus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    cytosol TAS
    Traceable Author Statement
    more info
     
    NOT nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    phosphoenolpyruvate carboxykinase, cytosolic [GTP]
    Names
    phosphoenolpyruvate carboxykinase, cytosolic [GTP]
    PEP carboxykinase
    phosphopyruvate carboxylase
    phosphoenolpyruvate carboxylase
    phosphoenolpyruvate carboxykinase, cytosolic
    NP_002582.3

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_008205.1 RefSeqGene

      Range
      5001..10377
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_002591.3NP_002582.3  phosphoenolpyruvate carboxykinase, cytosolic [GTP]

      Status: REVIEWED

      Source sequence(s)
      AW007566, BC023978, BJ993061, BM758256, DB187814
      Consensus CDS
      CCDS13460.1
      UniProtKB/Swiss-Prot
      P35558
      Related
      ENSP00000319814, OTTHUMP00000031374, ENST00000319441, OTTHUMT00000079851
      Conserved Domains (2) summary
      cd00819
      Location:27615
      Blast Score: 2496
      PEPCK_GTP; Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and ...
      pfam00821
      Location:29622
      Blast Score: 2686
      PEPCK; Phosphoenolpyruvate carboxykinase

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000020.10 Reference GRCh37.p5 Primary Assembly

      Range
      56136137..56141513
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000152.1 Alternate HuRef

      Range
      52917936..52923310
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL035541.15 CAB55863.1
    genomic AY794987.1 AAV50001.1
    genomic CH471077.2 EAW75514.1
      EAW75515.1
    genomic L12760.1 AAA02558.1
    genomic U31519.1 AAA91026.1
    mRNA AI635748.1 None
    mRNA AK290802.1 BAF83491.1
    mRNA AK300072.1 BAG61876.1
    mRNA AK303806.1 BAG64760.1
    mRNA AW007566.1 None
    mRNA BC023978.1 AAH23978.1
    mRNA BJ993061.1 None
    mRNA BM758256.1 None
    mRNA BM768055.1 None
    mRNA BX648510.1 None
    mRNA DB187814.1 None
    mRNA L05144.1 AAA60084.1
    other-genetic DQ893518.2 ABM84444.1
    other-genetic DQ896497.2 ABM87496.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P35558.3 GenPept UniProtKB/Swiss-Prot:P35558

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