VPS36 vacuolar protein sorting 36 homolog (S. cerevisiae) [Homo sapiens (human)] - Gene

Summary

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Official Symbol: VPS36provided by HGNC
Official Full Name: vacuolar protein sorting 36 homolog (S. cerevisiae)provided by HGNC
Primary source: HGNC:20312
Locus tag: CGI-145
See related: Ensembl:ENSG00000136100; HPRD:12620; MIM:610903; Vega:OTTHUMG00000016964
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: EAP45; C13orf9; CGI-145
Summary: VPS36, VPS22 (SNF8; MIM 610904), and VPS25 (MIM 610907) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS36, VPS22, and VPS25 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]

Genomic context

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Location :: 13q14.3

Sequence :: Chromosome: 13; NC_000013.10 (52986737..53024763, complement)

See VPS36 in Epigenomics, MapViewer

Chromosome 13 - NC_000013.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body
Title: Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII.
Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich.
Title: Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
Q86VN1	Q96FZ7	CHMP6	HPRD	PubMed
Q86VN1	Q96H20	SNF8	HPRD	PubMed
Q86VN1	Q99816	TSG101	HPRD	PubMed
Q86VN1	Q9BRG1	VPS25	HPRD	PubMed
BioGRID:119234	BioGRID:122262	ACBD3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:115787	ATG7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:122771	CHMP6	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:113743	CUL5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:108309	ELAVL1	BioGRID	PubMed	Affinity Capture-RNA
BioGRID:119234	BioGRID:109319	HDLBP	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:116966	KIAA0368	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:118875	NXT1	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:111524	PPP3CB	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:123679	RILP	BioGRID	PubMed	Reconstituted Complex
BioGRID:119234	BioGRID:116425	SNF8	BioGRID	PubMed	Affinity Capture-Western; Co-crystal Structure; Two-hybrid
BioGRID:119234	BioGRID:118955	SNX15	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:115547	SPRY2	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:112328	SRSF5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:112982	TNFAIP2	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:125450	TRMT61A	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:113102	TSG101	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:113119	TTC4	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:116472	TWF2	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Co-crystal Structure; Reconstituted Complex
BioGRID:119234	BioGRID:115566	UBE4B	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:113241	USP1	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:35253	VPS20	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:124039	VPS25	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Co-fractionation; Reconstituted Complex; Two-hybrid
BioGRID:119234	BioGRID:33858	VPS25	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:114930	VPS26A	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:119341	VPS28	BioGRID	PubMed	Affinity Capture-Western
BioGRID:119234	BioGRID:119683	VPS29	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:120855	VPS35	BioGRID	PubMed	Co-fractionation
BioGRID:119234	BioGRID:119234	VPS36	BioGRID	PubMed	Two-hybrid
BioGRID:119234	BioGRID:120877	WDR12	BioGRID	PubMed	Affinity Capture-MS
BioGRID:119234	BioGRID:107200	ZNHIT2	BioGRID	PubMed	Co-fractionation

Pathways from BioSystems

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ESCRT-II complex, organism-specific biosystem (from KEGG)
ESCRT-II complex, organism-specific biosystemStructural complex; Genetic information processing; Protein processing
Endocytosis, organism-specific biosystem (from KEGG)
Endocytosis, organism-specific biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
Endocytosis, conserved biosystem (from KEGG)
Endocytosis, conserved biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem (from REACTOME)
Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystemMany plasma membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors are continuously internalized into recycling endosomes and returned to th...
Membrane Trafficking, organism-specific biosystem (from REACTOME)
Membrane Trafficking, organism-specific biosystemThe secretory membrane system allows a cell to regulate delivery of newly synthesized proteins, carbohydrates, and lipids to the cell surface, a necessity for growth and homeostasis. The system is ma...

General gene information

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Markers

A002M11 (e-PCR)
- UniSTS:39710

RH183 (e-PCR)
- UniSTS:79411

D8S2279 (e-PCR)
- UniSTS:473907

SGC32898 (e-PCR)
- UniSTS:19890

Homology

Homologs of the VPS36 gene: The VPS36 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
phosphatidylinositol-3-phosphate binding	IEA Inferred from Electronic Annotation more info
ubiquitin binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cellular membrane organization	TAS Traceable Author Statement more info
endosomal transport	TAS Traceable Author Statement more info
protein transport	IEA Inferred from Electronic Annotation more info
regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cytosol	TAS Traceable Author Statement more info
endosome	IDA Inferred from Direct Assay more info
late endosome	IEA Inferred from Electronic Annotation more info
lysosome	IEA Inferred from Electronic Annotation more info
membrane	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: vacuolar protein-sorting-associated protein 36

Names: vacuolar protein-sorting-associated protein 36; ELL-associated protein, 45 kDa; ESCRT-II complex subunit VPS36; ELL-associated protein of 45 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_016075.2 → NP_057159.2 vacuolar protein-sorting-associated protein 36

Status: VALIDATED

Source sequence(s)

AI352102, AK023182, BC037279, BG400860

Consensus CDS

CCDS9434.1

UniProtKB/Swiss-Prot

Q86VN1

Related

ENSP00000367299, OTTHUMP00000018449, ENST00000378060, OTTHUMT00000045059

Conserved Domains (2) summary

pfam04157
Location:154 – 369
Blast Score: 540

EAP30; EAP30/Vps36 family

pfam11605
Location:3 – 88
Blast Score: 153

Vps36_ESCRT-II; Vacuolar protein sorting protein 36 Vps36

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000013.10 Reference GRCh37.p10 Primary Assembly

Range

52986737..53024763, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000145.1 Alternate HuRef

Range

33725057..33763914, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018924.1 Alternate CHM1_1.0

Range

33924214..33962240, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01006537.1 (49062..53744)	None
genomic	ABBA01006538.1	None
genomic	ABBA01006539.1	None
genomic	AL359513.12 (47804..85830)	None
genomic	AMYH01005122.1 (139736..177762)	None
genomic	BX537689.1	None
genomic	CH471274.1	EAW55895.1
mRNA	AB208956.1	BAD92193.1
mRNA	AF151903.1	AAD34140.1
mRNA	AI352102.1	None
mRNA	AK022911.1	None
mRNA	AK023182.1	BAB14451.1
mRNA	AK289740.1	BAF82429.1
mRNA	BC037279.1	AAH37279.1
mRNA	BC050439.1	AAH50439.1
mRNA	BG400860.1	None
mRNA	BX647847.1	None
mRNA	CR933653.1	CAI45953.1